1 research outputs found
Electrophoretic Deposition of Gentamicin-Loaded Bioactive Glass/Chitosan Composite Coatings for Orthopaedic Implants
Despite their widespread application,
metallic orthopaedic prosthesis
failure still occurs because of lack of adequate bone-bonding and
the incidence of post-surgery infections. The goal of this research
was to develop multifunctional composite chitosan/Bioglass coatings
loaded with gentamicin antibiotic as a suitable strategy to improve
the surface properties of metallic implants. Electrophoretic deposition
(EPD) was applied as a single-step technology to simultaneously deposit
the biopolymer, bioactive glass particles, and the antibiotic on stainless
steel substrate. The microstructure and composition of the coatings
were characterized using SEM/EDX, XRD, FTIR, and TGA/DSC, respectively.
The in vitro bioactivity of the coatings was demonstrated by formation
of hydroxyapatite after immersion in simulated body fluid (SBF) in
a short period of 2 days. High-performance liquid chromatography (HPLC)
measurements indicated the release of 40% of the loaded gentamicin
in phosphate buffered saline (PBS) within the first 5 days. The developed
composite coating supported attachment and proliferation of MG-63
cells up to 10 days. Moreover, disc diffusion test showed improved
bactericidal effect of gentamicin-loaded composite coatings against <i>S. aureus</i> compared to control non-gentamicin-loaded coatings