7 research outputs found
Childrenâs migration and chronic illness among older parents âleft behindâ in China
The relationship between adult childrenâs migration and the health of their older parents âleft behindâ is an emerging research area and existing studies reflect mixed findings. This study aims to investigate the association between having migrant (adult) children and older parentsâ chronic illness in China, using chronic stomach or other digestive diseases as a proxy. Secondary analysis of the national baseline survey of the 2011 China Health and Retirement Longitudinal Study (CHARLS) was conducted. Analyses were conducted in a total of sample of 6495 individuals aged 60 years and above from 28 out of 31 provinces in China, who had at least one child at the baseline survey. Binary logistic regression was used. The prevalence of any of the diagnosed conditions of chronic stomach or other digestive diseases was higher among older people with a migrant son than among those without (27 percent vs 21 percent, p < 0.001). More specifically, the odds ratio of reporting a disease was higher among older adults with at least one adult son living in another county or province than among those with all their sons living closer (OR = 1.29, 95% CI = 1.10â1.51). The results from this large sample of older adults support the hypothesis that migration of sons significantly increases the risk of chronic stomach and other digestive diseases among âleft behindâ elderly parents in contemporary China
The structure of IS<i>256</i> and its extrachromosomal circular DNA in OC8.
<p>In A, the structure of IS<i>256</i> (OC8) is based on the OC8 genome sequence (GenBank accession number AP017377); the structure was very similar to previously described IS<i>256</i> structures [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164168#pone.0164168.ref027" target="_blank">27</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164168#pone.0164168.ref048" target="_blank">48</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164168#pone.0164168.ref049" target="_blank">49</a>]. PCR primers to detect an IS<i>256</i> circular DNA were designed based on the OC8 genome sequence. In B, the PCR primer set (R-R1 and L-R2, shown in A) exactly detected IS<i>256</i> circular DNA for OC8 (PCR product size, approximately 200 bp), while there were no amplified bands for strain USA300-0114, which lacked IS<i>256</i>. In C (and B), the 194-bp nucleotide sequence of the estimated PCR product, perfectly matched the IR<sub>L</sub> side and IR<sub>R</sub> side regions of IS<i>256</i> (OC8), and contained a 6-bp stretch, marked in red; 26-bp imperfect IR sequences and 6-bp stretch sequences were underlined in C. However, the 6-bp stretch data showed a âmixedâ result, with TTTTTT as the highest base content (followed by AAAAAA). Since the 6-bp stretch originates from a flanking <i>att</i> sequence [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164168#pone.0164168.ref048" target="_blank">48</a>] and OC8 carries 19 IS<i>256</i> copies with distinct <i>att</i> sequences, the âmixedâ 6-bp stretch reflects the presence of heterogeneous circular DNA (in terms of stretch sequences) in OC8. This observation is consistent with the AT-rich <i>att</i> sequences of 19 IS<i>256</i> copies on the genome.</p
Relevant characteristics of MRSA strains<sup>a</sup>.
<p>Relevant characteristics of MRSA strains<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164168#t001fn001" target="_blank"><sup>a</sup></a>.</p
PCR targeting the OC8-type megabase inversion (MbIN).
<p>In A and B, PCR primers targeting the junction sites of OC8 MbIN (A-C and B-D) and those targeting the corresponding region of USA300 FPR3757 (A-B and C-D) were designed based on the OC8 or USA300 FPR3757 complete genome sequence, respectively. The structures of the MbIN junction regions of OC8 and the corresponding regions of USA300 FPR3757 are from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164168#pone.0164168.g005" target="_blank">Fig 5</a>. In C to E, PCR products with an asterisk were sequenced, and the sequences determined were consistent with the OC8 or USA300 FPR3757 genome sequence. ST8<sub>Kras</sub> is ST8/SCC<i>mec</i>IVc MRSA from Krasnoyarsk, Siberian Russia. The geographical location of MRSA isolated in European Russia: Mow, Moscow; St. P, St. Petersburg; Yar, Yaroslavl. Regarding Far Eastern Russia, MRSA was isolated in Vladivostok.</p
Sequence comparison between OC8 and USA300 FPR3757 genomes and visualization of a large genomic inversion.
