Identification of electrofacies on the basis of well logging to determine sedimentation environment of horizon JK[2] in Em-Egovskoe field (Western Siberia)

Well logging results are one of the ways to study the buried terrigenous rocks genesis. To ensure the most objective characterization of the rock and identification of electrofacies it is necessary to use a complex geological and geophysical survey. The comprehensive investigations of environmental conditions based on well logging have been performed for the horizon JK[2] of Tumenskoe formation in Em-Egovskoe area, Krasnoleninskoe field (Western Siberia). The defined electrofacies were compared with the results of earlier conducted granulometric and mineralogical analyses. The totality of research provided for a conclusion that the investigated sediments of horizon JK2 had been formed within the destructive tidal delta. Thus, objective facies prediction can only be ensured by analyzing core and well logging data comprehensively

Analysis of chromatin boundary activity in Drosophila cells

<p>Abstract</p> <p>Background</p> <p>Chromatin boundaries, also known as insulators, regulate gene activity by organizing active and repressive chromatin domains and modulate enhancer-promoter interactions. However, the mechanisms of boundary action are poorly understood, in part due to our limited knowledge about insulator proteins, and a shortage of standard assays by which diverse boundaries could be compared.</p> <p>Results</p> <p>We report here the development of an enhancer-blocking assay for studying insulator activity in Drosophila cultured cells. We show that the activities of diverse Drosophila insulators including suHw, SF1, SF1b, Fab7 and Fab8 are supported in these cells. We further show that double stranded RNA (dsRNA)-mediated knockdown of SuHw and dCTCF factors disrupts the enhancer-blocking function of suHw and Fab8, respectively, thereby establishing the effectiveness of using RNA interference in our cell-based assay for probing insulator function.</p> <p>Conclusion</p> <p>The novel boundary assay provides a quantitative and efficient method for analyzing insulator mechanism and can be further exploited in genome-wide RNAi screens for insulator components. It provides a useful tool that complements the transgenic and genetic approaches for studying this important class of regulatory elements.</p

Evaluation of Tourist Activities and Destination Attraction Capacity Using Geotags

Podatci o turističkim odredištima dostupni putem društvenih mreža nov su izvor informacija koji otvara velike mogućnosti proučavanja turističkih aktivnosti. Primjenom turističkih geooznaka iz društvene mreže VKontakte procijenili smo privlačnost turističkih odredišta oko Kavkaskih mineralnih voda. Na temelju podataka iz javnih profila turista koji objavljuju geooznake analizirali smo njihovu spolnu i dobnu strukturu, preferencije vezane uz godišnja doba te geografsko podrijetlo. Na primjeru odmarališta Pjatigorsk razvili smo klasifikaciju područja za rekreaciju za mjesno stanovništvo i turiste. Među osobama koje prikazuju svoje fotografije prevladavaju žene (64,7%). Godišnja doba u kojima se objavljuje najveći broj fotografija su proljeće i ljeto (64,8 % fotografija). Na turističkom odredištu Kavkaskih mineralnih voda geooznake unutar regije prevladavaju nad geooznakama iz drugih regija. Većina turista iz drugih regija dolazi iz Moskve, Sankt Peterburga, Rostova na Donu i Krasnodara. Stoga geooznake na fotografijama možemo primijeniti kao drugi izvor informacija za procjenu turističkih aktivnosti i privlačnosti tih odredišta.Tourist location data accessible through social networks is a new source of information which is opening up great opportunities for studying tourism activities. We used tourist geotags from the VKontakte social network to evaluate the attractiveness of tourist destinations around the Caucasian Mineral Waters. Based on the data received from the public profiles of tourists publishing geotags, we analyzed their gender- and age-related structure, seasonal preferences, and geographic origins. The resort of Pyatigorsk was used as a case study to develop a classification of recreational areas for local people and tourists. Women prevailed among those displaying their photos – 64.7%. The two major seasons for attaching photos were spring and summer – 64.8 % of the photos. The specific feature of the Caucasian Mineral Waters viewed as a tourist destination implied the prevalence of intraregional geotags over interregional ones. The major interregional tourist flow came from the cities of Moscow, St. Petersburg, Rostov-on-Don, and Krasnodar. The geotags on photos, therefore, may be used as an alternative source of information when evaluating the tourist activity and attractiveness of these destinations

