124 research outputs found
Use of the enzyme hexokinase for the reduction of inherent light levels
Use of enzyme hexokinase and glucose to reduce inherent light levels of ATP in luciferase composition
Method of detecting and counting bacteria in body fluids
A novel method is reported for determining bacterial levels in urine samples, which method depends on the quantitative determination of bacterial adenosine triphosphate (ATP) in the presence of non-bacterial ATP. After the removal of non-bacterial ATP, the bacterial ATP is released by cell rupture and is measured by an enzymatic bioluminescent assay using an enzyme obtained from the firefly
Bacterial adenosine triphosphate as a measure of urinary tract infection
Procedure detects and counts bacteria present in urine samples. Method also determines bacterial levels in other aqueous body fluids including lymph fluid, plasma, blood, spinal fluid, saliva and mucous
Method of detecting and counting bacteria
An improved method is provided for determining bacterial levels, especially in samples of aqueous physiological fluids. The method depends on the quantitative determination of bacterial adenosine triphosphate (ATP) in the presence of nonbacterial ATP. The bacterial ATP is released by cell rupture and is measured by an enzymatic bioluminescent assay. A concentration technique is included to make the method more sensitive. It is particularly useful where the fluid to be measured contains an unknown or low bacteria count
Light detection instrument Patent
Detection instrument for light emitted from ATP biochemical reactio
Quantitive determination of flavin nucleotide using the bacterial bioluminescent reaction
Photometric method based on the use of bacterial luminiscent reaction quantitatively detects the presence of flavin compounds in all forms of life. Aqueous cellular dispersion of a biological sample with an aqueous perchloric acid ruptures the cells and frees the flavin coenzymes from their proteins
Protein sterilization method of firefly luciferase using reduced pressure and molecular sieves
The sterilization of the protein fruitfly luciferase under conditions that prevent denaturation is examined. Denaturation is prevented by heating the protein in contact with molecular seives and under a reduced pressure of the order of 0.00005 millimeters of mercury
Adenosine triphosphate (ATP) as a possible indicator of extraterrestrial biology
The ubiquity of adenosine triphosphate (ATP) in terrestrial organisms provides the basis for proposing the assay of this vital metabolic intermediate for detecting extraterrestrial biological activity. If an organic carbon chemistry is present on the planets, the occurrence of ATP is possible either from biosynthetic or purely chemical reactions. However, ATP's relative complexity minimizes the probability of abiogenic synthesis. A sensitive technique for the quantitative detection of ATP was developed using the firefly bioluminescent reaction. The procedure was used successfully for the determination of the ATP content of soil and bacteria. This technique is also being investigated from the standpoint of its application in clinical medicine
Analytical Applications of Bioluminescence and Chemiluminescence
Bioluminescence and chemiluminescence studies were used to measure the amount of adenosine triphosphate and therefore the amount of energy available. Firefly luciferase - luciferin enzyme system was emphasized. Photometer designs are also considered
Mass culture of photobacteria to obtain luciferase
Inoculating preheated trays containing nutrient agar with photobacteria provides a means for mass culture of aerobic microorganisms in order to obtain large quantities of luciferase. To determine optimum harvest time, growth can be monitored by automated light-detection instrumentation
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