Levels of Ycg1 Limit Condensin Function during the Cell Cycle

During mitosis chromosomes are condensed to facilitate their segregation, through a process mediated by the condensin complex. Although several factors that promote maximal condensin activity during mitosis have been identified, the mechanisms that downregulate condensin activity during interphase are largely unknown. Here, we demonstrate that Ycg1, the Cap-G subunit of budding yeast condensin, is cell cycle-regulated with levels peaking in mitosis and decreasing as cells enter G1 phase. This cyclical expression pattern is established by a combination of cell cycle-regulated transcription and constitutive degradation. Interestingly, overexpression of YCG1 and mutations that stabilize Ycg1 each result in delayed cell-cycle entry and an overall proliferation defect. Overexpression of no other condensin subunit impacts the cell cycle, suggesting that Ycg1 is limiting for condensin complex formation. Consistent with this possibility, we find that levels of intact condensin complex are reduced in G1 phase compared to mitosis, and that increased Ycg1 expression leads to increases in both levels of condensin complex and binding to chromatin in G1. Together, these results demonstrate that Ycg1 levels limit condensin function in interphase cells, and suggest that the association of condensin with chromosomes must be reduced following mitosis to enable efficient progression through the cell cycle

Toll-like Receptor-4 Regulation of Hepatic Cyp3a11 Metabolism in a Mouse Model of LPS-induced CNS Inflammation

Central nervous system (CNS) infection and inflammation severely reduce the capacity of cytochrome P-450 metabolism in the liver. We developed a mouse model to examine the effects of CNS inflammation on hepatic cytochrome P-450 metabolism. FVB, C57BL/6, and C3H/HeouJ mice were given Escherichia coli LPS (2.5 μg) by intracerebroventricular (ICV) injection. The CNS inflammatory response was confirmed by the elevation of TNF-α and/or IL-1β proteins in the brain. In all mouse strains, LPS produced a 60–70% loss in hepatic Cyp3a11 expression and activity compared with saline-injected controls. Adrenalectomy did not prevent the loss in Cyp3a11 expression or activity, thereby precluding the involvement of the hypothalamic-adrenal-pituitary axis. Endotoxin was detectable (1–10 ng/ml) in serum between 15 and 120 min after ICV dosing of 2.5 μg LPS. Peripheral administration of 2.5 μg LPS by intraperitoneal injection produced similar serum endotoxin levels and a similar loss (60%) in Cyp3a11 expression and activity in the liver. The loss of Cyp3a11 in response to centrally or peripherally administered LPS could not be evoked in Toll-like receptor-4 (TLR4)-mutant (C3H/ HeJ) mice, indicating that TLR4 signaling pathways are directly involved in the enzyme loss. In summary, we conclude that LPS is transferred from the brain to the circulation in significant quantities in a model of CNS infection or inflammation. Subsequently, LPS that has reached the circulation stimulates a TLR4-dependent mechanism in the periphery, evoking a reduction in Cyp3a11 expression and metabolism in the liver

A small plot potato planter

A small plot potato planter. Can. Agric. Eng. 38: 145-147. A two-row planter was constructed for planting small potato research plots. For each row, seed pieces were manually placed on a cup conveyor belt system. Desired in-row seed piece spacings, per row, were obtained by controlling the speed of the conveyor belts using variable speed gearboxes. Micro-dial controllers, attached to the gearboxes, allowed for rapid adjustment of in-row seed piece spacing when required. Small fertilizer hoppers, similar in design to commercial planters, were attached to the planter when a uniform type or rate of fertilizer was required at planting. When planting required different types or rates of fertilizer, hoppers were removed and replaced by two Hege (model H80) belt cones. Fertilizer required per treatment row was placed in the cone prior to planting then applied as cones rotated. The rotation of the cones was controlled by a variable speed gearbox with micro-dial control to adjust the row length of fertilizer application when required. The planter performed well compared to commercial and other small plot planters. The coefficient of variation for seed piece spacings ranged between II to 15% and labour required for planting was 40 to 60% less compared to hand planting

