2 research outputs found
Nonlinear Correlation Spectroscopy (NLCS)
We present a novel concept for optical spectroscopy called
nonlinear
correlation spectroscopy (NLCS). NLCS analyses coherent field fluctuations
of the second and third harmonic light generated by diffusing nanoparticles.
Particles based on noncentrosymmetric nonlinear materials such as
KNbO<sub>3</sub> show a strong second as well as third harmonic response.
The method and the theory are introduced and experimental NLCS results
in fetal calf serum are presented showing the promising selectivity
of this technique for measurement in complex biological environments
DataSheet_1_Highly Invasive Fluorescent/Bioluminescent Patient-Derived Orthotopic Model of Glioblastoma in Mice.docx
Development of the novel diagnostic and therapeutic approaches in neuro-oncology requires tumor models that closely reproduce the biological features of patientsâ tumors. Patient-derived xenografts (PDXs) are recognized as a valuable and the most âclose-to-patientâ tool for preclinical studies. However, their establishment is complicated by the factors related to both the surgical material and technique of the orthotopic implantation. The aim of this work was to develop a patient-derived glioblastoma multiform (GBM) model that stably co-expresses luciferase and a far-red fluorescent protein for monitoring of tumor progression in the brain and, using this model, to validate new diagnostic methodsâmacroscopic fluorescence lifetime imaging (macro-FLIM) and cross-polarization optical coherence tomography (CP OCT). The established model was similar to the original patientâs GBM in terms of histological and immunohistochemical features and possessed reproducible growth in nude mice, which could be observed by both fluorescence and bioluminescence imaging. Our results demonstrated the high potential of macro-FLIM and CP OCT for intraoperative differentiation of GBM from the white matter. Thus, the dual-labeled PDX model of GBM proved to be an excellent approach for observation of tumor development by optical methods.</p