3 research outputs found
Time to address quality control processes applied to antibody testing for infectious diseases
As testing for infectious diseases moves from manual, biological testing such as complement fixation to high throughput automated autoanalyzer, the methods for controlling these assays have also changed to reflect those used in clinical chemistry. However, there are many differences between infectious disease serology and clinical chemistry testing, and these differences have not been considered when applying traditional quality control methods to serology. Infectious disease serology, which is highly regulated, detects antibodies of varying classes and to multiple and different antigens that change according to the organisms' genotype/serotype and stage of disease. Although the tests report a numerical value (usually signal to cut-off), they are not measuring an amount of antibodies, but the intensity of binding within the test system. All serology assays experience lot-to-lot variation, making the use of quality control methods used in clinical chemistry inappropriate. In many jurisdictions, the use of the manufacturer-provided kit controls is mandatory to validate the test run. Use of third-party controls, which are highly recommended by ISO 15189 and the World Health Organization, must be manufactured in a manner whereby they have minimal lot-to-lot variation and at a level where they detect exceptional variation. This paper outlines the differences between clinical chemistry and infectious disease serology and offers a range of recommendations when addressing the quality control of infectious disease serology
Response to Tony Badrick regarding "Letter to the Editor regarding the article by Wayne J. Dimech et al. Time to address quality control processes applied to antibody testing for infectious diseases. Clin Chem Lab Med 2023; 61(2): 205-212 by"
Response to Tony Badrick regarding "Letter to the Editor regarding the article by Wayne J. Dimech et al. Time to address quality control processes applied to antibody testing for infectious diseases
Progressi in viticoltura
Obiettivo del libro \ue8 di fornire gli aggiornamenti pi\uf9 recenti delle basi genetiche, fisiologiche, biochimiche e degli aspetti pratici inerenti la coltivazione della vite. Stiamo assistendo in viticoltura ad un rapidissimo progresso delle conoscenze, in cui le innovazioni devono integrarsi nella tradizione millenaria della coltivazione della vite. L\u2019ampia e recente letteratura scientifica citata, gli argomenti pi\uf9 moderni trattati, l\u2019autorevolezza degli autori coinvolti, fanno di questo volume il compendio dei risultati della ricerca orientata a comprendere i numerosi e spesso controversi fenomeni che si osservano in vigneto. Nel contesto dei cambiamenti climatici, argomento cui \ue8 dedicato uno dei primi capitoli, tutto ci\uf2 che era consolidato in termini di conoscenze muta radicalmente e l\u2019odierna sfida \ue8 di essere quanto pi\uf9 preparati a aggiornare le scelte operative per il raggiungimento degli obiettivi di elevata qualit\ue0 delle produzioni vitivinicole. Il libro \ue8 focalizzato sulla viticoltura mediterranea, con le sue variet\ue0 di vite in grado di tollerare condizioni di stress abiotici e quindi di adattarsi al riscaldamento globale e alla siccit\ue0