A mosaic-structured framework applied in the healthy food design: insights from integrated in silico and in vitro approaches

Agrifood system technologies have enabled the development of novel foods with enhanced nutritional composition. Herein, we provide an overview of food model/prototype development by placing natural compounds (NCs) as an inspiration source through the combination of multidisciplinary approaches and food technologies. To design healthy foods, we propose that these interlinked technologies derived from applied fields, such as nutrition, chemical synthesis, pharmacology and food technology should be combined with biological system analyses. Food molecule development is a slow process with mandatory steps based on desired bioactive properties, which is challenged by food matrix diversity and the difficulty of reconstituting its biosynthetic pathways. Overcoming these challenges should be the primary trend in food science, leading to more sustainable processes and health benefits.</p

Stability Indicating Method for a Thiazolylhydrazone Derivative with Antifungal Activity and Experimental/Theoretical Elucidation of Its Degradation Products

RN104 (2-[2-(cyclohexylmethylene)hydrazinyl]-4-phenylthiazole) is a thiazolylhydrazone derivative with promising in vitro and in vivo antifungal activity. A stability indicating highperformance liquid chromatography with diode-array detection (HPLC-DAD) method was carried out using C18 end-capped column (250 × 4.6 mm, 5 μm) and a mobile phase composed of water and acetonitrile (15:85 v/v) at a flow rate of 1.2 mL min-1, injection volume 25 μL and DAD detection at 240 nm. The method showed to be selective, linear in the range of 20 to 240 μg mL-1, precise, accurate and robust. RN104 forced degradation study under different stress conditions (acidic, alkaline and neutral hydrolysis, oxidation, photolysis and thermolysis) was performed using the validated analytical method. The results showed that RN104 underwent significant degradation when subjected to alkaline hydrolysis and oxidation by metallic ions. Quantum mechanics calculations were carried out to assist in the structural elucidation of the formed degradation products. The obtained data may be useful for the development of future formulation based on RN104.</div