Not Available

Not AvailableA commentary on the stress-responsive miRNAs of soybeanNot Availabl

Not Available

Not AvailableDrought is a major reason for reduced productivity in principal food crops including soybean. Development of drought tolerant varieties is imperative to tide over erratic rainfall conditions in Indian context. Here we studied molecular mechanism governing drought tolerance in Indian soybean cultivar NRC7 to identify drought responsive genic biomarkers. Drought associated physiological parameters like relative water content (RWC) and electrolytic leakage studies under water-deficit condition revealed very low water status in the stressed plants. In addition, membrane damage studies on electrolytic leakages revealed that the stressed plants exhibited greater membrane damage than control plants. Differential display RT-PCR (DD-RT-PCR) identified two drought responsive transcripts (gmDRT1 and gmDRT2) from the soybean cultivar NRC7. The identified transcripts were found to have sequence homology with dehydrin protein and ion ATPase transporters. These biomarkers identified for drought tolerance would also help in engineering drought tolerant soybean cultivars.Not Availabl

Not Available

Not AvailableMungbean yellow mosaic India virus (MYMIV) infecting soybean and other legumes causes yellow mosaic disease (YMD). Evaluation of soybean genotypes for YMD resistance involves field screening at disease hot spots or in a protected environment using infectious clones or viruliferous whiteflies as sources of virus inocula. Development of efficient virus inoculation and quantification protocols to screen soybean genetic stocks against YMD is imperative for breeding resistant varieties. Binary plasmids harbouring complete, tandem dimeric genomic components DNA A and DNA B of MYMIV-soybean isolate were engineered. The infectivity of the clones was demonstrated in soybean genotypes JS335 and UPSM534 that display contrasting YMD resistance. As a follow-up, soybean germplasm lines, breeding lines, and representative cultivars that were initially screened at an YMD hot-spot were then subjected to Agrobacterium-based infection with MYMIV. Quantitative real time polymerase chain reaction (qRT-PCR) based copy number analysis of MYMIV genomic components allowed soybean genotypes to be classified into three discrete categories; resistant, moderately resistant and susceptible to the viral infection. Thus, a soybean germplasm disease screening system based on agro-infection and qRT-PCR based quantification of MYMIV was developed to facilitate breeding YMD resistant soybean. The implications of this study for obtaining YMD resistant soybean cultivars are discussed.Not Availabl

Not Available

The following sections are included: Introduction The C3MP Methodology and Participation Protocols The C3MP Database and Evaluation C3MP Phase 1: Lessons Learned and Initial Findings Continuing C3MP Efforts and Future Work Frequently Asked Questions (FAQs) ReferencesThe following sections are included: Introduction The C3MP Methodology and Participation Protocols The C3MP Database and Evaluation C3MP Phase 1: Lessons Learned and Initial Findings Continuing C3MP Efforts and Future Work Frequently Asked Questions (FAQs) ReferencesNot Availabl

Not Available

The following sections are included: Introduction The C3MP Methodology and Participation Protocols The C3MP Database and Evaluation C3MP Phase 1: Lessons Learned and Initial Findings Continuing C3MP Efforts and Future Work Frequently Asked Questions (FAQs) ReferencesThe following sections are included: Introduction The C3MP Methodology and Participation Protocols The C3MP Database and Evaluation C3MP Phase 1: Lessons Learned and Initial Findings Continuing C3MP Efforts and Future Work Frequently Asked Questions (FAQs) ReferencesNot Availabl