34 research outputs found
Soil Dehydrogenases as an Indicator of Contamination of the Environment with Petroleum Products
Soil phosphorus supply controls P nutrition strategies of beech forest ecosystems in Central Europe
Soil Acidification under Tea Bushes and Its Influence on the Biological Characteristics of a Red Soil
Transfusion / Glycated hemoglobin concentrations of packed red blood cells minimally increase during storage under standard blood banking conditions
BACKGROUND
Few and inconsistent data exist describing the effect of storage duration on glycated hemoglobin (HbA1c) concentrations of red blood cells (RBCs), impeding interpretation of HbA1c values in transfused diabetic patients. Hence the aim of this study was to evaluate to what extent HbA1c concentrations of RBCs change during the maximum allowed storage period of 42 days.
STUDY DESIGN AND METHODS
Blood was drawn from 16 volunteers, leukofiltered, and stored under standard blood banking conditions. HbA1c concentrations of RBCs were measured on Days 1 and 42 of storage using three different validated devices (ionexchange highperformance liquid chromatography Method A1 and A2, turbidimetric immunoassay Method B).
RESULTS
Mean HbA1c concentrations of RBCs on Day 1 were 5.3 0.3% (Method A1), 5.4 0.4% (Method A2), and 5.1 0.4% (Method B). HbA1c concentrations increased to 5.6 0.3% (A1, p < 0.0001), 5.7 0.3% (A2, p = 0.004), and 5.5 0.4% (B, p < 0.0001) on Day 42, respectively, corresponding to a 1.06fold increase across all methods. Glucose concentrations in the storage solution of RBCs decreased from 495 27 to 225 55 mg/dL (p < 0.0001), confirming that stored RBCs were metabolically active.
CONCLUSION
These results suggest a significant, albeit minor, and most likely clinically insignificant increase in HbA1c concentrations during storage of RBCs for 42 days.(VLID)341760