Fall Armyworm (\u3ci\u3eSpodoptera frugiperda\u3c/i\u3e) control by RNAi as a Plausible Component to Integrated Pest Management Practices

Fall armyworm (Spodoptera frugiperda) control through RNAi machinery as a novel mode of action is a weapon sought after by the agricultural community to squelch the growing problem of S. frugiperda resistance to pesticides and transgenic crops. Significant gains in the understanding of RNAi cellular interactions and insect gene silencing are represented to provide opportunities to influence further research of S. frugiperda RNAi. This composition highlights the intracellular action of dsRNA, successful RNAi attempts on insect pests, the biological difficulty of S. frugiperda and how RNAi success against S. frugiperda would be highly advantageous in the quest to mitigate resistance regarding current integrated pest management (IPM) practices to control this polyphagous and genetically plastic agricultural pest

The Variability of Sagittarius A* at Centimeter Wavelengths

We present the results of a 3.3-year project to monitor the flux density of Sagittarius A* at 2.0, 1.3, and 0.7 cm with the VLA. The fully calibrated light curves for Sgr A* at all three wavelengths are presented. Typical errors in the flux density are 6.1%, 6.2%, and 9.2% at 2.0, 1.3, and 0.7 cm, respectively. There is preliminary evidence for a bimodal distribution of flux densities, which may indicate the existence of two distinct states of accretion onto the supermassive black hole. At 1.3 and 0.7 cm, there is a tail in the distribution towards high flux densities. Significant variability is detected at all three wavelengths, with the largest amplitude variations occurring at 0.7 cm. The rms deviation of the flux density of Sgr A* is 0.13, 0.16, and 0.21 Jy at 2.0, 1.3, and 0.7 cm, respectively. During much of this monitoring campaign, Sgr A* appeared to be relatively quiescent compared to results from previous campaigns. At no point during the monitoring campaign did the flux density of Sgr A* more than double its mean value. The mean spectral index of Sgr A* is alpha=0.20+/-0.01, with a standard deviation of 0.14. The spectral index appears to depend linearly on the observed flux density at 0.7 cm with a steeper index observed during outbursts. This correlation is consistent with the expectation for outbursts that are self-absorbed at wavelengths of 0.7 cm or longer and inconsistent with the effects of simple models for interstellar scintillation. Much of the variability of Sgr A*, including possible time lags between flux density changes at the different wavelengths, appears to occur on time scales less than the time resolution of our observations (8 days). Future observations should focus on the evolution of the flux density on these time scales.Comment: 16 pages, 10 figures, accepted for publication in A

Detection of the Intrinsic Size of Sagittarius A* through Closure Amplitude Imaging (includes Online Supporting Material)

We have detected the intrinsic size of Sagittarius A*, the Galactic Center radio source associated with a supermassive black hole, showing that the short-wavelength radio emission arises from very near the event horizon of the black hole. Radio observations with the Very Long Baseline Array show that the source has a size of $24 \pm 2$ Schwarzschild radii at 7 mm wavelength. In one of eight 7-mm epochs we also detect an increase in the intrinsic size of $60^{+25}_{-17}$%. These observations place a lower limit to the mass density of Sgr A* of $1.4\times10^{4}$ solar masses per cubic astronomical unit.Comment: Accepted for publication in Science. Includes the supporting online material that accompanies the main article, which describe further details of the analysis of this experimen

The Nature of the Molecular Environment within 5 pc of the Galactic Center

We present a detailed study of molecular gas in the central 10pc of the Galaxy through spectral line observations of four rotation inversion transitions of NH3 made with the VLA. Updated line widths and NH3(1,1) opacities are presented, and temperatures, column densities, and masses are derived. We examine the impact of Sgr A East on molecular material at the Galactic center and find that there is no evidence that the expansion of this shell has moved a significant amount of the 50 km/s GMC. The western streamer, however, shows strong indications that it is composed of material swept-up by the expansion of Sgr A East. Using the mass and kinematics of the western streamer, we calculate an energy of E=(2-9)x10^{51} ergs for the progenitor explosion and conclude that Sgr A East was most likely produced by a single supernova. The temperature structure of molecular gas in the central ~20pc is also analyzed in detail. We find that molecular gas has a ``two-temperature'' structure similar to that measured by Huttemeister et al. (2003a) on larger scales. The largest observed line ratios, however, cannot be understood in terms of a two-temperature model, and most likely result from absorption of NH3(3,3) emission by cool surface layers of clouds. By comparing the observed NH3 (6,6)-to-(3,3) line ratios, we disentangle three distinct molecular features within a projected distance of 2pc from Sgr A*. Gas associated with the highest line ratios shows kinematic signatures of both rotation and expansion. The southern streamer shows no significant velocity gradients and does not appear to be directly associated with either the circumnuclear disk or the nucleus. The paper concludes with a discussion of the line-of-sight arrangement of the main features in the central 10pc.Comment: 51 pages, 16 figures, accepted for publication in ApJ. Due to size limitations, some of the images have been cut from this version. A complete, color PS or PDF version can be downloaded from http://www.astro.columbia.edu/~herrnstein/NH3/paper

