Clinical Isolates of <i>C</i>. <i>trachomatis</i>, the Doses Used to Infect Cell Cultures and Multiplicities of Infection Corresponding to the Doses and to the Isolates.

<p><i>IFU</i>, inclusion forming units; <i>MOI</i>, multiplicity of infection</p

Immunohistochemistry and direct immunofluorescent staining of hRPE and McCoy cell cultures at different time-points postinoculation with <i>Chlamydia trachomatis</i> (clinical isolate No.24032).

<p>In both types of cultures, both techniques reveal highly immunoreactive inclusions (arrowheads). Twenty-four to 72 h postinoculation, a reduction in the number of intracellular inclusions is observed due to the release of a new generation of EBs to the extracellular environment. Scale bar: 50 μm.</p

Primary Antibodies Used to Detect <i>C</i>. <i>trachomatis</i> Antigens, Signaling, Cytoskeleton and Extracellular Matrix Molecules.

<p><i>MOMP</i>, major outer membrane protein; <i>VEGF</i>, vascular endothelial growth factor; <i>TNF–α</i>, tumor necrosis factors–α; <i>α-SMA</i>, smooth muscle actin; <i>MMP–9</i>, matrix metalloproteinase 9; <i>bFGF</i>, basic fibroblast growth factor; <i>TGF–β</i>, transforming growth factor β; <i>RFU</i>, ready for use</p

Changes in the percentage of inclusion-containing cells in hRPE and McCoy cell cultures following inoculation with <i>Chlamydia trachomatis</i> (clinical isolate No.24032).

<p>Changes in the percentage of inclusion-containing cells in hRPE and McCoy cell cultures following inoculation with <i>Chlamydia trachomatis</i> (clinical isolate No.24032).</p

Changes in the expression of signaling molecules and proteins of cytoskeleton and extracellular matrix of hRPE cells grown in culture following inoculation with <i>Chlamydia trachomatis</i> (clinical isolates Nos. 24032 and 27692).

<p>Changes in the expression of signaling molecules and proteins of cytoskeleton and extracellular matrix of hRPE cells grown in culture following inoculation with <i>Chlamydia trachomatis</i> (clinical isolates Nos. 24032 and 27692).</p

Immunohistochemistry micrographs of hRPE cell culture at 72 hours postinoculation with <i>Chlamydia trachomatis</i> clinical isolate No.24032.

<p>(A) Intracellular inclusions (arrowhead) exhibiting weak immune responsiveness (due to release of EBs to extracellular environment). (B) Numerous EBs (arrowheads) in microscopy projection of cells without inclusions. Scale bar: 10 μm.</p