78 research outputs found
Synchrotron imaging of dentition provides insights into the biology of Hesperornis and Ichthyornis, the "last" toothed birds.
BACKGROUND: The dentitions of extinct organisms can provide pivotal information regarding their phylogenetic position, as well as paleobiology, diet, development, and growth. Extant birds are edentulous (toothless), but their closest relatives among stem birds, the Cretaceous Hesperornithiformes and Ichthyornithiformes, retained teeth. Despite their significant phylogenetic position immediately outside the avian crown group, the dentitions of these taxa have never been studied in detail. To obtain new insight into the biology of these 'last' toothed birds, we use cutting-edge visualisation techniques to describe their dentitions at unprecedented levels of detail, in particular propagation phase contrast x-ray synchrotron microtomography at high-resolution. RESULTS: Among other characteristics of tooth shape, growth, attachment, implantation, replacement, and dental tissue microstructures, revealed by these analyses, we find that tooth morphology and ornamentation differ greatly between the Hesperornithiformes and Ichthyornithiformes. We also highlight the first Old World, and youngest record of the major Mesozoic clade Ichthyornithiformes. Both taxa exhibit extremely thin and simple enamel. The extension rate of Hesperornis tooth dentine appears relatively high compared to non-avian dinosaurs. Root attachment is found for the first time to be fully thecodont via gomphosis in both taxa, but in Hesperornis secondary evolution led to teeth implantation in a groove, at least locally without a periodontal ligament. Dental replacement is shown to be lingual via a resorption pit in the root, in both taxa. CONCLUSIONS: Our results allow comparison with other archosaurs and also mammals, with implications regarding dental character evolution across amniotes. Some dental features of the 'last' toothed birds can be interpreted as functional adaptations related to diet and mode of predation, while others appear to be products of their peculiar phylogenetic heritage. The autapomorphic Hesperornis groove might have favoured firmer root attachment. These observations highlight complexity in the evolutionary history of tooth reduction in the avian lineage and also clarify alleged avian dental characteristics in the frame of a long-standing debate on bird origins. Finally, new hypotheses emerge that will possibly be tested by further analyses of avian teeth, for instance regarding dental replacement rates, or simplification and thinning of enamel throughout the course of early avian evolution
Phenolic and furanic compounds of Portuguese chestnut and French, American and Portuguese oak wood chips
Botanical species used on aging process must
be wisely and judiciously chosen, and for this selection, a
basic knowledge of the chemical composition of woods is
warranted. Aiming to contribute to extend the knowledge
of the chemical composition of several wood species useful
for enological purposes, we have focused our studies on
Portuguese chestnut and French, American and Portuguese
oak chips. The profile of low molecular weight phenolic
composition of these chips was achieved, using an optimized
extraction method based on pressurized liquid
extraction, followed by the quantification of phenolic acids,
phenolic aldehydes and furanic derivatives by high-performance
liquid chromatography (HPLC-DAD). The
identification of those compounds was also confirmed by
LC-DAD/ESI-MS. This study allowed the determination of
the low molecular phenolic composition of Portuguese
chestnut and French, American and Portuguese oak wood.
According to our results, the influence of the botanical
species seems to be more relevant than the geographic
origin of the wood species
Extrinsic Fluorescent Dyes as Tools for Protein Characterization
Noncovalent, extrinsic fluorescent dyes are applied in various fields of protein analysis, e.g. to characterize folding intermediates, measure surface hydrophobicity, and detect aggregation or fibrillation. The main underlying mechanisms, which explain the fluorescence properties of many extrinsic dyes, are solvent relaxation processes and (twisted) intramolecular charge transfer reactions, which are affected by the environment and by interactions of the dyes with proteins. In recent time, the use of extrinsic fluorescent dyes such as ANS, Bis-ANS, Nile Red, Thioflavin T and others has increased, because of their versatility, sensitivity and suitability for high-throughput screening. The intention of this review is to give an overview of available extrinsic dyes, explain their spectral properties, and show illustrative examples of their various applications in protein characterization
Evolutionary Trends of the Pharyngeal Dentition in Cypriniformes (Actinopterygii: Ostariophysi)
International audienceBACKGROUND: The fish order Cypriniformes is one of the most diverse ray-finned fish groups in the world with more than 3000 recognized species. Cypriniformes are characterized by a striking distribution of their dentition: namely the absence of oral teeth and presence of pharyngeal teeth on the last gill arch (fifth ceratobranchial). Despite this limited localisation, the diversity of tooth patterns in Cypriniformes is astonishing. Here we provide a further description of this diversity using X-ray microtomography and we map the resulting dental characters on a phylogenetic tree to explore evolutionary trends. RESULTS: We performed a pilot survey of dental formulae and individual tooth shapes in 34 adult species of Cypriniformes by X-ray microtomography (using either conventional X-ray machine, or synchrotron microtomography when necessary) or by dissecting. By mapping morphological results in a phylogenetic tree, it emerges that the two super-families Cobitoidea and Cyprinoidea have followed two distinct evolutionary pathways. Furthermore, our analysis supports the hypothesis of a three-row dentition as ancestral for Cyprinoidea and a general trend in tooth row reduction in most derived lineages. Yet, this general scheme must be considered with caution as several events of tooth row gain and loss have occurred during evolutionary history of Cyprinoidea. SIGNIFICANCE: Dentition diversity in Cypriniformes constitutes an excellent model to study the evolution of complex morphological structures. This morphological survey clearly advocates for extending the use of X-ray microtomography to study tooth morphology in Cypriniformes. Yet, our survey also underlines that improved knowledge of Cypriniformes life traits, such as feeding habits, is required as current knowledge is not sufficient to conclude on the link between diet and dental morphology
Fluorimetric nitrite analysis using 2,3-diaminonaphthalene: an improvement of the method
One of the fluorimetric methods for nitrite determination is based on the reaction of nitrite ions with
2,3-diaminonaphthalene (DAN) to form fluorescent 1-[H]-naphthotriazole. The procedure is sensitive, simple
and rapid. However, the DAN reagent is carcinogenic and its use in a large excess for the reaction might
be a drawback for an extensive application. In particular to know if a stoechiometric quantity of the
reagent might be sufficient to run the reaction, the method was re-examined. In fact, using the latter
condition, it was still possible to get nitrite determination in the range 0 to 50 g
NO.L, with a rather smaller analysis time
Identification of N-methyl-1-(1,3-benzodioxol-5-yl)-2-butanamine (MBDB), an homologue derivative of "ecstasy"
Some of the various N-substituted derivatives of the MDA have become popular drugs of abuse (MDMA, MDEA) in Europe and in France. With the increasing seizures of
MBDB, [N-methyl-1-(1,3-benzodioxol-5-yl)-2-butanamine] a new homologue of MDMA and MDEA, forensic analysis of these controlled substances requires the use of powerful
techniques to characterize them. Analysis by either high performance liquid chromatography (HPLC) or capillary gas chromatography (CGC) allows the separation of MDMA, MDEA,
MBDB and EBDB. The isomers MDEA and MBDB are best differentiated by either nuclear magnetic resonance spectroscopy (1H-NMR) or the combination of mass spectra (MS) and
retention time (CGC). These techniques were successfully applied to seized tablets
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