An ontology-based consultation system to support medical care on board seagoing vessels

Background: A realistic possibility to obtain medical care for patients located in remote sites such as seagoing vessels, in which health professionals are not available, is to contact a doctor via telecommunication systems. In general, the medical knowledge of who on board ships is in charge of medical care is quite limited and therefore, in a first level telemedical consultation, the flow of information should be correct and its efficiency should be maximised. This paper describes an application conceived to improve requests of medical assistance from sailing ships. The ultimate objective of this system is a) to standardise as much as possible the requests of medical advice at a distance, b) to overcome language barriers and jammed-related troubles that could make difficult or not understandable a telephone conversation. Materials and methods: The application is based on a software engine extracting data from an ontological knowledgebase built ad hoc using Protégé. Results: Compared to the conventional consultation systems based on telephone and e-mail, the proposed device is more accurate and complete in terms of information contained in the request of assistance. Moreover, data received by the medical centre can be more easily managed, as they can be standardised. Conclusions: The system described here allows people responsible of medical care on board ships to forward detailed requests of assistance containing symptom-guided information on patient clinical conditions. This may represent an innovative tool for medical consultations at distance allowing the remote centre to provide more precise and quicker medical advice.

An improved characterization of horse (Equus caballus, 2n=64) chromosomes by using replicating G and R banding patterns

Abstract Peripheral blood lymphocytes were cultured and treated for early- and late-BrdU incorporation to perform replicating G- and R-banding patterns, respectively. Slides were treated for GBG-, RBA- and RBG-banding techniques. Improved banded karyotypes at early- (350 bands) and pro-metaphase (500 bands) stage were performed and GBG- and RBA-banded prometaphase karyotypes were presented for the first time on this species. All chromosomes, including the small acrocentrics, show clear and distinguishable G- and R-banding patterns. Chromosome identification followed the latest chromosome standard nomenclature (ISCNH 1997). This study is also our contribution to further standard karyotype attempts at the prometaphase stage

Characterization of telomere length in Agerolese cattle breed, correlating blood and milk samples

Studies into telomere length in cattle are relatively recent and have focused mainly on the Holstein Friesian cattle breed, making it arduous to evaluate the correlation with ageing due to the early age of culling in this breed. Telomere length provides information about the productive lifespan and the quality of farm management, complying with the 'One Health' approach. This study evaluated telomere length in Agerolese cattle, an autochthonous dairy breed characterized by a long productive lifespan (13 years). Multiplex quantitative PCR estimated telomere length in DNA extracted from blood and milk matrices. Interestingly, the results showed longer telomeres in Agerolese (compared to the Holstein Friesian cattle control group), with a negative correlation between telomere length and increasing age and a synchronous trend between blood and milk samples, with a positive correlation between them

Biometrics measurements in red foxes (Vulpes vulpes) in the Picentini area. Part I: intersexual differences between young adult and adult subjects.

A series of biometrics measurements have been obtained in 16 Red foxes (Vulpes vulpes) duly hunted in the Picentini area, outside the protected area. Data recorded included the sex, bodyweight, and age class (young adults 2 y.o.) as determined on tooth wearing by an experienced wildlife operator. Occipito-coccygeal (OC), nasal-occipitalis (NO), tail and total lengths, wither height, ear and hind foot (HF) length, upper and lower canine length and width (UCL, LCL, UCW, LCW) and carnassial length and width (data not shown), were measured directly on the carcasses. Condilo-basal (CB), hard palate (HP), scapular, mandibular and upper dentition (UD) lengths, inter-carnassial (IC) and zygomatic (Z) width were measured on latero-lateral and ventro-dorsal radiograms. A Wilcoxon sum rank test was applied to compare variables not normally distributed. For normally distributed variables, homoscedasticity was investigated by using a Levene’s test, post hoc a Student’s t test or a Welch ANOVA was applied. Significance was set at P<0.05. Results are summarized in table 1

Casting light on the European anchovy: from biology to conservation and industry

This manuscript explores the role of European anchovies (Engraulis encrasicolus) in the central Mediterranean Region, shedding light on their ecological significance, conservation challenges, and sustainable utilization. The European anchovy is one of Europe’s most important fish resources in the Mediterranean basin, and it is considered a keystone species, playing a pivotal role in both ecological and socio-economic dimensions. However, in recent decades, European anchovy, together with Sardina pilchardus (commonly known as European sardine), has suffered a population decline for several reasons. Consequently, it is necessary to improve the management of anchovy fisheries by understanding the reproductive modes and characteristics, the influence of currents on the passive transport of eggs and larvae, the feeding habits, the environmental adaptability (e.g., salinity), and the distribution of ecotypes along the Italian coasts. Such information is fundamental for the management of fisheries, especially artisanal ones, and to control frauds, especially in protected, geographically referred, and traditional high-quality commercial products. Various aspects, ranging from their population dynamics to their industrial processing and the ecological implications of these activities were delved, highlighting the knowledge about anchovy populations and ecotypes and its importance in maintaining ecosystem balance and sustaining human communities. The ecological interaction of anchovies within the food web, as essential data in the conservation actions and management of these resources was emphasized. In addition, the metabolic and stomach contents diversity among anchovy populations and ecotypes was discussed, enhancing our understanding of their adaptability to varying environmental conditions. The manuscript then explores the traditional and industrial processing of anchovies, encompassing aspects ranging from fishing techniques (i.e., methods of capture) to their industrial significance, sustainability concerns, issues of fraud, and the establishment of geographical traceability. Finally, the opportunities for sustainable and biotechnological utilization of anchovy discards were also further explored, demonstrating the potential for waste reduction and resource optimization