Dietary Canola Oil Suppressed Growth of Implanted MDA-MB 231 Human Breast Tumors in Nude Mice

Long chain omega 3 (n-3) fatty acids, eicosapentaenoic (EPA) and/or docosahexaenoic acid (DHA), have been shown to suppress growth of most cancer cells. In vivo, alpha linolenic acid (ALA, 18:3n-3) can be converted to EPA or DHA. We hypothesized that substituting canola oil (10% ALA) for the corn oil (1% ALA) in the diet of cancer bearing mice would slow tumor growth by increasing n-3 fatty acids in the diet. Sixty nude mice received MDA-MB 231 human breast cancer cells and were fed a diet containing 8% w/w corn oil until the mean tumor volume was 60 mm 3 . The dietary fat of half of the tumor bearing mice was then changed to 8% w/w canola oil. Compared to mice that consumed the corn oil containing diet, the mice that consumed the canola oil containing diet had significantly more EPA and DHA in both tumors and livers, and the mean tumor growth rate and cell proliferation in the tumor were significantly slower (P \u3c 0.05). About 25 days after diet change, mice that consumed the corn oil diet stopped gaining weight, whereas the mice that consumed the canola oil diet continued normal weight gain. Use of canola oil instead of corn oil in the diet may be a reasonable means to increase consumption of n-3 fatty acids with potential significance for slowing growth of residual cancer cells in cancer survivors

Suppression of Implanted MDA-MB 231 Human Breast Cancer Growth in Nude Mice by Dietary Walnut

Walnuts contain components that may slow cancer growth including omega 3 fatty acids, phytosterols, polyphenols, carotenoids, and melatonin. A pilot study was performed to determine whether consumption of walnuts could affect growth of MDA-MB 231 human breast cancers implanted into nude mice. Tumor cells were injected into nude mice that were consuming an AIN-76A diet slightly modified to contain 10% corn oil. After the tumors reached 3 to 5 mm diameter, the diet of one group of mice was changed to include ground walnuts, equivalent to 56 g (2 oz) per day in humans. The tumor growth rate from Day 10, when tumor sizes began to diverge, until the end of the study of the group that consumed walnuts (2.9 ± 1.1 mm3/day; mean ± standard error of the mean) was significantly less (P \u3e 0.05, t-test of the growth rates) than that of the group that did not consume walnuts (14.6 ± 1.3 mm 3 /day). The eicosapentaenoic and docosahexaenoic acid fractions of the livers of the group that consumed walnuts were significantly higher than that of the group that did not consume walnuts. Tumor cell proliferation was decreased, but apoptosis was not altered due to walnut consumption. Further work is merited to investigate applications to cancer in humans

Perancangan Video Iklan Layanan Masyarakat Alat Kontrasepsi Berbasis Online sebagai Sarana Informasi Program Keluarga Berencana

Fungsian failure problems or lack of contraception is still quite common among people who use family planning program. At least the report noted the failure of contraception in the period 2010-2013 amounted to 3846 report (BKKBN). Responding to the issue of the National Family Planning Coordinating Board (BKKBN) gives advice to follow family planning method Long Term (LTM). LTM with contraceptive tool, the contraceptive IUD and implants, it has a higher success rate in controlling the birth rate. In addition to a higher level of security, long-term contraception tool also has a longer lifetime of 5-10 years as user preferences, so that the contraceptive is also considered more efficient in cost pemakiannya. But people still do not know clearly about contraceptives and more use of short-term contraceptives that birth control pills and injectables which also has a lower level of security. Of the existing problems the author aims to design video public service announcements online-based contraceptives as a means of family planning information program

Penciptaan Buku Sejarah Musik Balada dari Masa ke Masa Berbasis Teknik Aquarel sebagai Upaya Pengenalan Kembali pada Masyarakat Surabaya

Seeing the development of music in Surabaya today is very much different from the previous era , there are many musicians from Surabaya are able to vibrate with amazing work . tOne of them is Ballad music genres , there are some big names of Ballads legendary musicians from the city of Surabaya , as Gombloh , Franky Sahilatua , and Leo Kristi . But unfortunately this genre of music began to be abandoned by the people of Surabaya . Especially after Gombloh and Franky died , and Leo Kristi began to move to Bandung . This music is no longer heard in the city of Surabaya . It is also due to the absence participate publish media , in particular through the book . In this study show the history of the development of music Ballad in Surabaya in the 70s until the 2000s by using characters Ballad Surabaya musicians

