Determination of factors affecting overall survival.

<p>Patients overall survival analyses were made between two groups: (A) patients with or without mutations in <i>NLRP</i> genes; (B) patients with or without mutations in <i>TP53</i> genes; (C) patients with tumors that harbored <i>TLR</i> mutations and those without <i>TLR</i> mutations; (D) patients with or without HPV infections; (E) patients with low stage (stage II) or advanced stage (stage III and beyond) tumors; (F) patients of less or more than 56 years old; (G) patients who received no adjuvant therapy, radiotherapy alone or both chemotherapy and radiotherapy.</p

Identification of <i>NLRP</i> mutations in FOM HNSCC.

<p>Mutations in four <i>NLRP</i> genes were identified in FOM HNSCC patients. Black triangles indicate novel mutations identified in this study. Gray triangles represent reported mutations in the Catalogue of Somatic Mutations in Cancer (COSMIC) database.</p

Mutation rates of FOM HNSCC.

<p>(A) Numbers of missense and nonsense mutations were compared between patients with FOM or non-FOM HNSCC. (B) Mutation rates were compared between patients with FOM or non-FOM HNSCC.</p

Mutation rates comparisons.

<p>(A) Numbers of missense and nonsense mutations were compared between tumors with or without <i>NLRP</i> mutations. (B) Numbers of missense and nonsense mutations were compared between tumors with or without <i>TP53</i> mutations. (C) Numbers of missense and nonsense mutations were compared between tumors with or without <i>TLR</i> genes mutations. (D) Mutation rates [shown as mutations per million base (MB) pairs] were compared between tumors with or without <i>NLRP</i> mutations. (E) Mutation rates [shown as mutations per million base (MB) pairs] were compared between tumors with or without <i>TP53</i> mutations. (F) Mutation rates [shown as mutations per million base (MB) pairs] were compared between tumors with or without <i>TLR</i> genes mutations. P value less than 0.05 was considered significant.</p

Demographic Profile of Study Subjects with Primary Conventional HNSCC (PC-SCC).

<p>Contingency tables were analyzed by Fisher's exact test. Survival distributions were analyzed by Log Rank test. Notes:</p>†<p>Typical number of alcohol drinks in 2 week period, §Mann-Whitney U test,</p>∥<p>Fisher's exact test,</p>¶<p>Log rank test,</p>*<p>Comparing patients with or without mutations in <i>NLRP</i> genes.</p

Identification of <i>NLRP</i> mutations in primary HNSCC.

<p>Novel mutations were identified in 10 <i>NLRP</i> genes in HNSCC. Most mutations were involving chromosomal locations 11p15.4 and 19q13.42-19q13.43.</p