Photo-Protective Mechanisms and the Role of Poly (ADP-Ribose) Polymerase Activity in a Facultative CAM Plant Exposed to Long-Term Water Deprivation

The Crassulacean acid metabolism (CAM) pathway helps plants to alleviate the oxidative stress under drought, but the shift to CAM-idling may expose plants to the overproduction of reactive oxygen species causing cell damages. The facultative CAM species Portulacaria afra L., was subjected to long-term water deprivation to assess the photo-protective strategies and the poly (ADP-ribose) polymerase (PARP) activity during water stress and plant capability to recover from the stress. Measurements of titratable acidity, chlorophyll fluorescence emission, and antioxidant activity were performed during the stress and rewatering. Under water deprivation, plants shifted from C3 to CAM metabolism, reaching the CAM-idling status at the end of the stress period. The daily variation of the titratable acidity and PARP activity increased at the beginning of stress and declined with stress progression, reaching the lowest value at the end of stress treatment. H2 O2 content, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities increased with the severity of water stress. The photochemical processes remained high during the entire stress period indicating the presence of alternative sinks to CO2 fixation. The elevated activity of catalase under severe water stress suggests the occurrence of photorespiration in sustaining the photosynthetic electron transport under CAM-idling condition. The overall data indicate that scavenger enzymes, photorespiration and PARP activity modulation contribute to the strong resistance of P. afra to severe water stress, preserving the functioning of photosynthetic apparatus and ensuring plant recovery with rewatering

A low-luminosity type-1 QSO sample; III. Optical spectroscopic properties and activity classification

We report on the optical spectroscopic analysis of a sample of 99 low-luminosity quasi-stellar objects (LLQSOs) at $z\leq 0.06$ base the Hamburg/ESO QSO survey (HES). The LLQSOs presented here offer the possibility of studying the faint end of the QSO population at smaller cosmological distances and, therefore, in greater detail. A small number of our LLQSO present no broad component. Two sources show double broad components, whereas six comply with the classic NLS1 requirements. As expected in NLR of broad line AGNs, the [S{\sc{ii}}]$-$based electron density values range between 100 and 1000 N$_{e}$/cm$^{3}$. Using the optical characteristics of Populations A and B, we find that 50\% of our sources with H$\beta$ broad emission are consistent with the radio-quiet sources definition. The remaining sources could be interpreted as low-luminosity radio-loud quasar. The BPT-based classification renders an AGN/Seyfert activity between 50 to 60\%. For the remaining sources, the possible star burst contribution might control the LINER and HII classification. Finally, we discuss the aperture effect as responsible for the differences found between data sets, although variability in the BLR could play a significant role as well.Comment: 22 pages; 5 tables; 17 figures; in press with A&

Impact of communicative and informative strategies on influenza vaccination adherence and absenteeism from work of health care professionals working at the university hospital of palermo, Italy: A quasi-experimental field trial on twelve influenza seasons

Every year, about 20% of health care workers (HCWs) acquire influenza, continuing to work and encouraging virus spreading. Influenza vaccination coverage rates and absenteeism from work among HCWs of the University Hospital (UH) of Palermo were analyzed before and after the implementation of several initiatives in order to increase HCWs’ awareness about influenza vaccination. Vaccines administration within hospital units, dedicated web pages on social media and on the UH of Palermo institutional web site, and mandatory compilation of a dissent form for those HCWs who refused vaccination were carried out during the last four influenza seasons. After the introduction of these strategies, influenza vaccination coverage went up from 5.2% (2014/2015 season) to 37.2% (2018/2019 season) (p<0.001), and mean age of vaccinated HCWs significantly decreased from 48.1 years (95% CI: 45.7–50.5) to 35.9 years (95% CI: 35.0–36.8). A reduction of working days lost due to acute sickness among HCWs of the UH of Palermo was observed. Fear of adverse reactions and not considering themselves as a high-risk group for contracting influenza were the main reasons reported by HCWs that refused vaccination. Strategies undertaken at the UH of Palermo allowed a significant increase in vaccination adherence and a significant reduction of absenteeism from work

