4 research outputs found
NMR structure of a DNA duplex containing the modified nucleotide 3'-<em>C</em>-(hydroxymethyl)thymidine
Universal Biotin–PEG-Linked Gold Nanoparticle Probes for the Simultaneous Detection of Nucleic Acids and Proteins
Novel
biotin–polyethylene glycol (biotin–PEG) gold
nanoparticle probes have been synthesized and used as universal constructs
for the detection of protein (prostate-specific antigen, PSA) and
nucleic acid targets (microRNAs) from a single sample. Microarray
assays based upon these probes enabled sensitive detection of biomarker
targets (50 fM for nucleic acid targets and 1 pg/ÎĽL for the
PSA target). Ways of detecting biomarkers, including nucleic acids
and proteins, are necessary for the clinical diagnosis of many diseases,
but currently available diagnostic platforms rely primarily on the
independent detection of proteins or nucleic acids. In addition to
the economic benefits associated with the use of a single platform
to detect both classes of analytes, studies have shown that the simultaneous
identification of multiple classes of biomarkers in the same sample
could be useful for the detection and management of early stage diseases,
especially when sample amounts are limited. Therefore, these new probes
and the assays based upon them open the door for high-sensitivity
combination-target assays for studying and tracking biological pathways
and diseases