420 research outputs found
Influence of spray drying suspension on the morphology of Fe-based oxygen carriers for chemical looping
Chemical looping reforming (CLR) and chemical looping combustion (CLC) are promising technologies with inherent CO2 capture for transforming fuels into syngas and energy respectively. Circulating oxygen carriers (OC) are used to transfer oxygen from mostly air to the fuel inside the process. Over the past years a variety of materials have been proposed for the role of oxygen carriers, ranging from bulk mineral powders to oxygen carrier particles engineered for shape, size and composition. Iron based materials are very promising and cost effective candidates with minor impact on the environment as compared to the toxic Ni-based OCs.
Granulation by the industrial spray-drying technique is suitable for producing oxygen carrier particles with high sphericity and dimensions fit for the fluidized-bed reactors of the CL-process. The lifetime of the oxygen carriers in these reactors however strongly depends on their mechanical properties (as measured by the crushing strength and the attrition resistance) which is related with their morphology and porosity. As this morphology depends on the spray drying suspension, the relation between the additives used in the iron-based suspension and the morphology of the spray-dried particles is investigated in this work [1]. The influence of the concentration of the binder, dispersing agent and solids in the spray-drying suspensions and the intensity of the milling procedure on the morphology and microstructure of the resulting particles is studied by Hg-porosimetry, tapped density, optical microscopy and SEM. A controlled sintering treatment is used during post-processing of these spray-dried particles in order to further improve their mechanical properties before investigating their performance as oxygen carriers in the chemical looping process
Multilevel analysis of nuclear dynamics in lamin perturbed fibroblasts
The nuclear lamina provides structural support to the nucleus and has a central role in defining nuclear organization. Defects in its filamentous constituents, the lamins, lead to a class of diseases collectively referred to as laminopathies. On the cellular level, lamin mutations affect the physical integrity of nuclei and nucleo-cytoskeletal interactions, resulting in increased susceptibility to mechanical stress and altered gene expression [1]. Most studies regarding the mechanical properties of the nucleus in laminopathic conditions are based on the induction of extracellular stress, such as strain or compression, and focus on nuclear integrity and/or nucleo-cytoskeletal interaction [2]. Far less is known about the role of nuclear organization and mobility under basal steady-state conditions.
In this study, we quantitatively compared nuclear organization, nuclear deformation and chromatin mobility of fibroblasts from a Hutchinson-Gilford progeria patient with cells from a lamin A/C-deficient patient and wild-type dermal fibroblasts. To this end, we created a toolbox in imageJ for automatically analyzing both nuclear as well as subnuclear dynamics in living cells. Simultaneously, we developed a workflow for comparing cellular morphology and subcellular protein distribution in a high content fashion.
We found that the absence of functional lamin A/C leads to increased nuclear plasticity on the hour and minute time scale but also to increased intranuclear mobility down to the seconds time scale. In contrast, progeria cells showed overall reduced nuclear dynamics. In addition, high content analysis revealed marked morphological and topological differences between different culture passages within a cell type and between different pathological variants of culture-age matched laminopathic cell types
Automated detection of wedge-shaped defects in polarimetric images of the retinal nerve fibre layer
Purpose: Automated glaucoma detection in images obtained by scanning laser polarimetry is currently insensitive to local abnormalities, impairing its performance. The purpose of this investigation was to tes
Development of stable oxygen carrier materials for chemical looping processes : a review
This review aims to give more understanding of the selection and development of oxygen carrier materials for chemical looping. Chemical looping, a rising star in chemical technologies, is capable of low CO2 emissions with applications in the production of energy and chemicals. A key issue in the further development of chemical looping processes and its introduction to the industry is the selection and further development of an appropriate oxygen carrier (OC) material. This solid oxygen carrier material supplies the stoichiometric oxygen needed for the various chemical processes. Its reactivity, cost, toxicity, thermal stability, attrition resistance, and chemical stability are critical selection criteria for developing suitable oxygen carrier materials. To develop oxygen carriers with optimal properties and long-term stability, one must consider the employed reactor configuration and the aim of the chemical looping process, as well as the thermodynamic properties of the active phases, their interaction with the used support material, long-term stability, internal ionic migration, and the advantages and limits of the employed synthesis methods. This review, therefore, aims to give more understanding into all aforementioned aspects to facilitate further research and development of chemical looping technology
Sustainable Attrition-resistant oxygen carriers for chemical looping reforming by spray drying
Chemical looping reforming (CLR) and chemical looping combustion (CLC) are promising technologies with inherent CO2 capture for transforming fuels into syngas and energy respectively. Circulating oxygen carriers (OC) are used to transfer oxygen from mostly air to the fuel inside the process. Over the past years a variety of materials have been proposed for the role of oxygen carriers, ranging from bulk mineral powders to oxygen carrier particles engineered for shape, size and composition. A lot of attention has been focused towards the development of Ni-based oxygen carriers due to their good reactivity, conversion and mechanical stability. However the cost, susceptibility towards S-containing impurities and their toxicity are key drivers to develop Ni-free materials. Oxygen carriers based on Fe-oxides are promising because of their lower cost and diminished impact on health and environment. Nevertheless, they also need good thermo-chemo-mechanical properties and thus a sufficient lifetime to be applicable in industrial CL-processes. The longevity of the oxygen carriers in the coupled fluidised bed reactors can be limited by two factors, such as their fragmentation and attrition leading to smaller particles inhibiting their fluidisation as well as their chemical deactivation.
