Immunoblot analysis of Sorcin, Cofilin-1, Apolipoprotein-A1 (Apo-A1) and Ran GTP-binding nuclear protein (Ran) in fertile women.

<p>Expression of Sorcin, Cofilin-1, Apo-A1 and Ran were checked in early-secretory (LH+2) and mid-secretory (LH+7) phase endometrium of fertile women. Representative images (upper panel) of immunoblot of Sorcin, Cofilin-1, Apo-A1 and Ran have been shown. β-actin served as a loading control for normalization. Quantification of band intensity (lower panel) was performed by densitometric analysis by using Quantity One software (v. 4.5.1) and a Gel Doc imaging system (Bio-Rad). p-values are (b) p<0.01, (c) p<0.05 and (d) p>0.05 versus early-secretory phase (LH+2). Values are expressed as mean± SE; n = 4, ES = early-secretory phase, MS = mid-secretory phase, LH+2 = 2 days after luteinizing hormone surge, LH+7 = 7 days after luteinizing hormone surge.</p

Various differentially expressed proteins.

<p>Bar diagram showing 9 differentially expressed proteins between early-secretory (LH+2) and mid-secretory (LH+7) phase endometrium from women with unexplained infertility. Values were expressed as mean ± SE, n = 8. P values are (b) p<0.01 and (c) p<0.05 versus early-secretory phase (LH+2). ES = early-secretory phase, MS = mid-secretory phase, LH+2 = 2 days after luteinizing hormone surge, LH+7 = 7 days after luteinizing hormone surge.</p

Immunohistochemical localization of Sorcin, Cofilin-1, Apolipoprotein-A1 (Apo-A1) and Ran GTP-binding nuclear protein (Ran) in infertile women.

<p>Representative images (upper panel) showing immunohistochemical localization of of Sorcin, Cofilin-1, Apo-A1 and Ran in early-secretory (LH+2) and mid-secretory (LH+7) phase endometrium from women with unexplained infertility. Lower panel shows image analysis of Sorcin, Cofilin-1, Apo-A1 and Ran in early-secretory and mid-secretory phase endometrium of infertile women. Staining intensity of all these proteins were quantified by image analysis software Image-Pro Plus 4.0 (Maryland, USA). Anti-mouse/anti-goat IgG was used as negative control and shown in inset (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111687#pone-0111687-g004" target="_blank">Fig. 4 A, E, I, M</a>). Magnification X 400, bar = 25 µm, p-values are (a) p<0.001, (b) p<0.01 and (c) p<0.05 versus early-secretory phase (LH+2). Values are expressed as mean ± SE. n = 4 in all the groups. S = stroma, GE = Glandular epithelium, LE = Luminal epithelium.</p

Validation of Sorcin, Cofilin-1, Apolipoprotein-A1 (Apo-A1) and Ran GTP-binding nuclear protein (Ran) in infertile women.

<p>Expression of Sorcin, Cofilin-1, Apo-A1 and Ran were analyzed by western blot analysis in early-secretory (LH+2) and mid-secretory phase (LH+7) endometrium of infertile women, which had not been previously analyzed in the 2D-PAGE analysis. The results matched the findings of 2D-PAGE analysis. Representative images (upper panel) of immunoblot of Sorcin, Cofilin-1, Apo-A1 and Ran have been shown. β-actin served as a loading control for normalization. Quantification of band intensity (lower panel) was performed by densitometric analysis by using Quantity One software (v. 4.5.1) and a Gel Doc imaging system (Bio-Rad). P values are (b) p<0.01 and (c) p<0.05 versus early-secretory phase (LH+2). Values are expressed as mean± SE; n = 4. ES = early-secretory phase, MS = mid-secretory phase, LH+2 = 2 days after luteinizing hormone surge, LH+7 = 7 days after luteinizing hormone surge.</p

Details of differentially expressed proteins identified by LC-MS analysis and comparison thereof with previous reports.

<p>Alteration in endometrial proteins during early- and mid-secretory phases of the cycle in women with unexplained infertility.</p><p>Details of differentially expressed proteins identified by LC-MS analysis and comparison thereof with previous reports.</p

2D-PAGE of early-secretory (LH+2) and mid-secretory (LH+7) phase endometrium of women with unexplained infertility.

<p>Representative gel images are shown here. The first dimension was performed by IEF on IPG strips over a range of pI 3–10, the second dimension on 12.5% SDS-PAGE gels and the proteins were visualized by silver staining. 9 differentially altered protein spots were identified by image master 2D platinum software and number denotes spot ID (0–23). LH+2 = 2 days after luteinizing hormone surge, LH+7 = 7 days after luteinizing hormone surge.</p

El defensor : Diario Liberal independiente, protector de los intereses materiales y morales de esta provincia: Año II Epoca 2 Número 113 - 1904 julio 11

Copia digital. Madrid : Ministerio de Cultura. Subdirección General de Coordinación Bibliotecaria, 200

Additional file 1: of Improving performance of the Tariff Method for assigning causes of death to verbal autopsies

List of causes of death of PHMRC. (DOCX 16 kb

Additional file 1: of A shortened verbal autopsy instrument for use in routine mortality surveillance systems

Gold standard clinical diagnosis criteria. (DOCX 117 kb