25 research outputs found
The virobacterial agglutination test as a rapid means of detecting cocoa swollen shoot virus
Immunocapture Polymerase Chain Reaction for the Detection and Characterization of Cacao Swollen Shoot Virus 1 A Isolates
Detection of pineapple bacilliform virus using the polymerase chain reaction
A polymerase chain reaction (PCR) assay has been developed to detect pineapple bacilliform virus (PBV) in extracts from infected plants. Initially, degenerate primers were designed from conserved badnavirus amino acid sequences and used in PCR with partially purified PBV preparations. A 448 bp DNA fragment was amplified from a region in the reverse transcriptase and ribonuclease H genes. The nucleotide sequence of the cloned PCR product indicated that PBV was related to, but distinct from, other badnaviruses. Specific primers designed from the PBV sequence yielded a 403 bp fragment when used in PCR with extracts from infected pineapple plants, but not from plants infected with sugarcane bacilliform or banana streak viruses (genus Badnavirus). The specificity of the PCR product was confirmed by Southern hybridisation using a digoxigenin labelled DNA probe. PBV appears to be present in all pineapple growing areas along the east coast of Australia. PBV was detected in plants grown from seeds, plants propagated through meristem tip culture and in mealybugs which were collected from infected plants. PBV was detected in crown, leaf and root tissue from infected pineapple plants. PCR results of all field-infected samples were confirmed by immunoelectron microscopy
Evidence from the virobacterial agglutination test for the existence of eight serogroups of cocoa swollen shoot virus
Use of gamma rays to induce mutants resistant to cocoa swollen shoot disease in Theobroma cacao L.
Detection and Comparison of some Ghanaian Isolates of Cacao Swollen Shoot Virus (CSSV) by Enzyme-Linked Immunosorbent Assay (ELISA) and Immunoelectron Microscopy (IEM) Using an Antiserum to CSSV Strain 1A
Environmental Controls on the Taphonomy of Phosphatized Animals and Animal Embryos from the Neoproterozoic Doushantuo Formation, Southwest China
Bericht und erste Ergebnisse der Meteor-Fahrt M 16/1 Pointe Noire-Recife, 27.3. - 25.4.1991
With 59 figs., 13 tabs.SIGLEAvailable from TIB Hannover: RO7630(18) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman
Bericht und erste Ergebnisse der Meteor-Fahrt M 16/2 Recife-Belem, 28.4. - 21.5. 1991
With 97 Figs., 11 tabs.SIGLEAvailable from TIB Hannover: RO7630(19) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman