Immunohistochemical staining of Der p 1 using mouse anti-Der p 1.

<p>Immunohistochemical staining of Der p 1 using mouse anti-Der p 1.</p

Immunohistochemical detection of Der p 1, IgE and CD68, and the presence of eosinophil leukocytes in nasal biopsies before and after <i>in vivo</i> challenge with Der p 1 allergen.

<p>H-E staining for Eosinophils; see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0127477#sec006" target="_blank">Methods</a>.</p><p>*Double staining with Der p 1.</p><p>** Staining of cells and the mucous in the epithelium, lamina propria and the submucosal glands. An ordinal scale was used for intensity of Der p 1 staining. This allows for rank order (negative, 1+, 2+, 3+, 4+), but does not allow for measuring relative degree of difference between them.</p><p>***Comparison between non-challenged (pre) and challenged (post) tissue.</p><p>****Comparison between atopic and nonatopic (non-challenged tissue).</p><p>EP: nasal mucosal epithelium; LP: lamina propria; GL: subepithelial mucous glands.</p><p>Number of cells: Mean (range); NA: not available; Statistics: t-test was used for comparison between means.</p><p>Immunohistochemical detection of Der p 1, IgE and CD68, and the presence of eosinophil leukocytes in nasal biopsies before and after <i>in vivo</i> challenge with Der p 1 allergen.</p

Total recovery of Der p 1 after allergen challenge in 20 nonatopic subjects.

<p>*Each subject had two random sampling times</p><p>**Samples taken on one occasion only.</p><p>***Total allergen recovery—comparison between sampling times (p = 0.2; analysis of variance, Friedman test for multiple samples)</p><p>**** Total allergen recovery—comparison between aqueous or particulate at any time point (p≥0.2; Wilcoxon rank sum test)</p><p>N = Number of subjects; IQR = Interquartile Range</p><p>Total recovery of Der p 1 after allergen challenge in 20 nonatopic subjects.</p