Surveillance and evidence of contamination in hospital environment from meticillin and vancomycin-resistant microbial agents

Introduction. Direct contact is undoubtedly the main means of transmission of hospital infections. An investigative study was therefore conducted to assess workplace surfaces at risk from microbial contamination. Methods. The study was conducted using swabs and contact slides placed on the palms of healthcare workers during their routine patient care and on workplace surfaces (e.g. telephones, computers, medication trolleys, taps) in treatment rooms, oper- ating theatres and wards. Disposable swabs were used for rapid screening and read with a bioluminometer. At the same time, a sample was taken from those testing positive using a contact slide. The samples testing positive for Staphylococci underwent identification to assess resistance to meticillin-resistant Sta- phylococcus aureus (MRS/MRSA) and to vancomycin (VISA/ VRSA) Results. Meticillin-resistant Staphylococcus strains were found on 14.7% (20/136) of samples taken from the hands of workers and 35.7% (15/42) of those from hospital surfaces. An even higher resistance to meticillin and/or vancomycin than that found for S. aureus was identified in nosocomial strains of coagulase negative staphylococci, including S. epidermidis and S. haemolyticus. Conclusion. The study concludes that there is thus a need for greater care in complying with procedures designed and support for surveillance to reduce the risk of infection

Health literacy in Mediterranean general population

INTRODUCTION Health literacy refers to "the ability to obtain, process, and understand basic health information and access health services in order to make informed choices." In essence, being able to acquire, understand, and use information for one's own health. METHODS Observational study through the administration of a face-to-face questionnaire conducted between July and September 2020 on 260 individuals residing between Calabria and Sicily, aged between 18 and 89 years. Questions related to education, lifestyle (alcohol, smoking, and physical activity). Multiple-choice questions to assess health literacy and conceptual skills, ability to find information on health topics and services, use of preventive medicine especially vaccinations, and ability to make decisions about one's own health. RESULTS Of 260, 43% were male and 57% female. The most represented age group is between 50 and 59 years. Forty-eight percent of respondents had a high school diploma. 39% smoke and 32% habitually consume alcoholic beverages; only 40% engage in physical activity. Ten percent had a low level of health literacy, average 55%, adequate 35%. CONCLUSIONS Given the importance of adequate HL on health choices and on individual and public wellbeing, it is essential to expand the knowledge of the individual, through public and private information campaigns and with an increasing involvement of family physicians, who are fundamental in training and informing their patients

Study on hepatitis B and C serologic status among municipal solid waste workers in Messina (Italy)

A study on hepatitis B and C virus seroprevalence was carried out on blood samples from 327 municipal solid waste workers in Messina (Italy) to verify the hypothesis that this category of workers is at high risk for such diseases. The fact that 32.41% of all the subjects showed previous exposure to hepatitis B virus (HBV) substantiates the need to proceed with compulsory HBV vaccine prophylaxis in this category of workers, also in anticipation of possible medical legal litigations

Identification of the microorganisms responsible for periodontopathy by Multiplex RT-PCR

The aim of our research was to identify by bacterial genomicDNA analysis the prevalence of five different species of periodontopathogenic bacteria present in the subgingival biofilm,specifically: Actinobacillus actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Bacterioides forsytus (Bf), Treponema denticola (Td).For the analysis we used the systematic Multiplex-PCR-microdentkit with species-specific primers. We studied a group of 48 subjects, 18 males and 30 females, from 18 to 78 years of age. Theinitial clinical screening enabled us to select, among the groupanalysed, 24 subjects with signs of active periodontopathy (GroupA) and 24 patients without identifiable clinical evidence of the disease used as the control group (Group B). Within the two experimental groups (A and B), the test was found to be positive in 75%of subjects from group A, whereas the test was found to be negative in all the subjects from group B. Our research shows thatthe Multiplex-PCR system is reliable. Furthermore, the sensitivity and simplicity of this technique, as well as the decrease inworking times and the possibility of identifying non-culturablebacteria, since the presence of viable organisms is not essential,make this technique indicated for the simultaneous identificationof periodontopathogenic bacteria and might, in perspective, pro-vide a more effective clinical alternative to the techniques of bacterial typing of the subgingival plaque

