The influence of ionizing radiation to the DNA-estrogen receptor complex

The influence of ionizing radiation on the stability of specific complex between DNA bearing estrogen response element and estrogen receptor protein. Protein irradited alone loses its ability to form the complex with nonirradiated DNA at much lower doses than these when the whole complex is destroyed. Theoretical model RADACK has been applied to calculate yields of damages within DNA and protein

Vliv ionizujícího záření na komplex DNA s receptorem estrogenů

Radiation damage of specific complex between DNA estrogen response element and protein estrogen receptor was studied. The overall stability of the complex and particular damages of both biomolecules under irradiation were followed

The effect of ionizing radiation on the complex of estrogen receptor with DNA

Estrogen receptor protein forms a specific complex with DNA bearing estrogen response element. The study of the effect of ionizing radiation on the stability of the complex is presented. Method of agarose gel electrophoresis was used to follow radiomodifying effects of estrogen, which is in conditions in vivo necessary for complex formation. Method of retardation polyacrylamide electrophoresis was used to determine overall stability of the complex as a function of absorbed dose

Vliv ligandů na stabilitu specifického komplexu tvořeného proteinem estrogenovým receptorem a DNA estrogenovým responsivním elementem po ozáření

The overall stability of the specific complex between estrogen receptor protein and DNA estrogen response element under irradiation has been followed. The effect of ligands, estrogen and or tamoxifen, has been determined