Spatial analysis of the spread of Covid-19 and its demographic consequences in the regions of the South of European Russia

The article presents results of the multi-scale analysis of the processes of coronavirus infection spread and its impact on the demographic situation in the world, Russia and regions of the South of the European part of Russia. The methodological basis of the study was the principles of geoinformation monitoring, making it possible to process and visualize large volumes of diverse materials. The information base was statistical data from the Russian and foreign sources reflecting the spread of coronavirus infection at various spatial levels from global to regional-local. The characteristic features of changes in the parameters of the disease during its active expansion are described. The article also deals with dynamics in demographic indicators and identifies trends in their widespread deterioration. The contribution of the South of European Russia macro-region to the all-Russian Covid-19 situation is determined. Development of the coronavirus pandemic at the level of municipal districts is analyzed using individual regions as an example. The study identifies main factors of the Covid-19 pandemic development and demonstrates some of its features and consequences in the largest urban agglomerations

Epidermal Growth Factor Receptor and PTEN Modulate Tissue Factor Expression in Glioblastoma through JunD/Activator Protein-1 Transcriptional Activity

Hypoxia and necrosis are fundamental features of glioblastoma (GBM) and their emergence is critical for the rapid biological progression of this fatal tumor; yet, underlying mechanisms are poorly understood. We have suggested that vaso-occlusion following intravascular thrombosis could initiate or propagate hypoxia and necrosis in GBM. Tissue factor (TF), the main cellular initiator of coagulation, is overexpressed in GBMs and likely favors a thrombotic microenvironment. Epidermal growth factor receptor (EGFR) amplification and PTEN loss are two common genetic alterations seen in GBM but not in lower-grade astrocytomas that could be responsible for TF up-regulation. The most frequent EGFR mutation in GBM involves deletion of exons 2 to 7, resulting in the expression of a constitutively active receptor, EGFRvIII. Here, we show that overexpression of EGFR or EGFRvIII in human glioma cells causes increased basal TF expression and that stimulation of EGFR by its ligand, EGF, leads to a marked dose-dependent up-regulation of TF. In all cases, increased TF expression led to accelerated plasma coagulation in vitro. EGFR-mediated TF expression depended most strongly on activator protein-1 (AP-1) transcriptional activity and was associated with c-Jun NH2-terminal kinase (JNK) and JunD activation. Restoration of PTEN expression in PTEN-deficient GBM cells diminished EGFR-induced TF expression by inhibiting JunD/AP-1 transcriptional activity. PTEN mediated this effect by antagonizing phosphatidylinositol 3-kinase activity, which in turn attenuated both Akt and JNK activities. These mechanisms are likely at work in vivo, as EGFR expression was highly correlated with TF expression in human high-grade astrocytoma specimens

Planar fracturing and development of petroleum reservoirs

The research is relevant in the context of improvement of oil and gas recovery in low-permeability clastic reservoirs and shale. The main aim of the research is to substantiate the use of planar fracturing to improve oil and gas recovery in low-permeability clastic reservoirs. The research is based on a correlation between currently available theoretical developments on planar fracturing and practical data obtained with petroleum geology, lithology and seismology of upper Jurassic and lower Cretaceous formations in West Siberia Research methods: determination of the planar fracturing system with seismic survey (Prony's method), observation of the oriented core fracturing, lithologic and petrographic research of rock formations density zones, analysis of the reservoir production capacity in its expected zones of planar fracturing. Results. The author has analyzed Igolsk oilfield J1 2 reservoir development. The paper demonstrates the interconnection of the increased rates with rock formation density zones, determined by the seismic survey. The author correlated the density zones with the orientation of the planar fracturing system in upper Jurassic formations in West Siberia. The relation between the fracturing and the reservoir intervals of high clay content are introduced with lithology research data. The author compared the orientations of the global density field for Devonian-Carbon deposits in North America and the field of distribution of microseismic researches obtained at hydraulic fracturing of Low-Carbon shale formation Barnet. It was shown that the seismic activity area was formed by multiple regions of microseisms in northern-east and northern-west orientations which correspond to the predicted directions of rotating field global densities of the considered stratigraphic horizon in this region by the literary sources