Young people, young adults and Binge Drinking

Introduction and Purpose The consumption of alcohol among young people and young adults has undergone, in recent decades, a sharp upsurge with the increasingly frequent intake of large quantities of alcohol. The aim of our study was to investigate socio-demographic, economic and behavioural factors that have a major impact on the voluntary alcohol habit in young people. Methods The survey was conducted via administration of an anonymous questionnaire based on “the WHO Alcohol Use Disorders Identification Test”, disseminated on-line , to young people and young adults (aged 18 to 35) Results We examined a sample of 365 subjects. Consumption of wine and beer were predominant  followed by super-alcohol mixes. We found correlations between alcohol use and the following variables: marital status (p<0.001), parental education(mother p<0.05; father p<0.001), income level(p<0.05), physical activity (p<0.05) and voluptuous habits(smoke and coffee: p<0.001.The  5% of men and 1% of women had car accidents during the previous year due to alcohol use and 15% said they did not remember what happened in an alcoholic evening once or twice a month. Conclusions The phenomenon of alcohol consummation is deeply ingrained in our reality, with dangerous episodes of binge drinking in young adults with a higher prevalence  in the female sex

Hepatitis E virus (HEV) in sewage from treatment plants of Messina University Hospital and of Messina city council

Samples of sewage from treatment plants at the ?G. Martino? University Hospital of Messina (AOU) and that of Messina City Council were analysed to detect the hepatits E virus. Samples were taken on sewage entering and exiting the treat- ment plants on a monthly basis over a one-year period from both the hospital plant (24 samples) and the municipal plant (22 samples). All sewage samples were pretreated by ultrafiltraton and concen- tration and finally processed by the PCR method to amplify gene material. A total of three samples tested positive: two (8.33%) entering the AOU treatment plant and one (4.5%) entering the municipal plant while no cases of HEV were detected in samples of treated sewage. These findings confirm the presence of the virus in the city of Messina and showed that the two treatment plants to be working efficiently when tested

Comparison between a conventional subunit vaccine and the MF59-adjuvanted subunit influenza vaccine in the elderly: an evaluation of the safety, tolerability and immunogenicity

The objective of this study was to evaluate and compare the safety, tolerability and immunogenicity for two seasonal influ- enza subunit vaccines, one with MF59 adjuvant (Fluad?) and one without an adjuvant (Agrippal?). A total of 195 subjects aged ? 65 years were enrolled to receive one dose of vaccine intramuscularly, 96 were vaccinated with Fluad?, 99 received Agrippal?. Blood samples were taken from all subjects in order to assess their antibody titre by the haemagglutination inhibition assay (HI), before (Time 0) and after (Time 1: 28 ± 7 days) vaccination, against the A/H3N2 (A/Moscow/10/99), A/H1N1 (A/New Caledonia/20/99) and B/Shandong/7/97 antigens contained in the influenza vaccine in the 2002/2003 influenza season for the northern emisphere. A good humoral antibody response was detected for both vac- cines, meeting all the criteria of EMEA. The number of subjects in whom a ? 4-fold increase in antibody titre was recorded, in comparison with the pre-vaccination value, proved to be lower in the group vaccinated with Agrippal? than in those vaccinated with the adjuvated preparation. Fluad? exhibited better immu- nogenicity than Agrippal?. This difference was probably linked to the potentiated immune stimulation exerted by the adjuvant molecules. These results take on a particular importance if we consider that the immune system is weaker in the elderly; the admin- istration of an adjuvated vaccine in such subjects is clearly preferable in that it provides greater and more prolonged protection. Both vaccines were generally well tolerated; no severe adverse events occurred in any of the subjects vaccinated, confirming the excellent safety profile of Fluad? and Agrippa

