722 research outputs found
Keukentafelgesprekken Waardewerken
In het praktijknetwerk Waardewerken werken multifunctionele ondernemers samen met onderzoekers van Wageningen UR aan de uitdaging om de multifunctionele landbouw verder te versterken en te ontwikkelen tot een professionele en krachtige sector. Tijdens de keukentafelgesprekken worden de zorgpunten en eventuele oplossingen over de multifunctionele landbouw aangegeven
Distribution of endocrine cells in the gut of the impala (Aepyceros melampus)
Immunocytochemical methods were employed to demonstrate endocrine cells, containing peptides and
serotonin, in the gut of the impala. Cells immunoreactive to serotonin, somatostatin, gastrin, cholecystokinin,
glucagon, neurotensin, secretin, glucose-dependant insulinotropic peptide and motilin were detected.
Antisera raised to substance P and pancreatic polypeptide failed to stain any cells. The distribution
of these peptide-containing cells is more in line with the situation in sheep than other ruminants. In
contrast, the distribution and abundance of serotonin cells in the gut of the impala parallels the situation
seen in game herbivores.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201
The oesophagus and stomach of the African elephant : a histological, immunocytochemical and immunofluorescence study
Histological, immunocytochemical and immunofluorescence methods were employed to study the oesophagus
and stomach of the elephant. The histological findings were in line with the situation in monogastric
species like swine and man. In the mucosa of the stomach, endocrine cells were immunoreactive to
gastrin, somatostatin, chromogranin A and serotonin. Nerve cells immunoreactive to somatostatin,
bombesin, VIP, PHI and CGRP were detected in the submucosal and myenteric plexus of the stomach.
In the stomach, the absence of glucagon cells and the presence of endocrine cells immunoreactive to
PYY, are in contrast to the situation in mammals and need further investigation. Small gastric ulcers
were observed in some of the specimens.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201
Evolution of coronary atherosclerosis in patients with mild coronary artery disease studied by serial quantitative coronary angiography at 2 and 4 years follow up
AIMS: Angiographic studies on the natural course of both focal and diffuse coronary atherosclerosis have not been performed before, but can both be assessed by quantitative coronary angiography. The objective of this study was to describe the natural course of focal and diffuse coronary atherosclerosis over time. METHODS AND RESULTS: In 129 patients with mild coronary artery disease, but not on lipid-lowering medication, three coronary angiograms were made each 2 years apart. Nine hundred and sixty five angiographically diseased and non-diseased segments were analysed by quantitative coronary angiography. Mean lumen diameter and minimal lumen diameter were used as measures of diffuse and focal coronary atherosclerosis. Mean lumen diameter and minimum lumen diameter decreased by 0.02 and 0.03 mm per year. The rate of progression was similar in the angiographically non-diseased, as in the mildly and moderately diseased segments. Progression of diffuse coronary atherosclerosis was largest in severely stenosed lesions (percentage diameter stenosis > or = 50%) and in the right coronary artery with a loss of 0.19 mm and 0.16 mm in mean lumen diameter. Progression of focal disease was most prominent in new and mild lesions and the right coronary artery, with a decrease in minimum lumen diameter of 0.34 mm and 0.22 mm. In most subgroups, progression occurred gradually over time. On a per segment level, progression and the occurrence of new lesions occurred in 4.4% and 4.2%. Regression and disappearance of a lesions was found in 2.3% and 1.9%. On a per patient level, 36% were progressors, 12% had a mixed response, 36% were stable, and 16% were regressors. CONCLUSION: Diffuse and focal coronary atherosclerosis progressed at the same rate in the first and second 2 years in stenosed and non-stenosed segments. The rate of coronary atherosclerosis progression was small, but was higher for focal than for diffuse disease. A minority of lesions progressed and spontaneous regression was rare
The intestine and endocrine pancreas of the African elephant: a histological, immunocytochemical and immunofluorescence study
Histological, immunocytochemical and immunofluorescence methods were employed to study the intestine
and endocrine pancreas of the elephant. The histological findings were in line with those in
monogastric mammals. In the mucosa of intestine, endocrine cells were immunoreactive to somatostatin,
gastrin, CCK, GIP, secretin, motilin, glucagon and NPY. Nerve cells immunoreactive to somatostatin,
substance P, VIP, PHI, NPY, bombesin and CGRP were detected. No immunoreactivity to neurotensin
was observed. Islets of the pancreas had insulin cells in their cores and glucagon and somatostatin
cells in their mantles. The antisera employed failed to demonstrate PP cells in the pancreas, but NPY immunoreactive
cells were present.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.PJ. de Villiers Booksellers,
Bloemfontein.mn201
Alteration of the Exhaled Volatile Organic Compound Pattern in Colorectal Cancer Patients after Intentional Curative Surgery—A Prospective Pilot Study
As current follow-up modalities for colorectal carcinoma (CRC) have restricted sensitivity, novel diagnostic tools are needed. The presence of CRC changes the endogenous metabolism, resulting in the release of a specific volatile organic compounds (VOC) pattern that can be detected with an electronic nose or AeonoseTM. To evaluate the use of an electronic nose in the follow-up of CRC, we studied the effect of curative surgery on the VOC pattern recognition using AeonoseTM. A prospective cohort study was performed, in which 47 patients diagnosed with CRC were included, all of whom underwent curative surgical resection. Breath testing was performed before and after surgery using the AeonoseTM. A machine learning model was developed by discerning between the 94 pre-and postoperative breath samples. The training model differentiated between the pre-and postoperative CRC breath samples with a sensitivity and specificity of 0.78 (95%CI 0.61–0.90) and 0.73 (95%CI 0.56–0.86), respectively, with an accuracy of 0.76 (95%CI 0.66–0.85), and an area under the curve of 0.79 (95%CI 0.68–0.89). The internal validation of the test set resulted in an accuracy of 0.75 (95%CI 0.51–0.91) and AUC of 0.82 (95%CI 0.61–1). In conclusion, our results suggest that the VOC pattern of CRC patients is altered by curative surgery in a short period, indicating that the exhaled VOCs might be closely related to the presence of CRC. However, to use AeonoseTM as a potential diagnostic tool in the clinical follow-up of CRC patients, the performance of the models needs to be improved through further large-scale prospective research.</p
Effectiveness of a high volume injection as treatment for chronic Achilles tendinopathy
Objective
To study whether a high volume injection without
corticosteroids improves clinical outcome in addition
to usual care for adults with chronic midportion
Achilles tendinopathy.
Design
Patient and assessor blinded, placebo controlled
randomised clinical trial.
Setting
Sports medicine department of a large district general
hospital, the Netherlands.
Participants
80 adults (aged 18-70 years) with clinically diagnosed
chronic midportion Achilles tendinopathy and
neovascula
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