The Fueling of Nuclear Activity: II. The Bar Properties of Seyfert and Normal Galaxies

We use a recent near-infrared imaging survey of samples of Seyfert and normal galaxies to study the role of bars in the fueling of nuclear activity. The active galaxy sample includes Seyfert galaxies in the Revised Shapely-Ames (RSA) and Sandage & Tammann's (1987) extension to this catalog. The normal galaxies were selected to match the Seyfert sample in Hubble type, redshift, inclination and blue luminosity. All the galaxies in both samples classified as barred in the RSA catalog are also barred in the near-infrared. In addition, ~55% of the galaxies classified as non-barred in the RSA show evidence for bars at 2.1 microns. Overall, ~70% of the galaxies observed show evidence for bar structures. The incidence of bars in the Seyfert and normal galaxies is similar, suggesting Seyfert nuclei do not occur preferentially in barred systems. Furthermore, a slightly higher percentage of normal galaxies have multiple-bar structures.Comment: aastex 4.0, accepted for publication in the Astrophysical Journal Letter

The Influence of Bars on Nuclear Activity

We test ideas on fueling of galactic nuclei by bar-driven inflow by comparing the detection rate and intensity of nuclear H II regions and AGNs among barred and unbarred galaxies in a sample of over 300 spirals selected from our recent optical spectroscopic survey of nearby galaxies. Among late-type spirals (Sc-Sm), but not early-type (S0/a-Sbc), we observe in the barred group a very marginal increase in the detection rate of H II nuclei and a corresponding decrease in the incidence of AGNs. The minor differences in the detection rates, however, are statistically insignificant, most likely stemming from selection effects and not from a genuine influence from the bar. The presence of a bar seems to have no noticeable impact on the likelihood of a galaxy to host either nuclear star formation or an AGN. The nuclei of early-type barred spirals do exhibit measurably higher star-formation rates than their unbarred counterparts, as indicated by either the luminosity or the equivalent width of H-alpha emission. By contrast, late-type spirals do not show such an effect. Bars have a negligible effect on the strength of the AGNs in our sample, regardless of the Hubble type of the host galaxy. This result confirms similar conclusions reached by other studies based on much smaller samples.Comment: To appear in the Astrophysical Journal. LaTex, 31 pages including 6 postscript figures and 3 tables. AAStex macros include

The Disks of Galaxies with Seyfert and Starburst Nuclei: II. Near-Infrared Structural Properties

We have derived the near-infrared structural components of a sample of Seyfert and starburst (SBN) host galaxies by fitting the images of Hunt et al. (1997,ApJS,108,229) with a new two-dimensional decomposition algorithm. An analysis of the fitted parameters shows that Seyfert 1 and SBN bulges resemble normal early-type bulges in structure and color, with (J-K)^c_b about 0.1 mag redder than disk (J-K)^c_d. Seyfert 2 bulges, instead, are bluer than normal with (J-K)^c_b ~ (J-K)^c_d. Seyfert disks (especially Type 1), but not those of SBNs, are abnormally bright (in surface brightness), significantly more so than even the brightest normal disks. Seyfert disks are also compact, but similar to those in normal early-type spirals. For a given mass, Seyferts and especially SBNs are abnormally rich in neutral hydrogen, and there is strong, albeit indirect, evidence for lower mass-to-light (M/L) ratios in Seyfert and SBN disks, but for normal M/Ls in their bulges. In Seyferts and SBNs, HI mass fractions and M/L ratios are anticorrelated, and we attribute the high gas mass fractions and low M/Ls in SBNs and several Seyferts to ongoing star formation. Such abundant gas in Seyferts would be expected to inhibit bar formation, which may explain why active galaxies are not always barred.Comment: 25 pgs (two-column, single-spaced) including 8 incorporated figures and 2 tables (aas2pp4, amssym, epsfig). Accepted for publication in Ap