Model calibration against CB1Ra data.

<p>The calibrated model shows good agreement between model simulations (black) and experimental BW (A). The fitted model trajectories show poor agreement with FFM (B) and FM (C) measurements at the three drug dose levels. Gray region indicates the intervention phase in each study. Error bars represent SEM (9-10 rats).</p

The α-free model fits BC in CR and CB1Ra BC studies.

<p>Simulations (black) of the <i>α</i>-free model show agreement with FFM (red) and FM (yellow) measurements in CR (A) and CB1Ra (B) intervention studies. Gray region indicates treatment phases in the two studies. Error bars represent SEM (9-10 rats).</p

Body composition data for CR and CB1Ra treatment studies.

<p>Different color curves show changes in body composition in response to different levels of CR (A) and CB1Ra (B). Changes in FFM and FM in (A) and (B) do not appear along a single pre-defined FFM-FM curve (dashed line) defined by the energy partition function [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155674#pone.0155674.ref007" target="_blank">7</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155674#pone.0155674.ref008" target="_blank">8</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0155674#pone.0155674.ref021" target="_blank">21</a>]. The different shapes correspond to three time points when BC measurements were taken. The colored line segments connecting data points are meant to guide the reader’s eye. Error bars represent SEM (9-10 rats).</p

The α-free model allows for estimation of body composition changes compared to the two-dimensional model.

<p>Body composition simulations of the <i>α</i>-free model (blue) can be used to estimate FM and FFM outside of measured time points, black. The two-dimensional model simulation based on the <i>α</i> function (red) is unable to accurately capture changes in FM and FFM (purple). Only data for the 30 mg/kg dose is shown, with the purple line segments meant to guide the reader’s eye. Error bars represent SEM (9-10 rats).</p

Model calibration against CR data.

<p>The calibrated model shows good agreement between model simulations (black) and experimental BW (A), FFM (B), and FM (C) measurements at all caloric restriction levels. Gray region indicates the intervention phase in each study. Error bars represent SEM (9-10 rats).</p