Assessing Cellular Uptake of Exogenous Coenzyme Q10 into Human Skin Cells by X-ray Fluorescence Imaging

X-ray fluorescence (XRF) imaging is a highly sensitive non-invasive imaging method for detection of small element quantities in objects, from human-sized scales down to single-cell organelles, using various X-ray beam sizes. Our aim was to investigate the cellular uptake and distribution of Q10, a highly conserved coenzyme with antioxidant and bioenergetic properties. Q10 was labeled with iodine (I2-Q10) and individual primary human skin cells were scanned with nano-focused beams. Distribution of I2-Q10 molecules taken up inside the screened individual skin cells was measured, with a clear correlation between individual Q10 uptake and cell size. Experiments revealed that labeling Q10 with iodine causes no artificial side effects as a result of the labeling procedure itself, and thus is a perfect means of investigating bioavailability and distribution of Q10 in cells. In summary, individual cellular Q10 uptake was demonstrated by XRF, opening the path towards Q10 multi-scale tracking for biodistribution studies

Assessing Cellular Uptake of Exogenous Coenzyme Q10_{10} into Human Skin Cells by X-ray Fluorescence Imaging

X-ray fluorescence (XRF) imaging is a highly sensitive non-invasive imaging method for detection of small element quantities in objects, from human-sized scales down to single-cell organelles, using various X-ray beam sizes. Our aim was to investigate the cellular uptake and distribution of Q$_{10}$, a highly conserved coenzyme with antioxidant and bioenergetic properties. Q$_{10}$ was labeled with iodine (I$_2$-Q$_{10}$) and individual primary human skin cells were scanned with nano-focused beams. Distribution of I$_2$-Q$_{10}$ molecules taken up inside the screened individual skin cells was measured, with a clear correlation between individual Q$_{10}$ uptake and cell size. Experiments revealed that labeling Q$_{10}$ with iodine causes no artificial side effects as a result of the labeling procedure itself, and thus is a perfect means of investigating bioavailability and distribution of Q$_{10}$ in cells. In summary, individual cellular Q$_{10}$ uptake was demonstrated by XRF, opening the path towards Q$_{10}$ multi-scale tracking for biodistribution studies