Antagonistic Roles of SEPALLATA3, FT and FLC Genes as Targets of the Polycomb Group Gene CURLY LEAF

In Arabidopsis, mutations in the Pc-G gene CURLY LEAF (CLF) give early flowering plants with curled leaves. This phenotype is caused by mis-expression of the floral homeotic gene AGAMOUS (AG) in leaves, so that ag mutations largely suppress the clf phenotype. Here, we identify three mutations that suppress clf despite maintaining high AG expression. We show that the suppressors correspond to mutations in FPA and FT, two genes promoting flowering, and in SEPALLATA3 (SEP3) which encodes a co-factor for AG protein. The suppression of the clf phenotype is correlated with low SEP3 expression in all case and reveals that SEP3 has a role in promoting flowering in addition to its role in controlling floral organ identity. Genetic analysis of clf ft mutants indicates that CLF promotes flowering by reducing expression of FLC, a repressor of flowering. We conclude that SEP3 is the key target mediating the clf phenotype, and that the antagonistic effects of CLF target genes masks a role for CLF in promoting flowering

Matrix relations in the analysis of variance

Artykuł nie zawiera streszczeniaFor balanced classifications and for unbalanced nested classifications, we give a simple rule which establishes a one-to-one correspondence between numbers of degrees of freedom and projection operators in the analysis of variance. The rule enables us to determine explicitly the matrix representations of appropriate sums of squares

Przegląd niektórych prac Katedry Zastosowań Matematyki Akademii Rolniczej w Lublinie

For balanced classifications and for unbalanced nested classifications, we give a simple rule which establishes a one-to-one correspondence between numbers of degrees of freedom and projection operators in the analysis of variance. The rule enables us to determine explicitly the matrix representations of appropriate sums of squares