Planar fracturing and development of petroleum reservoirs

The research is relevant in the context of improvement of oil and gas recovery in low-permeability clastic reservoirs and shale. The main aim of the research is to substantiate the use of planar fracturing to improve oil and gas recovery in low-permeability clastic reservoirs. The research is based on a correlation between currently available theoretical developments on planar fracturing and practical data obtained with petroleum geology, lithology and seismology of upper Jurassic and lower Cretaceous formations in West Siberia Research methods: determination of the planar fracturing system with seismic survey (Prony's method), observation of the oriented core fracturing, lithologic and petrographic research of rock formations density zones, analysis of the reservoir production capacity in its expected zones of planar fracturing. Results. The author has analyzed Igolsk oilfield J1 2 reservoir development. The paper demonstrates the interconnection of the increased rates with rock formation density zones, determined by the seismic survey. The author correlated the density zones with the orientation of the planar fracturing system in upper Jurassic formations in West Siberia. The relation between the fracturing and the reservoir intervals of high clay content are introduced with lithology research data. The author compared the orientations of the global density field for Devonian-Carbon deposits in North America and the field of distribution of microseismic researches obtained at hydraulic fracturing of Low-Carbon shale formation Barnet. It was shown that the seismic activity area was formed by multiple regions of microseisms in northern-east and northern-west orientations which correspond to the predicted directions of rotating field global densities of the considered stratigraphic horizon in this region by the literary sources

Studies of critical heat fluxes in small diameter channels

The paper presents the results of experimental studies of critical heat flows in vertical small diameter channels, when the coolant moves from bottom to top, which were carried out in the Obninsk branch of MEPhI in the 1970s of the last century but have not become widespread due to the lack of demand for their practical use. Nowadays, the interest in such works is manifested, first of all, in the development of compact plants and devices, particularly in nuclear power engineering. As a coolant, water, Freon-12 and 96% ethyl alcohol were used. High velocities of underheated liquid at high heat fluxes on the channel wall lead to the so-called “fast crisis” of heat transfer. In this case, the magnitude of the heat flux depends mainly on the parameters of the coolant flow in the wall zone rather than the flow core. The “slow crisis” is mainly observed at high vapor concentrations, relatively low mass flow rates and in an annular-dispersed flow. The value of the critical heat flow in this case depends mainly on the flow parameters in the core, which are probably close to the average coolant flow parameters. The conditions in the near-wall region are also largely determined by the flow in the core. High heat transfer coefficients in a flow moving at high speed usually result in a much smaller and slower rise in the wall temperature. Sometimes a DNB heat flux can occur bypassing the boiling process. In the core of a VVER-type reactor operating in its nominal mode, surface boiling is present in a number of fuel rods. Probably, surface boiling will also be present in transportable and small-size nuclear power plants. Therefore, an important task is to conduct relevant research in this area

Method and complex of express diagnostics of liquid packaged products

In order to improve the efficiency of quality control and safety of liquid packaged products, a method and model of an automated complex have been developed that allow rapid diagnostics of liquid without opening the container and sampling. The article describes the method, the results of its application in the identification of engine oil and the model of a portable automated complex with sensors-covers for continuous monitoring of bottled water. The data of experimental studies of the models are presented. The obvious result of using the method and the complex, from the point of view of energy saving and energy efficiency, is its application for continuous control of motor and transformer oils, to ensure the technical resource of motor vehicles and transformers at electrical substations, which, according to preliminary estimates, reduces the cost of selective control and losses from unscheduled repairs by 2 times