Granulation by the industrial spray drying technique appears to yield oxygen carrier particles with high sphericity and good fluidization properties. In addition, a microstructure is obtained that on the one hand aims at thermo-chemo-mechanical integrity and high attrition resistance, and on the other hand at intimate contact between the solid and gaseous phases. However, spray-drying does not appear to be used for producing Fe-based OC thus far.
This work emphasises the colloid chemistry of concentrated suspensions used for spray-drying, the development of Fe-based OC by granulation of primary raw materials and the subsequent processing and heat treatment of the oxygen carriers for hydrogen production by chemical looping. To reach the objectives, a systematic approach is used. At first the role of the preparation of the spray dry suspension and the granulation conditions on the morphology of the particles has been investigated. Secondly, the effect of the sinter process was studied. A correlation between tapped density, strength and attrition resistance of the heat-treated materials was observed. Finally, the chemical performance of these Fe-based oxygen carriers was examined in a small scale batch reactor. The chemical composition of the OC was (ex situ) monitored in view of the enhancement of the chemical properties and long term stability thereof by altering the composition and microstructure of synthesised oxygen carriers
Automated Detection of Cribriform Growth Patterns in Prostate Histology Images
Cribriform growth patterns in prostate carcinoma are associated with poor
prognosis. We aimed to introduce a deep learning method to detect such patterns
automatically. To do so, convolutional neural network was trained to detect
cribriform growth patterns on 128 prostate needle biopsies. Ensemble learning
taking into account other tumor growth patterns during training was used to
cope with heterogeneous and limited tumor tissue occurrences. ROC and FROC
analyses were applied to assess network performance regarding detection of
biopsies harboring cribriform growth pattern. The ROC analysis yielded a mean
area under the curve up to 0.81. FROC analysis demonstrated a sensitivity of
0.9 for regions larger than 0.0150 mm2 with on average 7.5 false positives. To
benchmark method performance for intra-observer annotation variability, false
positive and negative detections were re-evaluated by the pathologists.
