Induction of gene expression by stimulation of LPS-activated MPs with IFN-γ and IL-4

<p><b>Copyright information:</b></p><p>Taken from "Polarized monocyte response to cytokine stimulation"</p><p>Genome Biology 2005;6(2):R15-R15.</p><p>Published online 21 Jan 2005</p><p>PMCID:PMC551535.</p><p>Copyright © 2005 Nagorsen et al.; licensee BioMed Central Ltd.</p> MPs obtained from PBMC from five normal donors were stimulated for 4 h with one cytokine representative of the alternative (IL-4) and one of the classical group (IFN-γ) and gene transcription measured by TaqMan real-time PCR. The relative quantification of 10 genes was calculated by normalizing the ratio of the mean copy number for each gene with the mean copy number of the reference AFAP gene in MPs from five donors. Statistically significant differences (-value < 0.05) between the two cytokine treatments as assessed by Student's -test are represented by an asterisk