Curcumin affects HSP60 folding activity and levels in neuroblastoma cells

The fundamental challenge in fighting cancer is the development of protective agents able to interfere with the classical pathways of malignant transformation, such as extracellular matrix remodeling, epithelial\u2013mesenchymal transition and, alteration of protein homeostasis. In the tumors of the brain, proteotoxic stress represents one of the main triggering agents for cell transformation. Curcumin is a natural compound with anti-inflammatory and anti-cancer properties with promising potential for the development of therapeutic drugs for the treatment of cancer as well as neurodegenerative diseases. Among the mediators of cancer development, HSP60 is a key factor for the maintenance of protein homeostasis and cell survival. High HSP60 levels were correlated, in particular, with cancer development and progression, and for this reason, we investigated the ability of curcumin to affect HSP60 expression, localization, and post-translational modifications using a neuroblastoma cell line. We have also looked at the ability of curcumin to interfere with the HSP60/HSP10 folding machinery. The cells were treated with 6, 12.5, and 25 \ub5M of curcumin for 24 h, and the flow cytometry analysis showed that the compound induced apoptosis in a dose-dependent manner with a higher percentage of apoptotic cells at 25 \ub5M. This dose of curcumin-induced a decrease in HSP60 protein levels and an upregulation of HSP60 mRNA expression. Moreover, 25 \ub5M of curcumin reduced HSP60 ubiquitination and nitration, and the chaperonin levels were higher in the culture media compared with the untreated cells. Furthermore, curcumin at the same dose was able to favor HSP60 folding activity. The reduction of HSP60 levels, together with the increase in its folding activity and the secretion in the media led to the supposition that curcumin might interfere with cancer progression with a protective mechanism involving the chaperonin

Improved Torsion Pendulum for Ground Testing of LISA Displacement Sensors

We discuss a new torsion pendulum design for ground testing of prototype LISA (Laser Interferometer Space Antenna) displacement sensors. This new design is directly sensitive to net forces and therefore provides a more representative test of the noisy forces and parasitic stiffnesses acting on the test mass as compared to previous ground-based experiments. We also discuss a specific application to the measurement of thermal gradient effects.Comment: 4 pages 1 figure, to appear in the Proceedings of the 10th Marcel Grossmann Meeting on General Relativit

Developing an evidence-based program sustainability training curriculum: A group randomized, multi-phase approach

Abstract Background The emergence of dissemination and implementation (D&I) science has driven a rapid increase in studies of how new scientific discoveries are translated and developed into evidence-based programs and policies. However, D&I science has paid much less attention to what happens to programs once they have been implemented. Public health programs can only deliver benefits if they reach maturity and sustain activities over time. In order to achieve the full benefits of significant investment in public health research and program development, there must be an understanding of the factors that relate to sustainability to inform development of tools and trainings to support strategic long-term program sustainability. Tobacco control programs, specifically, vary in their abilities to support and sustain themselves over time. As of 2018, most states still do not meet the CDC-recommended level for funding their TC program, allowing tobacco use to remain the leading cause of preventable disease and death in the USA. The purpose of this study is to empirically develop, test, and disseminate training programs to improve the sustainability of evidence-based state tobacco control programs and thus, tobacco-related health outcomes. Methods This paper describes the methods of a group randomized, multi-phase study that evaluates the empirically developed “Program Sustainability Action Planning Training” and technical assistance in US state-level tobacco control programs. Phase 1 includes developing the sustainability action planning training curriculum and technical assistance protocol and developing measures to assess long-term program sustainability. Phase 2 includes a group randomized trial to test the effectiveness of the training and technical assistance in improving sustainability outcomes in 24 state tobacco control programs (12 intervention, 12 comparison). Phase 3 includes the active dissemination of final training curricula materials to a broader public health audience. Discussion Empirical evidence has established that program sustainability can improve through training and technical assistance; however, to our knowledge, no evidence-based sustainability training curriculum program exists. Therefore, systematic methods are needed to develop, test, and disseminate a training that improves the sustainability of evidence-based programs. Trial registration NCT03598114. Registered 25 July 2018—retrospectively registered

