Risk factors and prediction of physical problems in children induced by nervous system diseases

It was shown that in the conditions of intensive development of resuscitation techniques, newborn care and infant mortality reduction, including born with extremely low birth weight, one of the consequences of the process is the growing number of children with disabilities. In Russia, the disability of children under 4 years due to diseases of the nervous system consistently takes second place as on prevalence, as on the share in the structure of disability in this age. The purpose of the study was to identify risk factors and the development of prognostic tables for the most common early childhood disorders of physical health of children with disabilities due to diseases of the nervous system. A clinical examination of 178 children with disabilities due to diseases of the nervous system (with spastic forms of cerebral palsy) at the age of 1–3 years was carried out. To identify the risk factors and to draft the expectancy table, the method of sequential mathematical analysis of Wald has been used. It is shown that the priority kinds of physical health disorders of studied children with disabilities are frequent acute respiratory infections, deficient anemia, malnutrition, atopic dermatitis. It was found that during the formation of the frequent incidence of disabled children with acute respiratory diseases and atopic dermatitis neonatal and post-neonatal factors in the formation of deficiency anemia and malnutrition – antenatal and neonatal risk factors are of the greatest importance. Tables of health disorders suitable for practical use of these predictions were designed. Pediatricians recommended to include children with poor prognosis in the risk group for the formation of these health problems and prescribe preventive measures that reduce the likelihood of risk realization

THE QUALITY OF PROFESSIONAL TRAINING OF THE GRADUATES (BY THE ESTIMATION OF EMPLOYER)

In this paper the results of sociological research on a problem of quality of professional training of graduates from educational institution of the high, middle and initial professional training in region (on an example of Sverdlovsk area) are presented. The basic problems in the field of preparation of qualified personnel to fit the requirements of business system of professional training are displayed. The directions of forming regional model of cooperation in the sphere of personnel’s preparation and retraining of are shown

Risk factors and prediction chart of violations of health of the one-year-olds born with very low and extremely low birth weight

The study revealed biological and social risk factors for the formation of life of the one-year-olds such as the low length, deficit of body weight, delay of mental development, frequent acute respiratory infections, the formation of cerebral palsy in children born with very low and extremely low birth weight. In identifying risk factors and prognostic drafting tables the method of sequential mathematical analysis of Wald has been used. It was found that the greatest influence on the formation of these health disorders in children with birth weight less than 1500g have the biological risk factors – health status and age of the mother, during pregnancy and childbirth, the child's health condition in the neonatal period. An algorithm for predicting the data of health disorders in the child's admission with a birth weight less than 1500 grams under the supervision of the district pediatrician

Supplementary Material for: Genomic Organization and Physical Mapping of Tandemly Arranged Repetitive DNAs in Sterlet (Acipenser ruthenus)

Acipenseriformes represent a phylogenetically basal clade of ray-finned fish characterized by unusual genomic traits, including paleopolyploid states of extant genomes with high chromosome numbers and slow rates of molecular evolution. Despite a high interest in this fish group, only a limited number of studies have been accomplished on the isolation and characterization of repetitive DNA, karyotype standardization is not yet complete, and sex chromosomes are still to be identified. Here, we applied next-generation sequencing and cluster analysis to characterize major fractions of sterlet (<i>Acipenser ruthenus</i>) repetitive DNA. Using FISH, we mapped 16 tandemly arranged sequences on sterlet chromosomes and found them to be unevenly distributed in the genome with a tendency to cluster in particular regions. Some of the satellite DNAs might be used as specific markers to identify individual chromosomes and their paralogs, resulting in the unequivocal identification of at least 18 chromosome pairs. Our results provide an insight into the characteristic genomic distribution of the most common sterlet repetitive sequences. Biased accumulation of repetitive DNAs in particular chromosomes makes them especially interesting for further search for cryptic sex chromosomes. Future studies of these sequences in other acipenserid species will provide new perspectives regarding the evolution of repetitive DNA within the genomes of this fish order.<br

Analysis of NotI linking clones isolated from human chromosome 3 specific libraries

We have partially sequenced more than 1000 NotI linking clones isolated from human chromosome 3-specific libraries. Of these clones, 152 were unique chromosome 3-specific clones. The clones were precisely mapped using a combination of fluorescence in situ hybridization (FISH) and hybridization to somatic cell or radiation hybrids. Two- and three-color FISH was used to order the clones that mapped to the same chromosomal region, and in some cases, chromosome jumping was used to resolve ambiguous mapping. When this NotI restriction map was compared with the yeast artificial chromosome (YAC) based chromosome 3 map, significant differences in several chromosome 3 regions were observed. A search of the EMBL nucleotide database with these sequences revealed homologies (90–100%) to more than 100 different genes or expressed sequence tags (ESTs). Many of these homologies were used to map new genes to chromosome 3. These results suggest that sequencing NotI linking clones, and sequencing CpG islands in general, may complement the EST project and aid in the discovery of all human genes by sequencing random cDNAs. This method may also yield information that cannot be obtained by the EST project alone; namely, the identification of the 5′ ends of genes, including potential promoter/enhancer regions and other regulatory sequence