Serum IgA, IgG, IgM and Salivary IgA in Recurrent Aphthous Ulceration

Radial immunodiffusion technique was used to estimate salivary immunoglobulin A, and enzyme-linked immunosorbent assay for estimation of serum IgA, IgG and IgM in 30 patients with acute recurrent aphthous ulceration (RAU) and during remission period compared to 30 healthy controls. Significantly elevated level of salivary IgA (p < 0.05) was found in patients with minor RAU when compared to the control group. Serum IgA level was elevated in patients with minor acute RAU when compared to the controls (p < 0.05). Serum immunoglobulin level of IgG and IgM showed no differences between patients with either minor or major recurrent aphthous ulceration and controls

Burning mouth syndrome as the initial sign of multiple myeloma

AbstractA 79-year-old women was referred to our Department with burning symptoms in the whole oral cavity together with clinically healthy appearence of the oral mucosa. Hematological tests as well as bone marrow biopsy revealed diagnosis of plasmocytoma. The aim of our case report was to underline the importance of hematological screening in patients with burning mouth syndrome

The Prevalence of Candida Albicans in Institutionalized Geriatric Denture Wearers

The aim of this prospective study was to record the presence of Candida albicans yeast in a geriatric population, and to compare the results between denture wearers and non-wearers, in order to evaluate the role of dentures as a factor influencing Candida growth. We performed an oral examination on 217 institutionalized elderly people living in the hospice "Sv. Josip" in Zagreb, recorded data regarding oral health and collected detailed information on denture wearing status. Furthermore, we performed oral smears for Candida cultures, cultivated on Sabouraud\u27s dextrose agar (“Emmon\u27s”) at 37°C for 48 hrs and counted the Candida colonies. Collected data were stored in the database created for this purpose in "Microsoft Access 2000". The number of Candida colonies from denture wearers and non-wearers were compared (Man-Whitney U test, p<0.05), and the obtained results are discussed

Salivary IgA and IgG Subclass Levels in Patients with Oral Lichen Planus - A Pilot Study

Oralni lihen planus (OLP) kronična je upalna bolest koju karakterizira imunoreaktivnost usmjerena prema bazalnim keratinocitima i posredovana je T-limfocitima. Svrha istraživanja bila je odrediti potklase salivarnog imunoglobulina A1 (IgA1) i IgA2 te IgG1,2,3,4 kod bolesnika s retikularnim oblikom OLP-a tijekom akutne faze i remisije te u kontrolnoj skupini. U ukupnoj nestimuliranoj slini kod 19 bolesnika s OLP-om u dobi od 30 do 72 godine, srednje dobi od 58 godina, u akutnoj fazi i tijekom remisije te kod 21 kontrolnog ispitanika u dobi od 20 do 52 godine, srednje dobi od 35 godina, određene su potklase salivarnog IgA-a radijalnom imunodifuzijom i potklase salivarnog IgG-a uz pomoć enzimskog imunotesta. Između bolesnika u akutnoj fazi i kontrolnih ispitanika nije bilo znatnih razlika u IgG-u1 i 2 IgA-a1 te IgA-a2 (p>0,05). Bolesnici u akutnoj fazi imali su znatno veće vrijednosti IgG-a3 i IgG-a4 te proteina sline (p=0,021; p=0,004; p=0,029) u odnosu prema kontrolnoj skupini. Između bolesnika u akutnoj fazi i u remisiji nije bilo većih razlika u vrijednostima IgG-a1,2,3,4 i IgA-a1, a znatno su bile povišene vrijednosti IgA-a2 ustanovljene u akutnoj fazi (p=0,049). Između bolesnika u fazi remisije i kontrolne skupine nije bilo razlika ni u jednoj salivarnoj potklasi - bilo IgA-a bilo IgG-a (p>0,05). Iz svega navedenoga može se zaključiti da u akutnoj fazi raste IgA2, što bi mogao biti utjecaj pojačane aktivnosti sekretorne imunosti, a možda je i posljedica mikrobne stimulacije koja se vidi u akutnoj fazi lihena u odnosu prema fazi remisije.Oral lichen planus (OLP) is a chronic inflammatory disease which is characterized by an immunoreactivity directed against basal keratinocytes and mediated by T/lymphocytes. However, it is well known that salivary immunoglobulins have important role in the protection of mucosal surfaces. The aim of this study was to determine salivary immunoglobulin A1 (IgA1) and IgA2, together with IgG 1,2,3,4 subclass levels in patients with oral reticular lichen planus during acute stage and remission period as well as in comparison to the controls. In the whole resting saliva of 19 patients with OLP, age range 30-72, mean 58 years in acute phase and during remission period, and in 21 controls, age range 20-52, mean 35 years, salivary IgA and IgG subclasses were determined with radial immunodiffusion and enzyme immunoassay respectively. There were no significant differences in salivary IgG1 and IgG2 as well as IgA1 and IgA2 between patients in acute phase and controls (p>0.05). Patients in acute phase had significantly increased IgG3, IgG4 and proteins in comparison to the controls (p=0,021; p=0,004; p=0,029). No significant differences could be found between patients in acute phase and during remission period in IgG1,2,3,4 and IgA1 while IgA2 was significantly increased in acute phase in comparison to the remission period (p=0,049). Between patients in remission period and controls there were no significant differences in any IgA or IgG salivary subclasses (p>0,05). We can conclude that acute phase is characterized with increase in IgA2 which might reflect increased activity of secretory immunity as a possible result of microbial stimulation seen in acute phase in comparison to the remission period

