49 research outputs found
Determination of Real-Time Efflux Phenotypes in Escherichia coli AcrB Binding Pocket Phenylalanine Mutants Using a 1,2′-Dinaphthylamine Efflux Assay
To evaluate the importance of phenylalanine residues for substrate transport in the Escherichia coli efflux pump protein AcrB, we subjected Phe-to-Ala binding pocket mutants to a real-time efflux assay with the novel near-infrared lipophilic membrane probe 1,2′-dinaphthylamine (1,2′-DNA). All mutations, with the exception of F617A, led to considerable retardation of efflux. F610A was the point mutation with the most pronounced impact, followed by F628A, F615A, F136A, and F178A. This is the first study to demonstrate the importance of single phenylalanine residues within the AcrB binding pocket for real-time substrate transport
An optimized DHPLC protocol for molecular testing of the **EXT1** and **EXT2** genes in hereditary multiple osteochondromas
The role of self-efficacy in the outcome of physiotherapy for urinary incontinence
PURPOSE: Perceived self-efficacy (S-E) (belief in one's ability to perform a behaviour) is a powerful mediator of health behaviours and outcomes in many areas (Bukelew et al 1996; Hellman 1997; Kores et al 1990; Rejeski et al, 1998). Physiotherapy for incontinence follows a self-management approach but evidence of the role of S-E is limited and conflicting. This study examined i) whether perceptions of S-E are related to outcome and ii) whether S-E beliefs change during treatment.RELEVANCE:Urinary incontinence is a common condition with adverse psychosocial consequences. Physiotherapy, including pelvic floor exercises and bladder training, is advocated as a first-line management. Understanding the role of S-E in physiotherapy outcome may enable therapists to modify treatment to enhance S-E and maximise success.SUBJECTS: 26 women (mean age 48.8 years) with stress and mixed urinary incontinence (median duration 4.75 years) referred to physiotherapy were recruited.METHODS AND MATERIALS: S-E was measured before and after treatment using the validated Generalised Self Efficacy Scale and the Incontinence Self-Efficacy and Outcome Expectancy Questionnaire, designed for the study. Physiotherapy outcome was determined after 6 weeks of self-management using validated measures (i.e. Digital Vaginal Assessment, the Symptom Severity Index, and the Kings Health Questionnaire) and compared to baseline measurements.ANALYSES: The analyses were conducted using SPSS. As the data were non-parametric and the sample size was small, Spearman's Rho was used to test associations between baseline self-efficacy and improvement in outcome measures and Wilcoxon's signed rank test was applied to changes in S-E scores.RESULTS: Incontinence improvement was noted for all measures. Significant correlations were found between improvement in muscle grade and initial pelvic floor self-efficacy (p= .03 r=.43) and outcome expectancy (p=.01 r= .54) and between improvement in symptom severity and generalised self-efficacy (p=.03 r=.42). No change in generalised self efficacy was seen during treatment (p=.60 z=-.53) but there were significant reductions in pelvic floor exercise self-efficacy (p=.020 z=-2.32) and outcome expectancy (p=.012 z=-2.52). Cautious interpretation is required however, because of multiple testing.CONCLUSIONS: Women with greater initial beliefs in their general coping abilities and ability to perform pelvic floor exercises experienced the greatest symptomatic improvement after 6 weeks physiotherapy. However, after 6 weeks treatment, women were significantly less positive about their ability to perform the exercises and the likely effectiveness of treatment. The findings suggest that current strategies may be failing to help patients maintain self-efficacy and a positive approach to outcome, which may adversely affect their maintenance of the exercise regime over a longer period
Nanoceria-promoted low Pd–Ni catalyst for the synthesis of secondary amines from aliphatic alcohols and ammonia
This paper describes the preparation of a series of bimetallic Pd–Ni catalysts supported over nanoceria with very low Ni and Pd loading (<0.5 wt%) for the direct amination of aliphatic alcohols with ammonia. Different metal impregnation methods were used for engineering the interaction between Pd, Ni and nanoceria. The as-prepared catalysts were characterized in detail by combining XRD, H2-TPR, H2-TPD, STEM-EDS-SDD and XPS. The sequence of impregnation of both metals and the Pd loading affected to an important extent the catalytic activity by conditioning the crystallite size and the Pd and Ni speciation, as well as the reducibility and reversible H2 storage properties. By optimizing the preparation protocols, a 0.5wt% Pd–0.5wt% Ni–Pd/CeO2 formulation prepared by sequential impregnation of the nickel and palladium precursors afforded 80% yield of dioctylamine at almost full conversion [TON = 1160 mmol per mmol (Ni + Pd)surface] in the direct amination reaction of 1-octanol with ammonia at 180 °C for 2 h. Metal leaching during the reaction could be completely avoided. The high catalytic performance of Pd–Ni induced by nanoceria places this catalyst among the best ever reported catalysts for the synthesis of secondary amines
Pregnancy after intracytoplasmic sperm injection of metaphase II-oocytes in a man with complete retrograde ejaculation
Behandeling van extreme oligoasthenoteratozoospermie d.m.v. intracytoplasmatische injectie van \ue9\ue9n spermatozo\uf6n in de eicel
Behandeling van extreme oligoasthenoteratozoospermie d.m.v. intracytoplasmatische injectie van \ue9\ue9n spermatozo\uf6n in de eicel
Sections: Editorial – The Resourceful Preacher”; Doctrinal; Devotional; Homiletical, including Sermon Outlines; Practical. Cover: The Editorial.https://digitalcommons.olivet.edu/cotn_pm/1144/thumbnail.jp