Determination of Letrozole in Tablet Formulations by Reversed Phase High Performance Liquid Chromatography

Purpose: To develop a simple, rapid, accurate and cost-effective reversed phase high performance liquid chromatography (RP-HPLC) method for letrozole in bulk and in tablets. Methods: Development of a method for the determination of letrozole, an anti-cancer drug, by RPHPLC was undertaken using a new mobile phase of acetonitrile:water (50:50, v/v). The eluent was monitored at 265 nm. Results: The optimized conditions developed showed a linear response from 160 to 240 μg/mL, with a correlation coefficient (R2) of 0.999. The limit of detection (LOD) and limit of quantification ( LOQ) were 136 and 160 μg/mL, respectively. The assay values for the two branded letrozole tablets tested were 99.2 and 100.2 %, respectively with % relative standard deviation (RSD) of 0.781 and 0.568, respectively. The bench top stability data of the drug in the mobile phase indicate that the drug was stable in the mobile phase for 24 h. Recovery data were good. Placebo study for specificity and interference of common excipients showed that the method was specific and free from interfering substances. Conclusion: Therefore, the fully validated method developed was sensitive enough to carry out routine analysis of letrozole in tablet formulations with regard to its run time, simplicity of sample preparation and accuracy.Key words: Letrozole, Assay, HPLC, Validation, Tablet formulation

The Impact of Avatar Realism and Eye Gaze Control on Perceived Quality of Communication in a Shared Immersive Virtual Environment

This paper presents an experiment designed to investigate the impact of visual and behavioral realism in avatars on perceived quality of communication in an immersive virtual environment

UV Spectrophotometric Method for the Estimation of Valacyclovir HCl in Tablet Dosage Form

A simple, sensitive, highly accurate UV spectrophotometric method has been developed for the determination of valacyclovir in bulk and tablet dosage form. Solution of valacyclovir in 0.1N HCl shows maximum absorbance at 255 nm. Beer’s law was obeyed in the concentration range of 5-25 mcg mL-1 with 1.0910x104 mol-1 cm-1, the slope, intercept, correlation coefficient, detection and quantitation limits were also calculated. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablets dosage forms. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The percentages assay of valacyclovir HCl in tablet was 99.82%. The method was validated by determining its sensitivity, accuracy and precision which proves suitability of the developed method for the routine estimation of valacyclovir in bulk and solid dosage form