8 research outputs found
Apollonius and Callimachus on Heracles and Theiodamas: A Metapoetical Interpretation
Apollonius of Rhodes’ digression on Heracles and Theiodamas (Arg. 1.1211-20) alludes to Callimachus’ version of the story in his Aetia (fr. 24-5 Pf.). This article provides a metapoetical interpretation of the intertextual contact. The ways in which both poets deal with Heracles reveal the similar but different reactions of these “Callimachean” poets to the heroic-epic literary tradition
Additional file 4: of Anti-aging potential of extracts from Sclerocarya birrea (A. Rich.) Hochst and its chemical profiling by UPLC-Q-TOF-MS
Negative mode BPI chromatogram of quinic acid pure standard overlaid with that of Marula stem ethanol extract. A comparison of the retention time of quinic acid pure standard with that of peak 1 identified a quinic acid in Marula stem ethanol extract. (PPTX 85Â kb
Additional file 11: of Anti-aging potential of extracts from Sclerocarya birrea (A. Rich.) Hochst and its chemical profiling by UPLC-Q-TOF-MS
MS/MS fragmentation pattern of epicatechin gallate pure standard overlaid with MS/MS fragmentation of peak 7. A comparison of the MS/MS fragmentation pattern of epicatechin gallate pure standard to that of peak 7 identified as epicatechin gallate in Marula stem ethanol extract. (PPTX 84Â kb
Bioassay-Guided Investigation of the Tanzanian Plant <i>Pyrenacantha kaurabassana</i> for Potential Anti-HIV-Active Compounds
Two new anti-HIV xanthones, 6,7,11-trihydroxy-10-methoxy-9-(7-methoxy-3-methyl-1-oxoisochroman-5-yl)-2-methyl-12-oxo-12<i>H</i>-benzo[<i>b</i>]xanthene-4-carboxylic acid (<b>1</b>) and 6,7-dihydroxy-10,11-dimethoxy-9-(7-methoxy-3-methyl-1-oxoisochroman-5-yl)-2-methyl-12-oxo-12<i>H</i>-benzo[<i>b</i>]xanthene-4-carboxylic acid (<b>2</b>), and a new hexadecahydrochrysen-3-ol (<b>3</b>) were
isolated from the tubers of <i>Pyrenacantha kaurabassana</i>. Compounds <b>1</b> and <b>2</b> showed moderate anti-HIV
activity when tested in the deCIPhR<sup></sup> assay on HIV virus
type NL4-3, with IC<sub>50</sub> values of 21 and 2 μg/mL, respectively
Protective Roles of Monsonia angustifolia and Its Active Compounds in Experimental Models of Alzheimer’s Disease
Alzheimer’s
disease (AD), a progressive neurodegenerative
disorder, is characterized by the accumulation of neurotoxic β-amyloid
(Aβ) peptides, which consequently affects cognitive decline
and memory impairment. Current research on AD treatment is actively
focusing on the prevention of neurotoxic Aβ peptide accumulation. Monsonia angustifolia is reported to be consumed
as an indigenous vegetable in Tanzania. In this study, we investigated
the effect of the ethanol (EtOH) extract of M. angustifolia dried ground material on Aβ production and spatial learning
ability as protection against AD. The formation of Aβ peptides
was significantly reduced in HeLa cells stably transfected with the
Swedish mutant form of β-amyloid precursor protein (APPsw) after
treatment with a 60% EtOH extract of M. angustifolia. We next examined the cognitive-improving effects of the EtOH extract
in vivo. Tg2576 mice were treated with extract for 6 months and subjected
to Morris water maze and novel object recognition tests. The results
showed that the 60% EtOH extract of M. angustifolia significantly ameliorated behavioral deficits of the AD transgenic
mice and reduced the level of insoluble Aβ42 in the cerebral
cortex and hippocampus. We further found that the 60% EtOH extract
was effective for memory function recovery after shorter treatment
(4 months). In addition, we isolated and identified several single
compounds, justicidin A, 5-methoxyjusticidin A, chinensinaphthol,
retrochinensinaphthol methyl ether, and suchilactone, from M. angustifolia and tested these compounds.
