89 research outputs found

    Antibiotic Resistance in Staphylococcus aureus and Coagulase Negative Staphylococci Isolated from Goats with Subclinical Mastitis

    Get PDF
    Antimicrobial resistance patterns and gene coding for methicillin resistance (mecA) were determined in 25 S. aureus and 75 Coagulase Negative Staphylococci (CNS) strains isolates from half-udder milk samples collected from goats with subclinical mastitis. Fourteen (56.0%) S. aureus and thirty-one (41.3%) CNS isolates were resistant to one or more antimicrobial agents. S. aureus showed the highest resistance rate against kanamycin (28.0%), oxytetracycline (16.0%), and ampicillin (12.0%). The CNS tested were more frequently resistant to ampicillin (36.0%) and kanamycin (6.7%). Multiple antimicrobial resistance was observed in eight isolates, and one Staphylococcus epidermidis was found to be resistant to six antibiotics. The mecA gene was not found in any of the tested isolates. Single resistance against β-lactamics or aminoglicosides is the most common trait observed while multiresistance is less frequent

    A Survey on aflatoxin M<sub>1</sub> content in sheep and goat milk produced in Sardinia region, Italy (2005-2013)

    Get PDF
    In the present work the results of a survey conducted in Sardinia Region on Aflatoxin M1 (AFM1) contamination in milk of small ruminants from 2005 to 2013 are reported. A total of 517 sheep and 88 goat milk samples from bulk tank, tank trucks and silo tank milk were collected. Analyses were performed by the Regional Farmers Association laboratory using high-performance liquid chromatography following the ISO 14501:1998 standard. None of the sheep milk samples analysed during 2005- 2012 showed AFM1 contamination. In sheep milk samples collected in 2013, 8 out of 172 (4.6%) were contaminated by AFM1 with a concentration (mean&#177;SD) of 12.59&#177;14.05 ng/L. In one bulk tank milk sample 58.82 ng/L AFM1 was detected, exceeding the EU limit. In none of goat milk samples analysed from 2010 to 2012 AFM1 was detected. In 2013, 9 out of 66 goat milk samples (13.6%) showed an AFM1 concentration of 47.21&#177;19.58 ng/L. Two of these samples exceeded the EU limit, with concentrations of 62.09 and 138.6 ng/L. Higher contamination frequency and concentration rates were detected in bulk tank milk samples collected at farm than in bulk milk truck or silo samples, showing a dilution effect on AFM1 milk content along small ruminants supply chain. The rate and levels of AFM1 contamination in sheep and goat milk samples were lower than other countries. However, the small number of milk samples analysed for AFM1 in Sardinia Region in 2005-2013 give evidence that food business operators check programmes should be improved to ensure an adequate monitoring of AFM1 contamination in small ruminant dairy chain

    Linking water stock in Mediterranean temporary ponds with hydrological balance at landscape scale

    Get PDF
    Water stock in Mediterranean Temporary ponds results from the hydorological balance between direct or indirect rainwater supply (inflow) and losses in the atmosphere, overflow, infiltration (outflow). Losses into the atmosphere are due either to evapotranspiration, which includes evaporation from open water, soil and canopy dampened by the rain and plant transpiration. Our research was aimed to relate the hydrological balance evaluated at landscape scale with the presence and size of over 100 ponds using historical series of meteorological data and satellite images freely available online. The study area is located in Sardinia, on a 44 km2 basaltic plateau, 550 m a.s.l, named Giara di Gesturi

    Perceptions of Present and Future Climate Change Impacts on Water Availability for Agricultural Systems in the Western Mediterranean Region

