Spatially-explicit model of sole larvae in the Southern North Sea: sensitivity of the dispersal to hydrodynamic/environment variability and biological parameters

Sole (Solea solea) is one of the most valuable commercial species in the North Sea. The size of the spawning stock is above the level of sustainable exploitation, but fishing mortality is high. The stock is therefore at risk of being harvested unsustainably. Moreover, interannual recruitment variability is very high. It is crucial to understand the contribution of hydrodynamics, environment and biological parameters to recruitment variability in order to propose appropriate measures for the management of the North Sea stock. Here we use a particle-tracking transport model coupled to a 3D hydrodynamic model to study the relative effect of hydrodynamic variability, environment variability (throughout temperature) and biological parameters on the dispersal of sole larvae in the Southern North Sea

Coupled analysis of a backfill hydration test

BACCHUS2 in situ isothermal wetting experiment has been analysed by means of a coupled flow-deformation approach. Backfill material, a mixture of Boom clay powder and high density pellets, has been extensively tested in the laboratory in order to determine its hydraulic and mechanical properties. Parameters of constitutive equations were derived from this experimental data base. Two mechanical constitutive models have been used in the simulation of the 'in situ' experiment: a state surface approach and an elastoplastic model. Calculations have shown several features of the hydration process which help to understand the behaviour of expansive clay barriers. Predictions using both models have been compared with each other and with actual measurement records. This has allowed a discussion of the comparative mertis of both approaches and the identiÞcation of some critical parameters of backfill behaviour. Overall agreement between calculations and field measurements is encouraging and shows the potential of the methods developed to model the behaviour of engineered clay barriers in the context of nuclear waste disposal

Suction-controlled experiments on Boom clay

The understanding of the thermo-hydro-mechanical behaviour of a clay barrier is needed for the prediction of its final in situ properties after the hydration and thermal transient in a radioactive waste repository. As part of the CEC 1990-1994 R&D programme on radioactive waste management and storage, the CEA (Fr), CIEMAT (Sp). ENRESA (Sp). SCK CEN (B). UPC (Sp) and UWCC (UK) have carried out a joint project on unsaturated clay behaviour (Volckaert et al.. 1996). The aim of the study is to analyse and model the behaviour of a clay-based engineered barrier during its hydration phase under real repository conditions. The hydro-mechanical and thermo-hydraulic models developed in this project have been coupled to describe stress/strain behaviour, moisture migration and heat transfer. A thermo-hydraulic model has also been coupled to a geochemical code to describe the migration and formation of chemical species. In this project. suction-controlled experiments have been performed on Boom clay (B), FoCa clay (Fr) and Almeria bentonite (Sp). The aim of these experiments is to test the validity of the interpretive model developed by Alonso and Gens (Alonso et al., 1990). and to build a database of unsaturated clay thermo-hydro-mechanical parameters. Such a database can then be used for validation exercises in which in situ experiments are simulated. The Boom clay is a moderately swelling clay of Rupellian age. It is studied at the SCK. CEN in Belgium as a potential host rock for a radioactive waste repository. In this paper, suction-controlled experiments carried out on Boom clay by SCK CEN are described. SCK CEN has performed experiments to measure the relation between suction, water content and temperature and the relation between suction, stress and deformation. The applied suctioncontrol techniques and experimental setups are detailed. The results of these experiments are discussed in the perspective of the model of Alonso and Gens. The influence of temperature on water uptake was rather small. The measured swelling-collapse behaviour can be explained by the Alonso and Gens model.Peer Reviewe

The European sea bass <i>Dicentrarchus labrax</i> genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing

BackgroundFood supply from the ocean is constrained by the shortage of domesticated and selected fish. Development of genomic models of economically important fishes should assist with the removal of this bottleneck. European sea bass Dicentrarchus labrax L. (Moronidae, Perciformes, Teleostei) is one of the most important fishes in European marine aquaculture; growing genomic resources put it on its way to serve as an economic model.ResultsEnd sequencing of a sea bass genomic BAC-library enabled the comparative mapping of the sea bass genome using the three-spined stickleback Gasterosteus aculeatus genome as a reference. BAC-end sequences (102,690) were aligned to the stickleback genome. The number of mappable BACs was improved using a two-fold coverage WGS dataset of sea bass resulting in a comparative BAC-map covering 87% of stickleback chromosomes with 588 BAC-contigs. The minimum size of 83 contigs covering 50% of the reference was 1.2 Mbp; the largest BAC-contig comprised 8.86 Mbp. More than 22,000 BAC-clones aligned with both ends to the reference genome. Intra-chromosomal rearrangements between sea bass and stickleback were identified. Size distributions of mapped BACs were used to calculate that the genome of sea bass may be only 1.3 fold larger than the 460 Mbp stickleback genome.ConclusionsThe BAC map is used for sequencing single BACs or BAC-pools covering defined genomic entities by second generation sequencing technologies. Together with the WGS dataset it initiates a sea bass genome sequencing project. This will allow the quantification of polymorphisms through resequencing, which is important for selecting highly performing domesticated fish

Coupled analysis of a backfill hydration test

BACCHUS2 in situ isothermal wetting experiment has been analysed by means of a coupled flow-deformation approach. Backfill material, a mixture of Boom clay powder and high density pellets, has been extensively tested in the laboratory in order to determine its hydraulic and mechanical properties. Parameters of constitutive equations were derived from this experimental data base. Two mechanical constitutive models have been used in the simulation of the 'in situ' experiment: a state surface approach and an elastoplastic model. Calculations have shown several features of the hydration process which help to understand the behaviour of expansive clay barriers. Predictions using both models have been compared with each other and with actual measurement records. This has allowed a discussion of the comparative mertis of both approaches and the identiÞcation of some critical parameters of backfill behaviour. Overall agreement between calculations and field measurements is encouraging and shows the potential of the methods developed to model the behaviour of engineered clay barriers in the context of nuclear waste disposal.Peer Reviewe

Efficiency of a borehole seal by means of pre-compacted bentonite blocks

The backfilling and sealing of shafts and galleries is an essential part of the design of underground repositories for high-level radioactive waste. Part of the EC funded project RESEAL studied the feasibility of sealing off a borehole in plastic Boom Clay by means of pre-compacted bentonite blocks. Two bentonites, namely the FoCa and Serrata clay, have been used. Based on laboratory tests, the bentonite blocks had an initial dry density of about 1.8 g/cm3 to obtain a swelling pressure of about 4.4 MPa, corresponding to the in situ lithostatic stress, at full saturation. The set-up was equipped with several sensors to follow-up the behaviour of the seal and the surrounding host rock during hydration. Full saturation was reached after five months and was mainly reached by natural hydration. Swelling pressure was lower than originally foreseen due to the slow reconsolidation of the host rock. Later on, the efficiency of the seal with respect to water, gas and radionuclide migration was tested. The in situ measured permeability of the seals was about 5 &times; 10-13 m/s. A gas breakthrough experiment did not show any preferential gas migration through the seal. No evidences of a preferential pathway could be detected from 125I tracer test result