24 research outputs found
Multivalent Calixarene-Based Liposomes as Platforms for Gene and Drug Delivery
Get PDFThe formation of calixarene-based liposomes was investigated, and the characterization of
these nanostructures was carried out using several techniques. Four amphiphilic calixarenes were
used. The length of the hydrophobic chains attached to the lower rim as well as the nature of the
polar group present in the upper rim of the calixarenes were varied. The lipid bilayer was formed
with one calixarene and with the phospholipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine,
DOPE. The cytotoxicity of the liposomes for various cell lines was also studied. From the results
obtained, the liposomes formed with the least cytotoxic calixarene, (TEAC12)4
, were used as nanocarriers of both nucleic acids and the antineoplastic drug doxorubicin, DOX. Results showed that
(TEAC12)4/DOPE/p-EGFP-C1 lipoplexes, of a given composition, can transfect the genetic material,
although the transfection efficiency substantially increases in the presence of an additional amount of
DOPE as coadjuvant. On the other hand, the (TEAC12)4/DOPE liposomes present a high doxorubicin
encapsulation efficiency, and a slow controlled release, which could diminish the side effects of
the drugThis work was financed by the Consejer铆a de Conocimiento, Innovaci贸n y Universidades
de la Junta de Andaluc铆a (FQM-206, FQM-274, and PY20-01234), the VI Plan Propio Universidad de
Sevilla (PP2019/00000748), RTI2018-100692-B-100; P18-RT-1271; PI18-0005-2018; VI-PP AY.SUPLEM2019; RYC-2015-18670, The R+D+I grant PID2019-104195G from the Spanish Ministry of Science and
Innovation-Agencia Estatal de Investigaci贸n/10.13039/501100011033 (P.H.) and the European Union
(Feder Funds). The authors thank the University of Seville for the grant VPPI-US. J.A.L. also thanks
the Fundaci贸n ONCE funded by the Fondo Social Europe
The Use of Anhydrous Barium Hydroxide for Selective Alkylation of Dialkyloxy-tert-butyl-calix[4]arenes
No full textA practical method of selective alkylation of the third hydroxyl group of disubstituted tert-butyl-calix[4]arenes using anhydrous barium hydroxide as a base was developed in this study. The use of this method in the synthesis of inherently chiral derivatives is shown herein
The Use of Anhydrous Barium Hydroxide for Selective Alkylation of Dialkyloxy-<i>tert</i>-butyl-calix[4]arenes
No full textA practical method of selective alkylation of the third hydroxyl group of disubstituted tert-butyl-calix[4]arenes using anhydrous barium hydroxide as a base was developed in this study. The use of this method in the synthesis of inherently chiral derivatives is shown herein