Trends of influenza B during the 2010–2016 seasons in 2 regions of north and south Italy: The impact of the vaccine mismatch on influenza immunisation strategy

Influenza A and B viruses are responsible for respiratory infections, representing globally seasonal threats to human health. The 2 viral types often co-circulate and influenza B plays an important role in the spread of infection. A 6-year retrospective surveillance study was conducted between 2010 and 2016 in 2 large administrative regions of Italy, located in the north (Liguria) and in the south (Sicily) of the country, to describe the burden and epidemiology of both B/Victoria and B/Yamagata lineages in different healthcare settings. Influenza B viruses were detected in 5 of 6 seasonal outbreaks, exceeding influenza A during the season 2012–2013. Most of influenza B infections were found in children aged ≤ 14 y and significant differences were observed in the age-groups infected by the different lineages. B/Victoria strains prevailed in younger population than B/Yamagata, but also were more frequently found in the community setting. Conversely, B/Yamagata viruses were prevalent among hospitalized cases suggesting their potential role in the development of more severe disease. The relative proportions of viral lineages varied from year to year, resulting in different lineage-level mismatch for the B component of trivalent influenza vaccine. Our findings confirmed the need for continuous virological surveillance of seasonal epidemics and bring attention to the adoption of universal influenza immunization program in the childhood. The use of tetravalent vaccine formulations may be useful to improve the prevention and control of the influenza burden in general population

Evaluation of the burden of HPV-related hospitalizations as a useful tool to increase awareness: 2007–2017 data from the sicilian hospital discharge records

In light of the implementation of human papillomavirus (HPV) prevention strategies, epidemiological studies in different geographical areas are required in order to assess the impact of HPV-related diseases. The purpose of the present study was to describe the burden of HPV-related hospitalizations in Sicily. A retrospective observational study estimated 43,531 hospitalizations attributable to HPV from 2007 to 2017. During the observed period, there was a decrease for all HPV-related conditions with a higher reduction, among neoplasms, for cervical cancer (annual percent change (APC) = −9.9%, p < 0.001). The median age for cervical cancer was 45 years old, with an increasing value from 43 to 47 years (p < 0.001). The age classes with greater decreases in hospital admissions for invasive cancers were women aged 35 years or more (APC range from −5.5 to −9.86) and 25–34 years old (APC = −11.87, p < 0.001) for women with cervical carcinoma in situ. After ten years for vaccine introduction and sixteen years for cervical cancer screening availability, a relatively large decrease in hospital admissions for cervical cancer and other HPV-related diseases in Sicily was observed. Some clinical characteristics of hospitalization, such as increasing age, are suggestive clues for the impact of preventive strategies, but further research is needed to confirm this relationship

Acceleration disturbances and requirements for ASTROD I

ASTRODynamical Space Test of Relativity using Optical Devices I (ASTROD I) mainly aims at testing relativistic gravity and measuring the solar-system parameters with high precision, by carrying out laser ranging between a spacecraft in a solar orbit and ground stations. In order to achieve these goals, the magnitude of the total acceleration disturbance of the proof mass has to be less than 10−13 m s−2 Hz−1/2 at 0.1 m Hz. In this paper, we give a preliminary overview of the sources and magnitude of acceleration disturbances that could arise in the ASTROD I proof mass. Based on the estimates of the acceleration disturbances and by assuming a simple controlloop model, we infer requirements for ASTROD I. Our estimates show that most of the requirements for ASTROD I can be relaxed in comparison with Laser Interferometer Space Antenna (LISA).Comment: 19 pages, two figures, accepted for publication by Class. Quantum Grav. (at press