    Get PDF
    Many Mediterranean countries have experienced water shortages during the last 20 years and future climate change projections foresee further pressure on water resources. This will have significant implications for irrigation water management in agricultural systems in the future. Through qualitative and quantitative empirical research methods carried out on a case study on four Mediterranean farming systems located in Oristano, Italy, we sought to understand the relationship between farmers' perceptions of climate change (i.e., increased temperature and decreased precipitation) and of present and future water availability for agriculture as forecasted by climatic and crop models. We also explored asymmetries between farmers' perceptions and present and future climate change and water scenarios as well as factors influencing perceptions. Our hypotheses were that farmers' perceptions are the main drivers of actual water management practices and that sustainable practices can emerge from learning spaces designed from the understanding of the gaps between perceptions and scientific evidences. Results showed that most farmers perceived that climate change is occurring or will occur in their area. They also perceived that there has been an increased temperature trend, but also increased precipitation. Therefore, they are convinced that they have and will have enough irrigation water for agriculture in the near future, while climate change projections foresee an increasing pressure on water resources in the Mediterranean region. Such results suggest the need for (i) irrigation management policies that take into account farmers' perceptions in order to promote virtuous behaviors and improve irrigation water use efficiency; (ii) new, well-designed learning spaces to improve the understanding on climate change expectations in the near future in order to support effective adaptive responses at the farm and catchment scales

    Postnatal growth in a cohort of Sardinian intrauterine growth-restricted infants

    Get PDF
    Recent studies have shown that infants with intrauterine growth restriction (IUGR) undergo catch-up growth during infancy. The aim of our study was to evaluate the postnatal growth in a cohort of IUGR infants born in a tertiary-level Obstetric University Hospital of Northern Sardinia. An observational retrospective study was conducted on 12 IUGR (group A) and 12 control infants (group B) by measuring the anthropometric parameters of weight (W), length (L) and head circumference (HC) from birth to the 3rd postnatal year. At birth, significant differences were found between group A and group B with regard to all the auxological parameters (W, mean 1846.6 versus 3170.8 g, p &lt; 0.0001; HC, 30.1 versus 34.4 cm, p &lt; 0.0001; L, mean 43.4 versus 49.4 cm, p &lt; 0.0001). During the 1st year, 8 of 12 (70%) IUGR infants exhibited a significant catch-up growth in the 3 anthropometric parameters and a regular growth until the 3rd year of follow-up. The majority but not all infants born with IUGR in our series showed significant postnatal catch-up growth essentially during the first 12 months of life. An improved knowledge of the causes of IUGR will help to develop measures for its prevention and individualized treatment

    Abnormal Circadian Modification of A\u3b4-Fiber Pathway Excitability in Idiopathic Restless Legs Syndrome

    Get PDF
    Restless legs syndrome (RLS) is characterized by unpleasant sensations generally localized to legs, associated with an urge to move. A likely pathogenetic mechanism is a central dopaminergic dysfunction. The exact role of pain system is unclear. The purpose of the study was to investigate the nociceptive pathways in idiopathic RLS patients. We enrolled 11 patients (mean age 53.2\u2009\ub1\u200919.7 years; 7 men) suffering from severe, primary RLS. We recorded scalp laser-evoked potentials (LEPs) to stimulation of different sites (hands and feet) and during two different time conditions (daytime and nighttime). Finally, we compared the results with a matched control group of healthy subjects. The A\u3b4 responses obtained from patients did not differ from those recorded from control subjects. However, the N1 and the N2-P2 amplitudes' night/day ratios after foot stimulation were increased in patients, as compared to controls (N1: patients: 133.91\u2009\ub1\u200950.42%; controls: 83.74\u2009\ub1\u200934.45%; p = 0.016; A\u3b4-N2-P2: patients: 119.15\u2009\ub1\u200915.56%; controls: 88.42\u2009\ub1\u200923.41%; p = 0.003). These results suggest that RLS patients present circadian modifications in the pain system, which are not present in healthy controls. Both sensory-discriminative and affective-emotional components of pain experience show parallel changes. This study confirms the structural integrity of A\u3b4 nociceptive system in idiopathic RLS, but it also suggests that RLS patients present circadian modifications in the pain system. These findings could potentially help clinicians and contribute to identify new therapeutic approaches