Pathologists considered 9% of the false positive regions as cribriform, and 11%
as possibly cribriform; 44% of the false negative regions were not annotated as
cribriform. As a final experiment, the network was also applied on a dataset of
60 biopsy regions annotated by 23 pathologists. With the cut-off reaching
highest sensitivity, all images annotated as cribriform by at least 7/23 of the
pathologists, were all detected as cribriform by the network and 9/60 of the
images were detected as cribriform whereas no pathologist labelled them as
such. In conclusion, the proposed deep learning method has high sensitivity for
detecting cribriform growth patterns at the expense of a limited number of
false positives. It can detect cribriform regions that are labelled as such by
at least a minority of pathologists. Therefore, it could assist clinical
decision making by suggesting suspicious regions.Comment: 15 pages, 6 figure
De kracht van draagkracht:Versterken zelforganiserend vermogen van inwoners: Eindrapportage onderzoek
Improved reliability of perfusion estimation in dynamic susceptibility contrast MRI by using the arterial input function from dynamic contrast enhanced MRI
The arterial input function (AIF) plays a crucial role in estimating quantitative perfusion properties from dynamic susceptibility contrast (DSC) MRI. An important issue, however, is that measuring the AIF in absolute contrast-agent concentrations is challenging, due to uncertainty in relation to the measured (Formula presented.) -weighted signal, signal depletion at high concentration, and partial-volume effects. A potential solution could be to derive the AIF from separately acquired dynamic contrast enhanced (DCE) MRI data. We aim to compare the AIF determined from DCE MRI with the AIF from DSC MRI, and estimated perfusion coefficients derived from DSC data using a DCE-driven AIF with perfusion coefficients determined using a DSC-based AIF. AIFs were manually selected in branches of the middle cerebral artery (MCA) in both DCE and DSC data in each patient. In addition, a semi-automatic AIF-selection algorithm was applied to the DSC data. The amplitude and full width at half-maximum of the AIFs were compared statistically using the Wilcoxon rank-sum test, applying a 0.05 significance level. Cerebral blood flow (CBF) was derived with different AIF approaches and compared further. The results showed that the AIFs extracted from DSC scans yielded highly variable peaks across arteries within the same patient. The semi-automatic DSC–AIF had significantly narrower width compared with the manual AIFs, and a significantly larger peak than the manual DSC–AIF. Additionally, the DCE-based AIF provided a more stable measurement of relative CBF and absolute CBF values estimated with DCE–AIFs that were compatible with previously reported values. In conclusion, DCE-based AIFs were reproduced significantly better across vessels, showed more realistic profiles, and delivered more stable and reasonable CBF measurements. The DCE–AIF can, therefore, be considered as an alternative AIF source for quantitative perfusion estimations in DSC MRI.</p
A novel combination of fipronil and permethrin (Frontline Tri-Act®/Frontect®) reduces risk of transmission of Babesia canis by Dermacentor reticulatus and of Ehrlichia canis by Rhipicephalus sanguineus ticks to dogs
BACKGROUND : The ability of Frontline Tri-Act®/Frontect®, a topical ectoparasiticide containing fipronil and permethrin
for dogs, to prevent the transmission of Babesia canis as well as Ehrlichia canis was evaluated by infesting dogs with
infected vector ticks.
METHODS : For the Babesia canis study, 16 dogs were randomly allocated to two groups. Eight dogs were treated on
day 0 with a topical spot-on formulation containing 6.76 % w/v fipronil plus 50.48 % w/v permethrin and eight dogs
served as the untreated control group. Dermacentor reticulatus ticks, with a B. canis infection rate ranging between
2 and 10 %, were placed onto dogs on days 7, 14, 21 and 28. In situ tick counts were performed on Days 9, 16 and
23. Ticks were counted and removed on Day 30. Infection of the dogs with B. canis was monitored by rectal temperature
readings, clinical examinations and blood smears as well as PCR and IFA (indirect fluorescent antibody assay).
For the Ehrlichia canis study, another 16 dogs were allocated to two groups. Eight dogs were treated with the fipronil and
permethrin combination on days 0 and 28 and eight dogs served as untreated controls. Rhipicephalus sanguineus ticks,
carrying an infection rate of 13 % for E. canis, were released in the sleeping kennels of the dogs on days 7, 14, 21, 28, 35,
42, 49 and 56. Ticks were counted in situ on the dogs on a weekly basis. All ticks were removed and counted on the final
assessment day 58. Infection of the dogs with E. canis was monitored by rectal temperature, clinical examinations, and
testing of blood samples by PCR, IFA and platelet counts.
RESULTS : B. canis was transmitted by D. reticulatus ticks to all eight untreated control dogs and to one treated dog, which
was confirmed by blood smears, PCR and IFA. E.canis was transmitted by R. sanguineus ticks to all eight untreated control
dogs. Two of the dogs in the treated group were found positive based on PCR and/or IFA.
CONCLUSIONS : Frontline Tri-Act®/Frontect® significantly lowered the risk for dogs to acquire a B. canis infection by 87.5 %
over a challenge period of 28 days. The risk for dogs to acquire E. canis was reduced by 75 % over a period of 56 days.Merial S.A.S., a Sanofi company, France.http://www.parasitesandvectors.comam201
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