Additional file 3: Figure S3. of The pesticidal Cry6Aa toxin from Bacillus thuringiensis is structurally similar to HlyE-family alpha pore-forming toxins

Modeled L259D mutant. The full-length structure (5KUC, magenta) is overlaid with the L259D model (cyan). The inset illustrates the putative transmembrane loop region with residue 259 shown in stick display. Loops are colored as above for clarity while, for other regions, the full-length structure is dark gray and the modeled mutant is light gray. (PPTX 737 kb

Additional file 4: Figure S4. of The pesticidal Cry6Aa toxin from Bacillus thuringiensis is structurally similar to HlyE-family alpha pore-forming toxins

Western blot of wild-type and L259D mutant Cry6Aa. Expression of the wild-type Cry6Aa (WT) and the L259D mutant confirmed by western blotting. (PPTX 1138 kb

Additional file 2: Figure S2. of The pesticidal Cry6Aa toxin from Bacillus thuringiensis is structurally similar to HlyE-family alpha pore-forming toxins

Ab initio models of Cry6Aa. The five models constructed with reference to database information (db) and five models constructed entirely de novo (dn) are shown. The predicted N-terminal domains are shown in light gray and marked “N,” while the C-terminal domains are shown in black and marked “C.” (PPTX 774 kb

Additional file 1: Figure S1. of The pesticidal Cry6Aa toxin from Bacillus thuringiensis is structurally similar to HlyE-family alpha pore-forming toxins

Multiple sequence alignment of Cry6Aa and related toxins. Sequences of full-length Cry6Aa and Cry6Ba were aligned using the ClustalX program along with the sequences from the crystal structures of HlyE [PDB: 4PHO], HblB [PDB: 2NRJ], and NheA [PDB: 4K1P]. Residues identical to the Cry6Aa sequence are highlighted in green; residues identical to Cry6Ba but not in Cry6Aa are highlighted in blue. Repeat regions in Cry6Aa are highlighted in yellow (repeat motif 1 WATIGAxIQ/E, motif 2 TTNMTSNQY, and motif 3 WYNNSD). The putative transmembrane region is highlighted in gray with L259 indicated by a blue asterisk. The “wing” features in the Cry6Aa sequence are indicated by magenta highlight. (DOCX 19 kb