    Current and future effects of global change on a hotspot's freshwater diversity

    Get PDF
    Deforestation, climate change and invasive species constitute three global threats to biodiversity that act synergistically. However, drivers and rates of loss of freshwater biodiversity now and in the future are poorly understood. Here we focus on the potential impacts of global change on freshwater mussels (Order Unionida) in Sundaland (SE Asia), a vulnerable group facing global declines and recognized indicators of overall freshwater biodiversity. We used an ensemble of distribution models to identify habitats potentially suitable for freshwater mussels and their change under a range of climate, deforestation and invasion scenarios. Our data and models revealed that, at present, Sundaland features 47 and 32 Mha of habitat that can be considered environmentally suitable for native and invasive freshwater mussels, respectively. We anticipate that by 2050, the area suitable for palm oil cultivation may expand between 8 and 44 Mha, representing an annual increase of 2–11%. This is expected to result in a 20% decrease in suitable habitat for native mussels, a drop that reaches 30% by 2050 when considering concomitant climate change. In contrast, the habitat potentially suitable for invasive mussels may increase by 44–56% under 2050 future scenarios. Consequently, native mussels may compete for habitat, food resources and fish hosts with invasive mussels across approximately 60% of their suitable range. Our projections can be used to guide future expeditions to monitor the conservation status of freshwater biodiversity, and potentially reveal populations of endemic species on the brink of extinction. Future conservation measures—most importantly the designation of nature reserves—should take into account trends in freshwater biodiversity generally, and particularly species such as freshwater mussels, vital to safeguard fundamental ecosystem services.This study is funded by the Malaysian Ministry of Higher Education (Project FRGS/1/2015/WAB13/UNIM//1). BG was supported by a research fellowship funded by the Spanish Program of R + D + I (JCI2012-11908)

    Assessment of land-use and land-cover changes from 1965 to 2014 in Tam Giang-Cau Hai Lagoon, central Vietnam

    No full text
    This study integrates the use of multi-source and multi-resolution remote sensing, topographic and fieldbased datasets to quantify land-use and land-cover (LULC) changes along a coastal stretch of Thua Thien Hue Province (central Vietnam). The LULC change analysis involves the Tam Giang-Cau Hai lagoon, the largest lagoon system in Southeast Asia, which is running nearly 70 km along the coast and having about 22,000 ha of water surface. The LULC change analysis was performed by computer-aided visual interpretation for 5 years (1965, 1989, 2000, 2006 and 2014) using satellite imagery from LANDSAT MSS, TM, ETMþ and 8, ASTER and SPOT5. National topographic maps were also used for the 1965 and 2000 years. To adequately represent the LULC features and peculiarities of central Vietnam coastal areas, an adapted CORINE Land Cover nomenclature was used where new 3rd and 4th level classes were adopted. Due to their intrinsic relative high spatial and radiometric resolution, SPOT5 images from 2006 were assumed as a reference for interpretation keys and first delineation. Changes were mapped by editing those vectors representing features which underwent LULC change prior or after 2006. Spatial and temporal changes were analyzed by post-classification approach and validated by ground truth information. High detail object-based classification was finally performed to infer the capability of medium spatial resolution imagery for extracting cadastral scale pond maps. The accuracy of classification was checked by a polygon by polygon comparison with an existing aquaculture facility inventory. Five LULC maps were obtained by applying a legend of 21 classes including two newly defined: “Aquaculture ponds” and “Mangrove forest”. The overall classification accuracy of the LULC map is 85% while the KHAT statistics 0.81 for the year 2006. Accuracy of the object-based aquaculture facilities classification is 84% or better for the SPOT5 imagery and 47.9% for the ASTER imagery. The study provides a synoptic LULC representation for the largest lagoon system of Southeast Asia and delivers quantitative estimates of main changes occurred during the last 50 years. Moreover, it reveals the adaptability of the CORINE Land Cover method outside European environment. Finally, SPOT5 provides good results to map aquaculture features at cadastral scale, even if in some circumstances (e.g. tidal areas), the integration with higher spatial resolution multispectral sensors should be envisaged.</br
    corecore