Trend of MDR-microorganisms isolated from the biological samples of patients with HAI and from the surfaces around that patient

Healthcare-associated infections (HAI) continue to be a major public health concern. A number of epidemiologically relevant HAI microorganisms are multidrug-resistant (MDR) germs that can spread rapidly and/or carry multiple resistance to antibiotics. They are the cause of high mortality and possible nosocomial epidemics. For this reason, we implemented microbiological surveillance acquiring samples from patients with HAI and environmental samples from the surfaces surrounding those patients. A retrospective study was carried out from January 2014 to December 2016 in two departments of the University Hospital in Messina, Italy: the Microbiology and the Hygiene Laboratories. A comparison was made between the microbiological isolates found on the patients and the microorganisms typed further to environmental sampling on the surfaces adjacent to the patient with HAI. There was a 24% match in 2014, 22% in 2015 and 20% in 2016 on total isolates. The most common isolates belonged to the Enterobacteriacae family: in particular, an ever-increasing trend has been registered for Klebsiella spp; Acinetobacter baumannii and multiresistant Pseudomonas aeruginosa have seen a growing trend for both patient and environmental samples. During the three years, the highest infection prevalence rate was found in Anaesthesia and Resuscitation, followed by Thoracic and Vascular Surgery

An observational study on the effects of early and late risk factors on the development of childhood obesity in the South of Italy

Background: Child obesity is today one of the greatest health emergencies, on such a large scale as to be considered a global epidemic by the WHO and, unfortunately, Italy holds the worst European record. Methods: We conducted an observational study to investigate the effects of early and late risk factors on the development of primary childhood obesity. We collected anthropometric parameters, information about early risk markers and late risk factors on a sample of 280 children from March 2016 to December 2017. Results: Statistically significant associations emerged between: child's BMI and education level of the mother (p<0.001) and the father (p<0.05); level of parents' education and qualitative variables (subjects’ physical activity level (p<0.05), consumption of carbonated beverages (p<0.05), fruit juices (p <0.05) and snacks (p<0.05); BMI and the presence or absence of a family history of obesity, DM and cardiovascular diseases. Significant linear correlations were found between weight classification (overweight/obese) and the consumption of carbonated drinks (p<0.05), snack consumption (p<0.05), physical activity levels (p<0.001) and duration of sleep in the afternoon (p<0.05). Conclusions: In our sample we found incorrect eating habits to be frequently linked to a low level of parental education; particularly for the mother, the main childcare provider

FIVE-YEAR MICROBIOLOGICAL MONITORING OF WARDS AND OPERATING THEATRES IN SOUTHERN ITALY

INTRODUCTION. Nosocomial infections are one of the greatest problems in public health. Several studies have highlighted the role played by the hospital environment as a possible source of transmission of nosocomial pathogens.METHODS. A five-year monitoring of bacterial contamination on healthcare workers hands, surfaces most closely in contact with inpatient wards, operating theatres and “at rest” and “in use” operating theatre air samples. For the samples, we used sterile swabs, contact slides, manual API, and automated VITEK systems for identification.RESULTS. In the five-year period, a total of 9396 samples were collected and analysed. In ward patients, 4398 samplings were carried out with 4.7%, 9.4%, 7%, 10.8% and 7.9% positive results respectively from 2010 to 2014. For hands, 648 samplings were carried out, with a positivity of 40.74%. In operating theatres, 4188 samples were taken, with a positivity of 11.9%. Regarding air in empty and full theatres, 1962 samplings were carried out with a positivity rate equal to 31.9%. The monitoring showed a low rate of contamination with a progressive decrease in the five-year period on operating theatres surfaces and hands, while there was an increase in the surgical site wards and in the air of operating rooms.CONCLUSIONS. Our investigation has revealed the presence of pathogens on the assessed surfaces and the need for environmental monitoring, which can be a valuable tool for reducing contamination