<p>Genomic sequence comparisons were performed using WebACT for the visualization of genomic inversions. The genome sequence of USA300 FPR3757 was from GenBank Accession number CP000255. The OC8 inverted region relative to USA300 FPR3757 is highlighted in blue.</p
Possible mechanisms for the large genomic inversion in OC8.
<p>In this model, shown in A, we hypothesized ancestor strains of OC8 for a one-megabase inversion (MbIN) and simultaneously-occurring deletion events. An initial ancestor strain (OC8 ancestor 1) lacks IS<i>256</i>, but has <i>att</i> site sequences, similar to USA300 FPR3757 (GenBank accession number CP000255); the size of OC8 ancestor 1 DNA flanked by two <i>att</i> sites on the right side of the figure was estimated to be 3,356 bp. The first step (step 1) includes three IS<i>256</i> insertions at different <i>att</i> sites. As shown on the right side of the figure, a homogenous recombination (step 2) then occurs between the direct repeats of IS<i>256</i> (in OC8 ancestor 1a), deleting a small region and leaving only one copy of IS<i>256</i> (generating OC8 ancestor 2). In step 3, a homogenous recombination subsequently occurs between the inverted repeats of IS<i>256</i> (on OC8 ancestor 2), with the one-megabase region being inverted, and generating OC8. The genes of NTPase, <i>hsdS</i>, and <i>hsdM</i> (on the top right side) were located in the genomic island vSAβ (marked with a red line). In B, figures focus on a vSAβ split event, which occurred simultaneously with MbIN. OC8 ancestor 1, OC8 ancestor 2, and OC8 are the same as those described in A. In C, a hypothetical folded chromosome structure with loop domains is illustrated, based on [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0164168#pone.0164168.ref057" target="_blank">57</a>], to boost the crossover and subsequent MbIN events at the two genomic locations, which are far from each other. (The diagram is not to scale.)</p
Table_1_Molecular Typing of ST239-MRSA-III From Diverse Geographic Locations and the Evolution of the SCCmec III Element During Its Intercontinental Spread.PDF
<p>ST239-MRSA-III is probably the oldest truly pandemic MRSA strain, circulating in many countries since the 1970s. It is still frequently isolated in some parts of the world although it has been replaced by other MRSA strains in, e.g., most of Europe. Previous genotyping work (Harris et al., 2010; Castillo-RamĂrez et al., 2012) suggested a split in geographically defined clades. In the present study, a collection of 184 ST239-MRSA-III isolates, mainly from countries not covered by the previous studies were characterized using two DNA microarrays (i) targeting an extensive range of typing markers, virulence and resistance genes and (ii) a SCCmec subtyping array. Thirty additional isolates underwent whole-genome sequencing (WGS) and, together with published WGS data for 215 ST239-MRSA-III isolates, were analyzed using in-silico analysis for comparison with the microarray data and with special regard to variation within SCCmec elements. This permitted the assignment of isolates and sequences to 39 different SCCmec III subtypes, and to three major and several minor clades. One clade, characterized by the integration of a transposon into nsaB and by the loss of fnbB and splE was detected among isolates from Turkey, Romania and other Eastern European countries, Russia, Pakistan, and (mainly Northern) China. Another clade, harboring sasX/sesI is widespread in South-East Asia including China/Hong Kong, and surprisingly also in Trinidad & Tobago. A third, related, but sasX/sesI-negative clade occurs not only in Latin America but also in Russia and in the Middle East from where it apparently originated and from where it also was transferred to Ireland. Minor clades exist or existed in Western Europe and Greece, in Portugal, in Australia and New Zealand as well as in the Middle East. Isolates from countries where this strain is not epidemic (such as Germany) frequently are associated with foreign travel and/or hospitalization abroad. The wide dissemination of this strain and the fact that it was able to cause a hospital-borne pandemic that lasted nearly 50 years emphasizes the need for stringent infection prevention and control and admission screening.</p