Western blotting analysis of AR, P450arom, ERα and ERβ of pig sperm extracts

<p><b>Copyright information:</b></p><p>Taken from "Cytochrome P450arom, androgen and estrogen receptors in pig sperm"</p><p>http://www.rbej.com/content/5/1/23</p><p>Reproductive Biology and Endocrinology 2007;5():23-23.</p><p>Published online 6 Jun 2007</p><p>PMCID:PMC1894639.</p><p></p> Individual sperm samples: 1–3. LNCaP extracts were used as positive controls for AR and ERβ (C+) while MCF7 extracts were used as positive controls for P450 arom and ERα (C+). No bands in negative controls (C-). β actin served as loading control. Molecular weights (KD) were indicated on the right of the blot

Immunofluorescence labelling of androgen and estrogen receptors in pig spermatozoa: A) AR red fluorescence in sperm proximal mid-piece

<p><b>Copyright information:</b></p><p>Taken from "Cytochrome P450arom, androgen and estrogen receptors in pig sperm"</p><p>http://www.rbej.com/content/5/1/23</p><p>Reproductive Biology and Endocrinology 2007;5():23-23.</p><p>Published online 6 Jun 2007</p><p>PMCID:PMC1894639.</p><p></p> B) P450arom red brilliant light in the proximal tail of sperm with a diffuse labelling in the distal tail. C) ERα red fluorescence in the sperm mid-piece, together with a faint labelling in the tail. D) ERβ green intense light in the sperm acrosomal region. : immunonegative absorption controls. : 5 μm