Among them, justicidin A potently decreased the formation of Aβ
in APPsw-transfected cells. These data suggest that the 60% EtOH extract
of M. angustifolia has the potential
to be developed as a treatment of AD. Furthermore, justicidin A may
contribute, at least partially, to the Aβ alteration observed
with the extract treatment
DataSheet1_An in vitro study to elucidate the effects of Product Nkabinde on immune response in peripheral blood mononuclear cells of healthy donors.pdf
Introduction: A significant number of the South African population still rely on traditional medicines (TM) for their primary healthcare. However, little to no scientific data is available on the effects of most TM products on cytokine and cellular biomarkers of the immune response. We evaluated the impact of a TM [Product Nkabinde (PN)] in inducing cellular and cytokine biomarkers of immune response in peripheral blood mononuclear cells (PBMCs).Methods: PN, a combination of four indigenous South African plants was used in this study. The IC50 was established using the cell viability assay over 24 h. Luminex and flow cytometry assays were used to measure cytokine and cellular levels in PBMCs stimulated with PN and/or PHA over 24, 48, and 72 h, respectively. UPLC-HRMS was used to analyze an ethanol: water extract of PN to better understand the possible active compounds.Results: The IC50 concentration of PN in treated PBMCs was established at 325.3 μg/mL. In the cellular activation assay, the percentages of CD38-HLA-DR + on total CD4+ T cells were significantly increased in PBMCs stimulated with PN compared to unstimulated controls after 24 h (p = 0.008). PN significantly induced the production of anti-inflammatory IL-10 (p = Conclusion: The results show that PN possesses in vitro immunomodulatory properties that may influence immune and inflammatory responses. This study contributes to scientific knowledge about the immune effects of TM. More studies using PN are needed to further understand key parameters mediating induction, expression, and regulation of the immune response in the context of pathogen-associated infections.</p
Table1_An in vitro study to elucidate the effects of Product Nkabinde on immune response in peripheral blood mononuclear cells of healthy donors.xlsx
Introduction: A significant number of the South African population still rely on traditional medicines (TM) for their primary healthcare. However, little to no scientific data is available on the effects of most TM products on cytokine and cellular biomarkers of the immune response. We evaluated the impact of a TM [Product Nkabinde (PN)] in inducing cellular and cytokine biomarkers of immune response in peripheral blood mononuclear cells (PBMCs).Methods: PN, a combination of four indigenous South African plants was used in this study. The IC50 was established using the cell viability assay over 24 h. Luminex and flow cytometry assays were used to measure cytokine and cellular levels in PBMCs stimulated with PN and/or PHA over 24, 48, and 72 h, respectively. UPLC-HRMS was used to analyze an ethanol: water extract of PN to better understand the possible active compounds.Results: The IC50 concentration of PN in treated PBMCs was established at 325.3 μg/mL. In the cellular activation assay, the percentages of CD38-HLA-DR + on total CD4+ T cells were significantly increased in PBMCs stimulated with PN compared to unstimulated controls after 24 h (p = 0.008). PN significantly induced the production of anti-inflammatory IL-10 (p = Conclusion: The results show that PN possesses in vitro immunomodulatory properties that may influence immune and inflammatory responses. This study contributes to scientific knowledge about the immune effects of TM. More studies using PN are needed to further understand key parameters mediating induction, expression, and regulation of the immune response in the context of pathogen-associated infections.</p
Table2_An in vitro study to elucidate the effects of Product Nkabinde on immune response in peripheral blood mononuclear cells of healthy donors.xlsx
Introduction: A significant number of the South African population still rely on traditional medicines (TM) for their primary healthcare. However, little to no scientific data is available on the effects of most TM products on cytokine and cellular biomarkers of the immune response. We evaluated the impact of a TM [Product Nkabinde (PN)] in inducing cellular and cytokine biomarkers of immune response in peripheral blood mononuclear cells (PBMCs).Methods: PN, a combination of four indigenous South African plants was used in this study. The IC50 was established using the cell viability assay over 24 h. Luminex and flow cytometry assays were used to measure cytokine and cellular levels in PBMCs stimulated with PN and/or PHA over 24, 48, and 72 h, respectively. UPLC-HRMS was used to analyze an ethanol: water extract of PN to better understand the possible active compounds.Results: The IC50 concentration of PN in treated PBMCs was established at 325.3 μg/mL. In the cellular activation assay, the percentages of CD38-HLA-DR + on total CD4+ T cells were significantly increased in PBMCs stimulated with PN compared to unstimulated controls after 24 h (p = 0.008). PN significantly induced the production of anti-inflammatory IL-10 (p = Conclusion: The results show that PN possesses in vitro immunomodulatory properties that may influence immune and inflammatory responses. This study contributes to scientific knowledge about the immune effects of TM. More studies using PN are needed to further understand key parameters mediating induction, expression, and regulation of the immune response in the context of pathogen-associated infections.</p