8 research outputs found
Unveiling the excited state energy transfer pathways in peridinin-chlorophyll Ī±-protein by ultrafast multi-pulse transient absorption spectroscopy
Time-resolved multi-pulse methods were applied to investigate the excited state dynamics, the interstate couplings, and the excited state energy transfer pathways between the light-harvesting pigments in peridinin-chlorophyll a-protein (PCP). The utilized pump-dump-probe techniques are based on perturbation of the regular PCP energy transfer pathway. The PCP complexes were initially excited with an ultrashort pulse, resonant to the SāĀ āĀ Sā transition of the carotenoid peridinin. A portion of the peridinin-based emissive intramolecular charge transfer (ICT) state was then depopulated by applying an ultrashort NIR pulse that perturbed the interaction between Sā and ICT states and the energy flow from the carotenoids to the chlorophylls. The presented data indicate that the peridinin Sā and ICT states are spectrally distinct and coexist in an excited state equilibrium in the PCP complex. Moreover, numeric analysis of the experimental data asserts ICTĀ āĀ Chl-Ī± as the main energy transfer pathway in the photoexcited PCP systems.11 page(s
Synthesis, crystal structures, and laser flash photolysis of 3-nitro-7a,15-methanonaphtho[1',2':6,7][1,3]oxazepino[3,2-a]indole derivatives
The condensation of 1-substituted 9,9a-dihydro-1H-imidazo[1,2-a]indol-2(3H)-ones with 2- hydroxy-6-nitro-1-naphthaldehyde afforded 1ā²-carbamoylmethyl-8-nitrospiro[benzo[f]chromene- 3,2ā²-indole] derivatives, which underwent intramolecular cyclisation to derivatives of 3-nitro- 7a,15-methanonaphtho[1ā²,2ā²:6,7][1,3]oxazepino[3,2-a]indole upon treatment with a strong base. Laser excitation of the obtained uncoloured molecules of trans- and cis-3-nitro-7a,15-methanonaphtho[ 1ā²,2ā²:6,7][1,3]oxazepino[3,2-a]indole induced the formation of short-lived photogenerated species, which absorb in the visible spectrum and thermally revert to the ground state on a nanosecond time scale
Optically Controlled Molecular Switching of an Indolobenzoxazine-Type Photochromic Compound
Photochromic
forward (oxazine ring-opening) and backward (oxazine
ring-closing) switching dynamics of an indolobenzoxazine compound
were studied by femtosecond pumpārepumpāprobe technique.
A UV pulse was used to excite the ring-closed form of the photochromic
compound, causing a CāO bond cleavage and the formation of
a spectrally red-shifted isomer within a time scale of ca. 100 ps.
A successive, temporally delayed near-IR pulse, resonant to the red-most
absorption maximum of the ring-opened form, was used to reexcite the
molecular system, causing a fast photoinduced oxazine ring closure,
thereby āshort-circuitingā the normally nanosecond lasting
photocycle and returning ā¼6% of the molecules to the main molecular
ground state. Two possible models, involving the <i>S</i><sub>1</sub> excited state of the terminal photoproduct and its hot
ground state, are introduced to explain the pre- and post-reexcitation
spectral development and the photoinduced switching back mechanics
Structure and properties of tethered bilayer lipid membranes with unsaturated anchor molecules.
The self-assembled monolayers (SAMs) of new lipidic anchor molecule HC18 [Z-20-(Z-octadec-9-enyloxy)-3,6,9,12,15,18,22-heptaoxatetracont-31-ene-1-thiol] and mixed HC18/Ī²-mercaptoethanol (Ī²ME) SAMs were studied by spectroscopic ellipsometry, contact angle measurements, reflection-absorption infrared spectroscopy, and electrochemical impedance spectroscopy (EIS) and were evaluated in tethered bilayer lipid membranes (tBLMs). Our data indicate that HC18, containing a double bond in the alkyl segments, forms highly disordered SAMs up to anchor/Ī²ME molar fraction ratios of 80/20 and result in tBLMs that exhibit higher lipid diffusion coefficients relative to those of previous anchor compounds with saturated alkyl chains, as determined by fluorescence correlation spectroscopy. EIS data shows the HC18 tBLMs, completed by rapid solvent exchange or vesicle fusion, form more easily than with saturated lipidic anchors, exhibit excellent electrical insulating properties indicating low defect densities, and readily incorporate the pore-forming toxin Ī±-hemolysin. Neutron reflectivity measurements on HC18 tBLMs confirm the formation of complete tBLMs, even at low tether compositions and high ionic lipid compositions. Our data indicate that HC18 results in tBLMs with improved physical properties for the incorporation of integral membrane proteins (IMPs) and that 80% HC18 tBLMs appear to be optimal for practical applications such as biosensors where high electrical insulation and IMP/peptide reconstitution are imperative.</p
Structure and Properties of Tethered Bilayer Lipid Membranes with Unsaturated Anchor Molecules
The
self-assembled monolayers (SAMs) of new lipidic anchor molecule
HC18 [<i>Z</i>-20-(<i>Z</i>-octadec-9-enyloxy)-3,6,9,12,15,18,22-heptaoxatetracont-31-ene-1-thiol]
and mixed HC18/Ī²-mercaptoethanol (Ī²ME) SAMs were studied
by spectroscopic ellipsometry, contact angle measurements, reflectionāabsorption
infrared spectroscopy, and electrochemical impedance spectroscopy
(EIS) and were evaluated in tethered bilayer lipid membranes (tBLMs).
Our data indicate that HC18, containing a double bond in the alkyl
segments, forms highly disordered SAMs up to anchor/Ī²ME molar
fraction ratios of 80/20 and result in tBLMs that exhibit higher lipid
diffusion coefficients relative to those of previous anchor compounds
with saturated alkyl chains, as determined by fluorescence correlation
spectroscopy. EIS data shows the HC18 tBLMs, completed by rapid solvent
exchange or vesicle fusion, form more easily than with saturated lipidic
anchors, exhibit excellent electrical insulating properties indicating
low defect densities, and readily incorporate the pore-forming toxin
Ī±-hemolysin. Neutron reflectivity measurements on HC18 tBLMs
confirm the formation of complete tBLMs, even at low tether compositions
and high ionic lipid compositions. Our data indicate that HC18 results
in tBLMs with improved physical properties for the incorporation of
integral membrane proteins (IMPs) and that 80% HC18 tBLMs appear to
be optimal for practical applications such as biosensors where high
electrical insulation and IMP/peptide reconstitution are imperative