Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium pv. campestris-0

Ernatant of pv campestris strain B100 at a 30 k fold magnification (OMVs highlighted by arrows). (B) Immunogold electron microscopy of 1% (w/v) uranyl acetate stained cell at 14 k fold magnification after incubation with an anti-B100 antibody which was detected by a 10 nm gold particle bound to a goat anti-rabbit antibody (gold-labelled OMV highlighted by arrows). (C) Immunogold electron micrograph of 1% (w/v) uranyl acetate stained OMVs at 27 k fold magnification after incubation with an anti-B100 antibody detected with a 10 nm gold particle bound to an goat anti-rabbit antibody.<p><b>Copyright information:</b></p><p>Taken from "Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium pv. campestris"</p><p>http://www.biomedcentral.com/1471-2180/8/87</p><p>BMC Microbiology 2008;8():87-87.</p><p>Published online 2 Jun 2008</p><p>PMCID:PMC2438364.</p><p></p

Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium pv. campestris-2

He brackets with numeration indicate the sections taken from the gel for tryptic digestion. (B) Protein profiles of OMVs isolated from cultures grown in M9 medium and the outer membrane (OM) fraction prepared from the same culture.<p><b>Copyright information:</b></p><p>Taken from "Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium pv. campestris"</p><p>http://www.biomedcentral.com/1471-2180/8/87</p><p>BMC Microbiology 2008;8():87-87.</p><p>Published online 2 Jun 2008</p><p>PMCID:PMC2438364.</p><p></p

Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium pv. campestris-3

Lar location or function. The pie charts indicate the number of proteins in each group isolated from OMVs collected from M9 medium (A) from XVM2 medium (B) and the outer membrane proteins isolated from cells grown in M9 medium (C). The percentage values within the pie chart give the amount of proteins grouped as virulence-associated, which comprise the groups of virulence factors and type III secretion proteins.<p><b>Copyright information:</b></p><p>Taken from "Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium pv. campestris"</p><p>http://www.biomedcentral.com/1471-2180/8/87</p><p>BMC Microbiology 2008;8():87-87.</p><p>Published online 2 Jun 2008</p><p>PMCID:PMC2438364.</p><p></p