24 research outputs found
Applicability of the 5S management method for quality improvement in health-care facilities: a review
Electric power supply and demand 1979 to 1988 for the contiguous United States as projected by the Regional Electric Reliability Councils in their April 1, 1979 long-range coordinated planning reports to the Department of Energy
Information concerning bulk electric power supply and demand is summarized and reviewed. Electric-utility power-supply systems are composed of power sources, transmission and distribution facilities, and users of electricity. In the United States there are three such systems of large geographic extent that together cover the entire country. Subjects covered are: energy forecasts, peak demand forecasts, generating-capacity forecasts, purchases and sales of capacity, and transmission. Extensive data are compiled in 17 tables. Information in two appendices includes a general description of the Regional Electric Reliability Councils and US generating capacity as of June 30, 1979. 3 figures, 17 tables
Thermophysical properties and elemental composition of agricultural and forest solid biofuels versus fossil fuels
Investigating Therapeutic Protein Structure with Diethylpyrocarbonate Labeling and Mass Spectrometry
Protein therapeutics are rapidly
transforming the pharmaceutical
industry. Unlike for small molecule therapeutics, current technologies
are challenged to provide the rapid, high-resolution analyses of protein
higher order structures needed to ensure drug efficacy and safety.
Consequently, significant attention has turned to developing new methods
that can quickly, accurately, and reproducibly characterize the three-dimensional
structure of protein therapeutics. In this work, we describe a method
that uses diethylpyrocarbonate (DEPC) labeling and mass spectrometry
to detect three-dimensional structural changes in therapeutic proteins
that have been exposed to degrading conditions. Using β2-microglobulin,
immunoglobulin G1, and human growth hormone as model systems, we demonstrate
that DEPC labeling can identify both specific protein regions that
mediate aggregation and those regions that undergo more subtle structural
changes upon mishandling of these proteins. Importantly, DEPC labeling
is able to provide information for up to 30% of the surface residues
in a given protein, thereby providing excellent structural resolution.
Given the simplicity of the DEPC labeling chemistry and the relatively
straightforward mass spectral analysis of DEPC-labeled proteins, we
expect this method should be amenable to a wide range of protein therapeutics
and their different formulations