Localized expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development-1

<p><b>Copyright information:</b></p><p>Taken from "Localized expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development"</p><p>http://www.biomedcentral.com/1471-213X/7/117</p><p>BMC Developmental Biology 2007;7():117-117.</p><p>Published online 19 Oct 2007</p><p>PMCID:PMC2198918.</p><p></p>er while a black arrow indicates the pericardium region. (E-H) expression in , , and embryos at 48 hpf as indicated. (I – L) expression in various heterozygous and homozygous mutant embryos as indicated. Red arrows indicate the liver. Note precocious appearance of expression in homozygous mutant liver at 48 hpf (J, L) compared to heterozygous embryos (I, K). (M, N) expression in 48 hpf embryos treated with 10M RA initiated from 12 hpf (M) or 18 hpf (N). (O, P) expression in 48 hpf embryos treated with 10M DEAB initiated from 12 hpf (O) and 18 hpf (P)

Localized expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development-5

<p><b>Copyright information:</b></p><p>Taken from "Localized expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development"</p><p>http://www.biomedcentral.com/1471-213X/7/117</p><p>BMC Developmental Biology 2007;7():117-117.</p><p>Published online 19 Oct 2007</p><p>PMCID:PMC2198918.</p><p></p> marker. (B, C) Ventral (B) and lateral (C) view of 12 hpf embryos with expression as detected by WISH. (D) Ventral view of 16 hpf embryos with expression of (red) and (blue) as detected by two-colour WISH. (E) Cross section of the two color hybridized embyos in (D) as indicated by the dashed line. (F, F') Magnified view of boxed region F in Panel (E). F, bright field. F', compound image of DIC/fluorescence reveals expression and position of nuclei detected by DAPI staining. Arrows indicate YSL nuclei. (G), Magnified view of boxed region G in Panel (E)

Localized expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development-3

<p><b>Copyright information:</b></p><p>Taken from "Localized expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development"</p><p>http://www.biomedcentral.com/1471-213X/7/117</p><p>BMC Developmental Biology 2007;7():117-117.</p><p>Published online 19 Oct 2007</p><p>PMCID:PMC2198918.</p><p></p>dosage of Spl MO as indicated (B-D). The midline is indicated by a horizontal point/dash line. (E, F) Cross sections of the control embryo in (A) and morphant in (C) respectively. The section plane is indicated in (A, C) by the vertical dash line. Dashed circles in (E, F) represent gut and the vertical poin/dash lines indicate the midline. (G, H) Lateral view of expression in 24 hpf control embryo (G) and morphant (H). (I, J) Dorsal view of GFP expression in the principle islet of pancreas in 48 hpf control embryo (I) and morphant (J). (K-N) Control and morphant stained using two-color WISH with fluorescein-labeled (red) and Dig-labeled (blue). Panels (K, M) shows dorsal views of 3 dpf control and morphant respectively. Panels (L, N) are cross section at the planes as indicated in (K, M). Abbreviations:

Localized expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development-0

<p><b>Copyright information:</b></p><p>Taken from "Localized expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development"</p><p>http://www.biomedcentral.com/1471-213X/7/117</p><p>BMC Developmental Biology 2007;7():117-117.</p><p>Published online 19 Oct 2007</p><p>PMCID:PMC2198918.</p><p></p> marker. (B, C) Ventral (B) and lateral (C) view of 12 hpf embryos with expression as detected by WISH. (D) Ventral view of 16 hpf embryos with expression of (red) and (blue) as detected by two-colour WISH. (E) Cross section of the two color hybridized embyos in (D) as indicated by the dashed line. (F, F') Magnified view of boxed region F in Panel (E). F, bright field. F', compound image of DIC/fluorescence reveals expression and position of nuclei detected by DAPI staining. Arrows indicate YSL nuclei. (G), Magnified view of boxed region G in Panel (E)

