4 research outputs found
DescripciĂłn del perfil ontogĂ©nico de hormonas esteroides sexuales e intermediarios en testĂculos del ratĂłn de orejas negras (Peromyscus melanotis, Allen y Chapman, 1897)
Se analizó por radioinmunoanálisis (RIA) el contenido de esteroides sexuales (ES), dos hormonas (progesterona –P4-y
testosterona -T-) y tres intermediarios (pregnenolona -P5-; 17a-hidroxi-progesterona –17P4-y androstendiona –A-), en
cuatro categorĂas de edad de Peromyscus melanotis, asĂ como la histologĂa de sus testĂculos y epidĂdimos. Cuando se
compararon las concentraciones de los ES entre su correspondiente categorĂa de edad (CE) se encontraron diferencias
significativas (P < 0.0001), pero cuando la comparaciĂłn se hizo entre P5, P4, 17P4 y A en las cuatro CE no hubo diferencias
(P > 0.05). Al comparar el contenido de T entre subadultos (CE II) y adultos jĂłvenes (CE III) la diferencia fue significativa (t1,7
= 30.80, P
0.05); en cambio sĂ existieron (t1,7 = 14.05, P < 0.0001) cuando la comparaciĂłn se hizo entre adultos (CE IV) y viejos (CE V). El
ciclo ontogénico de la testosterona, asà como la espermatogénesis aumentan a partir de la pubertad, alcanzando su máximo
en la edad adulta y decrecen progresivamente con el envejecimiento
Identification of serotoninergic system components in stallion sperm
Assisted reproductive techniques have been
used on several domestic animals to preserve desirable
traits in strains of high genetic and commercial value;
however in equines its efficiency rate is relatively low.
To increase the conception ratio in stallions, some
research groups have used pharmacological treatments
which promote sperm hyperactivation in order to
increase male’s fertility rates. In this way, our previous
work suggests that serotonin (5-HT) could be a good
pharmacological candidate that facilitates conception
rate in domestic horses. 5-HT is a neurohormone
involved in several reproductive processes, i.e., it
enhances hyperactivation, motility, and promotes the
acrosome reaction in mammalian sperm, but it has not
been described in the stallion sperm yet. Therefore,
using both immunofluorescence and western blot
techniques, we searched for and found some serotonin
markers such as 5-HT, 5-HT1B, 5-HT2A, 5-HT3
receptors, both TPH1 and MAOA enzymes, and
serotonin transporter (5-HTT) in stallion sperm. In
addition, we found a non-neuroendocrine cell, V-MAT1
transporter, which has not been previously reported in
mammalian sperm. Our results suggest that
serotoninergic system is present in stallion sperm, which
could be a pharmacological target to increase the
conception rates in domestic horses