Tahikardija u novorođenčeta s enterovirusnom infekcijom

Enterovirus infections are common in the neonatal period. Newborns are at a higher risk of severe disease including meningoencephalitis, sepsis syndrome, cardiovascular collapse, or hepatitis. The mechanism of heart failure in patients with enterovirus infection remains unknown. Early diagnosis may help clinicians predict complications in those infants initially presenting with severe disease. An 11-day-old male newborn was admitted to our neonatal intensive care unit because of tachycardia and crises of cyanosis. His elder brother had febrile illness. The newborn was cyanotic, in respiratory distress, with tachycardia, low blood pressure and prolonged capillary refilling time. Limb pulse oximeter was around 85%. During the first day of hospitalization, the newborn had one febrile episode. Laboratory data: elevated transaminases, markers of inflammation negative, all bacterial cultures negative. Enterovirus RNA was detected in blood sample. Other blood findings were without significant abnormalities. Electrocardiogram showed tachycardia, with narrow QRS complexes (atrial tachycardia) and heart rate up to 280/min. In order to convert the rhythm, the patient was administered adenosine and amiodarone. In the further course of hospitalization, the patient was in good general condition, eucardiac and eupneic. Newborns with tachycardia and a family history of febrile illness should be suspected to have enterovirus infection. Enterovirus infection is a highly contagious and potentially life-threatening infection if not detected early. The use of sensitive molecular-based amplification methods offers potential benefits for early diagnosis and timely treatment.Enterovirusne infekcije su uobičajene u neonatalnom razdoblju. Novorođenčad su pod većim rizikom za teške oblike bolesti, uključujući meningoencefalitis, sindrom sepse, kardiovaskularni kolaps ili hepatitis. Mehanizam zatajenja srca u bolesnika s enterovirusnim infekcijama ostaje nepoznat. Rana dijagnoza može pomoći kliničarima da predvide komplikacije na početku u dojenčadi s teškom bolešću. Muško novorođenče staro 11 dana primljeno je u našu jedinicu intenzivne neonatalne skrbi zbog tahikardije i krize cijanoze. Njegov stariji brat imao je febrilnu bolest. Novorođenče je bilo cijanotično, u respiracijskom distresu, uz tahikardiju, nizak krvni tlak i produženo kapilarno punjenje. Pulsna oksimetrija je bila oko 85%. Tijekom prvog dana hospitalizacije novorođenče je imalo jednu febrilnu epizodu. Laboratorijski nalazi su pokazali povišene transaminaze, negativne parametre upale i negativne sve bakterijske kulture. Enterovirusna RNA je otkrivena u uzorku krvi. Ostali nalazi krvi bili su bez značajnih odstupanja. Elektrokardiogram je pokazao tahikardiju s uskim kompleksima QRS (atrijska tahikardija) i srčanom frekvencijom do 280/min. Radi pretvorbe ritma bolesnik je dobio adenozin i amjodaron. U daljnjem tijeku hospitalizacije bolesnik je bio dobrog općeg stanja, eukardičan i eupnoičan. Kod novorođenčadi s tahikardijom i podacima o febrilnosti kod drugih članova obitelji treba posumnjati na enterovirusnu infekciju. Enterovirusna infekcija je vrlo zarazna i potencijalno opasna za život ako se ne otkrije na vrijeme. Osjetljive molekularne metode nude potencijalne prednosti za rano otkrivanje i pravodobno liječenje

Human Papillomavirus Genotype Spectrum in Studied Group of Montenegrin Women

The risk of high-grade lesions and carcinoma is known to correlate with specific high-risk HPV genotypes. The distribution of HPV types varies between countries and little is known about HPV distribution in our country. Therefore, the purpose of this study was to determine the range and frequency of HPV genotypes in studied group of women in Montenegro. HPV genotypes were determined using the method of enzyme restriction of PCR products amplified with group-specific primers MY09/MY11 and restricted with seven different restriction endonucleases. Out of the total number of women HPV infection was found in 1/5 of participants (20%). Genotyping performed in HPV DNA positive women shows that the HPV genotype 16 is dominant and present in more than 1/3 of the participants (36.8%). The second most frequent HPV infection is with HPV genotype 58 and it is found in 10.5% of participants. HPV 31 and HPV 6 infections are present in 7.9% of women, while infections with other genotypes were demonstrated individually by 2.6%. Multiple HPV infection was demonstrated in 18.4% and they are dominant in younger women (aged 25 to 30 years)