2,121 research outputs found
Effects of Elevated H\u3csup\u3e+\u3c/sup\u3e And P\u3csub\u3ei\u3c/sub\u3e on The Contractile Mechanics of Skeletal Muscle Fibres From Young and Old Men: Implications for Muscle Fatigue in Humans
The present study aimed to identify the mechanisms responsible for the loss in muscle power and increased fatigability with ageing by integrating measures of wholeâmuscle function with single fibre contractile mechanics. After adjusting for the 22% smaller muscle mass in old (73â89 years, n = 6) compared to young men (20â29 years, n = 6), isometric torque and power output of the knee extensors were, respectively, 38% and 53% lower with age. Fatigability was âŒ2.7âfold greater with age and strongly associated with reductions in the electricallyâevoked contractile properties. To test whether crossâbridge mechanisms could explain ageârelated decrements in knee extensor function, we exposed myofibres (n = 254) from the vastus lateralis to conditions mimicking quiescent muscle and fatiguing levels of acidosis (H+) (pH 6.2) and inorganic phosphate (Pi) (30 mm). The fatigueâmimicking condition caused marked reductions in force, shortening velocity and power and inhibited the lowâ to highâforce state of the crossâbridge cycle, confirming findings from nonâhuman studies that these ions act synergistically to impair crossâbridge function. Other than severe ageârelated atrophy of fast fibres (â55%), contractile function and the depressive effects of the fatigueâmimicking condition did not differ in fibres from young and old men. The selective loss of fast myosin heavy chain II muscle was strongly associated with the ageârelated decrease in isometric torque (r = 0.785) and power (r = 0.861). These data suggest that the ageârelated loss in muscle strength and power are primarily determined by the atrophy of fast fibres, but the ageârelated increased fatigability cannot be explained by an increased sensitivity of the crossâbridge to H+ and Pi
Using RNA-based therapies to target the kidney in cardiovascular disease
RNA-based therapies are currently used for immunisation against infections and to treat metabolic diseases. They can modulate gene expression in immune cells and hepatocytes, but their use in other cell types has been limited by an inability to selectively target specific tissues. Potential solutions to this targeting problem involve packaging therapeutic RNA molecules into delivery vehicles that are preferentially delivered to cells of interest. In this review, we consider why the kidney is a desirable target for RNA-based therapies in cardiovascular disease and discuss how such therapy could be delivered. Because the kidney plays a central role in maintaining cardiovascular homeostasis, many extant drugs used for preventing cardiovascular disease act predominantly on renal tubular cells. Moreover, kidney disease is a major independent risk factor for cardiovascular disease and a global health problem. Chronic kidney disease is projected to become the fifth leading cause of death by 2040, with around half of affected individuals dying from cardiovascular disease. The most promising strategies for delivering therapeutic RNA selectively to kidney cells make use of synthetic polymers and engineered extracellular vesicles to deliver an RNA cargo. Future research should focus on establishing the safety of these novel delivery platforms in humans, on developing palatable routes of administration and on prioritising the gene targets that are likely to have the biggest impact in cardiovascular disease
Computer Storage and Retrieval System for Two-Dimensional Outlines
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/67260/2/10.1177_00220345700490053201.pd
NMFS / Interagency Working Group Evaluation of CITES Criteria and Guidelines.
EXECUTIVE SUMMARY: At present, the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) criteria used to assess whether a population qualifies for inclusion in the CITES Appendices relate to (A) size of the population, (B) area of distribution of the population, and (C) declines in the size of the population. Numeric guidelines are provided as indicators of a small population (less than 5,000 individuals), a small subpopulation (less than 500 individuals), a restricted area of distribution for a population (less than 10,000 km2), a restricted area of distribution for a subpopula-tion (less than 500 km2), a high rate of decline (a decrease of 50% or more in total within 5 years or two generations whichever is longer or, for a small wild population, a decline of 20% or more in total within ten years or three generations whichever is longer), large fluctuations (population size or area of distribution varies widely, rapidly and frequently, with a variation greater than one order of magnitude), and a short-term fluctuation (one of two years or less).
The Working Group discussed several broad issues of relevance to the CITES criteria and guidelines. These included the importance of the historical extent of decline versus the recent rate of decline; the utility and validity of incorporating relative population productivity into decline criteria; the utility of absolute numbers for defining small populations or small areas; the appropriateness of generation times as time frames for examining declines; the importance of the magnitude and frequency of fluctuations as factors affecting risk of extinction; and the overall utility of numeric thresh-olds or guidelines
Interpersonal prosodic correlation in frontotemporal dementia.
Communication accommodation describes how individuals adjust their communicative style to that of their conversational partner. We predicted that interpersonal prosodic correlation related to pitch and timing would be decreased in behavioral variant frontotemporal dementia (bvFTD). We predicted that the interpersonal correlation in a timing measure and a pitch measure would be increased in right temporal FTD (rtFTD) due to sparing of the neural substrate for speech timing and pitch modulation but loss of social semantics. We found no significant effects in bvFTD, but conversations including rtFTD demonstrated higher interpersonal correlations in speech rate than healthy controls
The Star Clusters in the Starburst Irregular Galaxy NGC 1569
We examine star clusters in the irregular, starburst galaxy NGC 1569 from HST
images. In addition to the two known super star clusters, we identify 45 other
clusters that are compact but resolved. Integrated UVI colors of the clusters
span a large range, and suggest that ages range from 3 Myrs to 1 Gyr. However,
most of the clusters were formed at the tail end of the recent starburst.
Numerous clusters in addition to the know super star clusters are similar in
luminosity to a small globular cluster. We examined the radial surface
brightness of four of the clusters. Their half-light radii and core radii are
in the range observed in present-day globular clusters. Therefore, conditions
that produced the recent starburst have also been those necessary for producing
compact, bright star clusters.
We examine resolved stars in the outer parts of the two super star clusters.
Cluster A is dominated by bright blue stars with a small population of red
supergiants. Sub-components A1 and A2 have similar colors and a two-dimensional
color map does not offer evidence that one component is dominated by red
supergiants and the other not. The contradiction of the presence of red super-
giants with Wolf-Rayet stars may instead not be a contradiction at all since
there coexistence in a coeval population is not inconsistent with the evolution
of massive stars. Cluster B is dominated by red supergiants, and this is
confirmed by the presence of the stellar CO absorption feature in an integrated
spectrum. The various age indicators are consistent with a picture in which
cluster B is of order 10--20 Myrs old, and cluster A is >4-5 Myrs old.Comment: To be published in AJ, November 200
- âŠ