Dietary Fish Oil Sensitizes A549 Lung Xenografts to Doxorubicin Chemotherapy

A549 xenografts were allowed to grow in nude mice to about 5 mm in diameter, then diets were changed to modified AIN-76 diets containing 19% wt/wt. fish oil (FO) or 20% wt./wt. com oil (CO). Ten days later dietary ferric citrate (0.3% wt./dry wt.) was added and doxoribicin (DOX) treatment (3.6 mg/kg i.v. each of the 5 days for 18 days) commenced. Treatment with DOX halted the growth of tumors in the CO fed mice. However, in those mice, which consumed FO or FO with ferric citrate, treatment with DOX caused significant tumor regression

Omega 3 fatty acids reduce myeloid progenitor cell frequency in the bone marrow of mice and promote progenitor cell differentiation

<p>Abstract</p> <p>Background</p> <p>Omega 3 fatty acids have been found to inhibit proliferation, induce apoptosis, and promote differentiation in various cell types. The processes of cell survival, expansion, and differentiation are of key importance in the regulation of hematopoiesis. We investigated the role of omega 3 fatty acids in controlling the frequency of various myeloid progenitor cells in the bone marrow of mice. Increased progenitor cell frequency and blocked differentiation are characteristics of hematopoietic disorders of the myeloid lineage, such as myeloproliferative diseases and myeloid leukemias.</p> <p>Results</p> <p>We found that increasing the proportion of omega 3 fatty acids relative to the proportion of omega 6 fatty acids in the diet caused increased differentiation and reduced the frequency of myeloid progenitor cells in the bone marrow of mice. Furthermore, this had no adverse effect on peripheral white blood cell counts.</p> <p>Conclusion</p> <p>Our results indicate that omega 3 fatty acids impact hematopoietic differentiation by reducing myeloid progenitor cell frequency in the bone marrow and promoting progenitor cell differentiation. Further exploration of this discovery could lead to the use of omega 3 fatty acids as a therapeutic option for patients that have various disorders of hematopoiesis.</p

Role of Lipid Peroxidation and Antioxidant Enzymes in Omega 3 Fatty Acids Induced Suppression of Breast Cancer Xenograft Growth in Mice

Background: Supplementing mice with high levels of dietary n-3 polyunsaturated fatty acids (PUFAs) increases the n-3 PUFAs in cell membranes, increases the susceptibility of the cells for lipid peroxidation (LPO) and decreases the growth rate of mammary and other tumors. However, the results of an earlier study indicated that a factor in addition to LPO was involved in the reduction in tumor growth in n-3 PUFAs fed mice. Athymic mice bearing MDA-MB-231 human breast carcinoma xenografts, were fed fish oil concentrate (FOC) or control diets, with and without supplemental Vitamin E (2000 IU /kg diet) and were sacrificed both before and after doxorubicin (DOX) treatment to evaluate factors involved in tumor growth suppression. Results: Prior to DOX, basal LPO in the tumor of 3% FOC fed mice was slightly higher than in the control fed mice and was decreased in mice consuming FOC with vitamin E. Vitamin E suppressed the DOX induced increase in LPO in the tumors of control mice, however, vitamin E was not sufficient to suppress a DOX induced increase in LPO in the tumors of FOC fed mice. The mean growth rate of tumors of FOC fed mice was significantly less than the mean growth rate of the tumors of control mice. Multiple regression analyses indicated that suppression of glutathione peroxidase (GPX) activity by FOC prior to DOX therapy was more important than increased LPO as an explanation of tumor growth suppression. Tumor induced cachexia was decreased in mice consuming FOC. Conclusions: It appears that the increased sensitivity to DOX was related to an FOC induced reduction in GPX activity. FOC reduced tumor induced cachexia

Perancangan Buku Batik Tulis Jarak Arum Ex-lokalisasi Dolly dengan Teknik Watercolor Guna Meningkatkan Brand Awareness

Batik tulis Jarak Arum is one of the unique batik Surabaya from ex-Dolly. Jarak Arum\u27s batik has its own characteristics which is illustrated by the motifs, where the motive is to tell about the ex-Dolly localization earlier known as the second largest in Asia. Through motives contained in batik is expected to lift the image of Dolly earlier known as localization region the second largest in Asia which has now been folded Dolly now exists in name only. With this in mind to improve the image of the previous Dolly to become one of the tourist areas in Surabaya which is famous for its batik is batik tulis Jarak Arum, is expected to change the image of Dolly the better. Batik tulis Jarak Arum also including new products that require media for recognition, in order to be able to become one of the products that can be remembered and recognized by the public. Therefore, this research aims to design book of batik tulis Jarak Arum ex-Dolly with watercolor techniques in order to increase brand awareness. The design of the book batik tulis Jarak Arum ex-Dolly using qualitative data collection methods, namely by interviews, observation, literature study, existing studies and creative brief is very important to define the concept design study. From the analysis of these data, the theme of the design concept obtained is charming. Concepts Charming aims to communicate to the target market, namely the premature adults who are less familiar and interested to be more familiar and batik and recall batik tulis Jarak Arum as part of a product category, with emphasis on product charming character