Correlation of Gene Over and Under-Expression and Severity of Illness Score (SIS).

<p>Statistical correlation (Pearson, Spearman, Pearson Log Gene and Pearson Log-Log) of severity of illness score and quantitative gene expression was significant for five genes. <b>A)</b> RETN (resistin gene), SORT 1 (sortilin 1 gene) and P2RX1 (purinergic receptor P2X, ligand-gated ion channel 1) showed over-expression. <b>B)</b> LOC641788 (similar to CDC26 subunit of anaphase promoting complex) and STAT 4 (signal transducer and activator of transcription 4) showed under-expression. Of those genes, RETN and STAT 4 had the strongest correlation coefficients (RETN: R2 = 0.8 and STAT 4: R2 = –0.83).</p

Gene Expression Biosignature in Whole Blood From Healthy Controls and Patients With MRSA AHO.

<p>Complete statistical analysis (Welch t-Test with Benjamini - Hochberg false discovery rate, p<0.05) between the two groups (healthy control group with 9 subjects and MRSA AHO group with 10 subjects) identified 58 genes (24 over-expressed and 34 under-expressed) which significantly differentiated children with MRSA AHO from the healthy controls.</p

Module Analysis of Gene Expression Profile in Whole Blood From Patients with MRSA AHO.

<p>Gene expression in one of the subjects within the healthy control group revealed an unusual array of cellular expression. This subject was excluded and microarray statistical analysis was repeated without his information. Gene expression levels were compared between healthy controls and patients with MRSA AHO on a module by module analysis. Each column represents a subject and each row a cell type. Colored spots represent the percentage of significant over-expressed (red) or under expressed (blue) transcripts (Mann-Whitney p<0.05). The intensity of the color refers to the number of transcripts within each of the pre-selected modules not the actual fold change that is up or down regulated compared to healthy controls. Modular analysis identified that patients had upregulation of innate immunity and mild downregulation of adaptive immunity.</p

Module Analysis of Gene List Applied To an Independent Set of Patients Revealed The Same Modular Map.

<p>Average modular transcriptional fingerprint of patients with MRSA AHO showed that patients had upregulation of innate immunity including platelet, neutrophil and erythroid-related genes. Analysis also showed that patients had mild downregulation of adaptive immunity including B- cell, T-cell and NK cell-related genes. Interferon signature was not noted in this analysis.</p

Severity of Illness Score (SIS) Calculation for Children Hospitalized with MRSA AHO During Study Time Period.

<p>Severity of Illness Score (SIS) Calculation for Children Hospitalized with MRSA AHO During Study Time Period.</p

Top 24 Over-expressed and 34 Under-expressed Genes in MRSA AHO.

<p>Genes associated to SIS in bold.</p

IVIg therapy and gene expression analysis.

<p>Eight additional CVID subjects were tested before and 5-7 days after receiving IVIg. Individual transcripts (567) were selected by Welch ANOVA test (MTC: Benjamini and Hochberg, FDR, p-cutoff <0.01) between healthy controls, pre-IVIg and post-IVIg patients.</p

Gene expression analysis of CVID blood (test set) compared to healthy controls.

<p>Unsupervised clustering of 11,559 RNA transcripts in whole blood cells, selected by Present At least Once (PALO) differentiates CVID patients from healthy controls in the test set.</p

Production of IFN-α.

<p>For IFN-α production, PBMCs from control subjects (10) and CVID subjects with (+) and without inflammatory (-) complications, were stimulated with increasing amounts of loxoribine. IFN-α in supernatants was assessed by ELISA.</p