Molecular Tracers of the Central 12 pc of the Galactic Center

We have used the BIMA array to image the Galactic Center with a 19-pointing mosaic in HCN(1-0), HCO+(1-0), and H 42-alpha emission with 5 km/s velocity resolution and 13'' x 4'' angular resolution. The 5' field includes the circumnuclear ring (CND) and parts of the 20 and 50 km/s clouds. HCN(1-0) and HCO+ trace the CND and nearby giant molecular clouds while the H 42-alpha emission traces the ionized gas in Sgr A West. We find that the CND has a definite outer edge in HCN and HCO+ emission at ~45'' radius and appears to be composed of two or three distinct streams of molecular gas rotating around the nucleus. Outside the CND, HCN and HCO+ trace dense clumps of high-velocity gas in addition to optically thick emission from the 20 and 50 km/s clouds. A molecular ridge of compressed gas and dust, traced in NH3 emission and self-absorbed HCN and HCO+, wraps around the eastern edge of Sgr A East. Just inside this ridge are several arcs of gas which have been accelerated by the impact of Sgr A East with the 50 km/s cloud. HCN and HCO+ emission trace the extension of the northern arm of Sgr A West which appears to be an independent stream of neutral and ionized gas and dust originating outside the CND. Broad line widths and OH maser emission mark the intersection of the northern arm and the CND. Comparison to previous NH3 and 1.2mm dust observations shows that HCN and HCO+ preferentially trace the CND and are weaker tracers of the GMCs than NH3 and dust. We discuss possible scenarios for the emission mechanisms and environment at the Galactic center which could explain the differences in these images.Comment: 24 pages, including 17 figures; to appear in The Astrophysical Journa

The Vehicle, Spring 1997

Vol. 38, No. 2 Table of Contents Poetry: Don QuixotePatrick Scanlanpage 1 Last SupperChristine Starrpage 1 Marriage VowsKristopher Clausingpage 2 The LibraryPatrick Lairpage 4 GuruJohn Dylan McNeilpage 5 Tripping in OzKim Evanspage 5 TranceStephanie Kavanaughpage 6 The CleftEmilie Roypage 7 FlannelAmanda Watsonpage 8 Strip PokerEbben Moorepage 8 IceJohn Dylan McNeilpage 9 ChloeMichael Kawapage 11 OrchardCarmella Cosenzapage 12 Jenn & Cookie MonsterJacob Tolbertpage 13 Barry ManilowKatie Wrightpage 14 GoodbyesShannon Goodallpage 15 Prose: Alice (A Short, Short Story)Carmella Cosenzapage 17 UntitledJoe Robesonpage 17 A New World AloneKendall W. Baumanpage 22 Biographiespage 35https://thekeep.eiu.edu/vehicle/1069/thumbnail.jp

Global Distribution of Polaromonas Phylotypes - Evidence for a Highly Successful Dispersal Capacity

Bacteria from the genus Polaromonas are dominant phylotypes in clone libraries and culture collections from polar and high-elevation environments. Although Polaromonas has been found on six continents, we do not know if the same phylotypes exist in all locations or if they exhibit genetic isolation by distance patterns. To examine their biogeographic distribution, we analyzed all available, long-read 16S rRNA gene sequences of Polaromonas phylotypes from glacial and periglacial environments across the globe. Using genetic isolation by geographic distance analyses, including Mantel tests and Mantel correlograms, we found that Polaromonas phylotypes are globally distributed showing weak isolation by distance patterns at global scales. More focused analyses using discrete, equally sampled distances classes, revealed that only two distance classes (out of 12 total) showed significant spatial structuring. Overall, our analyses show that most Polaromonas phylotypes are truly globally distributed, but that some, as yet unknown, environmental variable may be selecting for unique phylotypes at a minority of our global sites. Analyses of aerobiological and genomic data suggest that Polaromonas phylotypes are globally distributed as dormant cells through high-elevation air currents; Polaromonas phylotypes are common in air and snow samples from high altitudes, and a glacial-ice metagenome and the two sequenced Polaromonas genomes contain the gene hipA, suggesting that Polaromonas can form dormant cells

Environmental DNA sequencing primers for eutardigrades and bdelloid rotifers

<p>Abstract</p> <p>Background</p> <p>The time it takes to isolate individuals from environmental samples and then extract DNA from each individual is one of the problems with generating molecular data from meiofauna such as eutardigrades and bdelloid rotifers. The lack of consistent morphological information and the extreme abundance of these classes makes morphological identification of rare, or even common cryptic taxa a large and unwieldy task. This limits the ability to perform large-scale surveys of the diversity of these organisms.</p> <p>Here we demonstrate a culture-independent molecular survey approach that enables the generation of large amounts of eutardigrade and bdelloid rotifer sequence data directly from soil. Our PCR primers, specific to the 18s small-subunit rRNA gene, were developed for both eutardigrades and bdelloid rotifers.</p> <p>Results</p> <p>The developed primers successfully amplified DNA of their target organism from various soil DNA extracts. This was confirmed by both the BLAST similarity searches and phylogenetic analyses. Tardigrades showed much better phylogenetic resolution than bdelloids. Both groups of organisms exhibited varying levels of endemism.</p> <p>Conclusion</p> <p>The development of clade-specific primers for characterizing eutardigrades and bdelloid rotifers from environmental samples should greatly increase our ability to characterize the composition of these taxa in environmental samples. Environmental sequencing as shown here differs from other molecular survey methods in that there is no need to pre-isolate the organisms of interest from soil in order to amplify their DNA. The DNA sequences obtained from methods that do not require culturing can be identified post-hoc and placed phylogenetically as additional closely related sequences are obtained from morphologically identified conspecifics. Our non-cultured environmental sequence based approach will be able to provide a rapid and large-scale screening of the presence, absence and diversity of Bdelloidea and Eutardigrada in a variety of soils.</p