Salivary IgA and IgG Subclass Levels in Patients with Oral Lichen Planus - A Pilot Study

Oralni lihen planus (OLP) kronična je upalna bolest koju karakterizira imunoreaktivnost usmjerena prema bazalnim keratinocitima i posredovana je T-limfocitima. Svrha istraživanja bila je odrediti potklase salivarnog imunoglobulina A1 (IgA1) i IgA2 te IgG1,2,3,4 kod bolesnika s retikularnim oblikom OLP-a tijekom akutne faze i remisije te u kontrolnoj skupini. U ukupnoj nestimuliranoj slini kod 19 bolesnika s OLP-om u dobi od 30 do 72 godine, srednje dobi od 58 godina, u akutnoj fazi i tijekom remisije te kod 21 kontrolnog ispitanika u dobi od 20 do 52 godine, srednje dobi od 35 godina, određene su potklase salivarnog IgA-a radijalnom imunodifuzijom i potklase salivarnog IgG-a uz pomoć enzimskog imunotesta. Između bolesnika u akutnoj fazi i kontrolnih ispitanika nije bilo znatnih razlika u IgG-u1 i 2 IgA-a1 te IgA-a2 (p>0,05). Bolesnici u akutnoj fazi imali su znatno veće vrijednosti IgG-a3 i IgG-a4 te proteina sline (p=0,021; p=0,004; p=0,029) u odnosu prema kontrolnoj skupini. Između bolesnika u akutnoj fazi i u remisiji nije bilo većih razlika u vrijednostima IgG-a1,2,3,4 i IgA-a1, a znatno su bile povišene vrijednosti IgA-a2 ustanovljene u akutnoj fazi (p=0,049). Između bolesnika u fazi remisije i kontrolne skupine nije bilo razlika ni u jednoj salivarnoj potklasi - bilo IgA-a bilo IgG-a (p>0,05). Iz svega navedenoga može se zaključiti da u akutnoj fazi raste IgA2, što bi mogao biti utjecaj pojačane aktivnosti sekretorne imunosti, a možda je i posljedica mikrobne stimulacije koja se vidi u akutnoj fazi lihena u odnosu prema fazi remisije.Oral lichen planus (OLP) is a chronic inflammatory disease which is characterized by an immunoreactivity directed against basal keratinocytes and mediated by T/lymphocytes. However, it is well known that salivary immunoglobulins have important role in the protection of mucosal surfaces. The aim of this study was to determine salivary immunoglobulin A1 (IgA1) and IgA2, together with IgG 1,2,3,4 subclass levels in patients with oral reticular lichen planus during acute stage and remission period as well as in comparison to the controls. In the whole resting saliva of 19 patients with OLP, age range 30-72, mean 58 years in acute phase and during remission period, and in 21 controls, age range 20-52, mean 35 years, salivary IgA and IgG subclasses were determined with radial immunodiffusion and enzyme immunoassay respectively. There were no significant differences in salivary IgG1 and IgG2 as well as IgA1 and IgA2 between patients in acute phase and controls (p>0.05). Patients in acute phase had significantly increased IgG3, IgG4 and proteins in comparison to the controls (p=0,021; p=0,004; p=0,029). No significant differences could be found between patients in acute phase and during remission period in IgG1,2,3,4 and IgA1 while IgA2 was significantly increased in acute phase in comparison to the remission period (p=0,049). Between patients in remission period and controls there were no significant differences in any IgA or IgG salivary subclasses (p>0,05). We can conclude that acute phase is characterized with increase in IgA2 which might reflect increased activity of secretory immunity as a possible result of microbial stimulation seen in acute phase in comparison to the remission period

Salivary interleukin-6 and tumor necrosis factor-α in patients with recurrent aphthous ulceration

Background: Recurrent apthous ulceration (RAU) is a well known oral disease which seems to be mediated principally by the immune system. However, it is still a matter of debate which part of the immune system is implicated in its pathogenesis as a reaction to the still unknown antigen. The aim of this study was to evaluate salivary cytokines, interleukin (IL)-6, and tumor necrosis factor (TNF)-α. Methods: In 26 patients with minor RAU, age range of 23-49 years (mean 27.3 years), during both the acute phase and remission and in 26 healthy controls, age range of 22-64 years (mean 30.1 years), salivary IL-6 and TNF-α levels were determined by use of enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed by use of descriptive statistics. Results: Significant differences in salivary TNF-α between healthy controls and patients with acute RAU and during the remission period were found (P < 0.001) as well as between patients with acute RAU and those during the remission period (P < 0.001). No differences in salivary IL-6 between all three groups could be found. Conclusions: We might conclude that elevated salivary TNF-α levels during acute RAU and especially during the remission period are of importance in RAU, whereas salivary IL-6 levels seem not to play a role in the RAU disease