Localized expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development-2

<p><b>Copyright information:</b></p><p>Taken from "Localized expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development"</p><p>http://www.biomedcentral.com/1471-213X/7/117</p><p>BMC Developmental Biology 2007;7():117-117.</p><p>Published online 19 Oct 2007</p><p>PMCID:PMC2198918.</p><p></p>ographed at 24 hpf and 48 hpf. (A, D) Control embryos at 24 hpf and 48 hpf. (B, E) Fluorescent lateral view image of type I phenotype (without yolk cell extension) morphants at 24 hpf (B) and 48 hpf (E). (C, F) fluorescent lateral view image of type II phenotype (short yolk cell extension) morphants at 24 hpf (C) and 48 hpf (F). White double arrows indicate the length and width of yolk extension. (G) Scheme of splicing morpholino and RT-PCR primer positions in the gene. Exons are represented by blue boxes with numbers and introns by white boxes. A red bar indicates the region targeted by Rbp4 Spl-MO. (H) RT-PCR analysis of RNAs from control (Ctrl) (non-injection)/splcing morphlino injected embryos (Spl-MO) and control (Ctrl) (non-injection)/mismatch morpholino injected embryos (Mis-MO) at 24 hpf using the primers as indicated in Panel (G) or control primers

Localized expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development-6

<p><b>Copyright information:</b></p><p>Taken from "Localized expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development"</p><p>http://www.biomedcentral.com/1471-213X/7/117</p><p>BMC Developmental Biology 2007;7():117-117.</p><p>Published online 19 Oct 2007</p><p>PMCID:PMC2198918.</p><p></p>er while a black arrow indicates the pericardium region. (E-H) expression in , , and embryos at 48 hpf as indicated. (I – L) expression in various heterozygous and homozygous mutant embryos as indicated. Red arrows indicate the liver. Note precocious appearance of expression in homozygous mutant liver at 48 hpf (J, L) compared to heterozygous embryos (I, K). (M, N) expression in 48 hpf embryos treated with 10M RA initiated from 12 hpf (M) or 18 hpf (N). (O, P) expression in 48 hpf embryos treated with 10M DEAB initiated from 12 hpf (O) and 18 hpf (P)

The roof plate formation in the <i>mib</i> mutant.

<p>(A), Confocal images, lateral view of the spinal cord of <i>mib</i> mutant, 72 hpf. Dashed rectangular shows the magnified in (A′) region of the spinal cord with the absence of GFP-positive RP cells. (B, C), the orthogonal optical sections of confocal images of the spinal cord of <i>mib</i> mutant illustrate lack of the roof plate extension between 36 and 72 hpf. Immunofluorescent staining of the transverse sections of spinal cord using anti-GFP (green) and anti-GFAP (red) antibodies; wild-type embryo (D–F) and <i>mib</i> mutant (G–I).</p

Characterization of SqET33 transgenic line.

<p>(A), Confocal image of 3 dpf larva of SqET33 line, lateral view. Dashed lines depict the position of transverse sections shown in B–D. (B–D), transverse sections, immunofluorescent staining with anti-GFP antibodies. Arrow indicates the elongated central process of the roof plate cell. (E–J), whole-mount immunofluorescent staining with anti-GFP (midline RP cells and lateral dorsal interneurons) and anti-HuC/HuD (lateral dorsal neurons) antibodies, dorsal view of spinal cord. (K–M), immunofluorescent staining with anti-GFP (green) and anti-GFAP (red) antibodies, transverse section of the spinal cord. Abbreviations: cc, central canal; dt, dorsal thalamus; ha, habenula; m, meninx; nc, notochord; p, pallium; po, preoptic area; rp, roof plate.</p

Re-orientation of the RP cells.

<p>Confocal images of the spinal cord of SqET33 line at different developmental stages (A–C, dorsal view, J–L, R, lateral view, M–P, orthogonal optical sections) and outline of the dorsal cells (D–F) superimposed onto the GFP intensity chart. Note that RB cells undergo the lateral-medial displacement. (G–I), Whole-mount immunohistochemistry detecting Islet1 in RB cells (red, nuclei), dorsal view of the spinal cord. (Q), Transverse section of the spinal cord at high magnification showing a fine structure of the central canal. RP process is stained with anti-GFP (green) and nuclei are counterstained with DAPI (grey). (S), Confocal image of the spinal cord of SqET33-10 line expressing GFP in the roof and floor plates, lateral view. Abbreviations: cc, central canal; di, dorsal interneurons; fp, floor plate; rb, Rohon-Beard cells; rp, roof plate cells.</p

Effect of Rock inhibition on the RP morphogenesis.

<p>Confocal images of 72 hpf larva, untreated (A) and after Y-27632 injection into hindbrain ventricle at 30 hpf (B). The arrows show an approximate position of the transverse sections. (C–E′), transverse sections of the spinal cord of larva, treated with Y-27632. Immunofluorescent staining with anti-GFP (green) and phalloidin (red). Whole mount <i>in situ</i> hybridization with <i>glyt1</i> antisense RNA probe, control (F) and after treatment with Y-27632 (G).</p