4 research outputs found
Skin Imprinting in Silica Plates: A Potential Diagnostic Methodology for Leprosy Using High-Resolution Mass Spectrometry
Leprosy is a chronic infectious disease
caused by Mycobacterium leprae, which
primarily infects macrophages
and Schwann cells, affecting skin and peripheral nerves. Clinically,
the most common form of identification is through the observation
of anesthetic lesions on skin; however, up to 30% of infected patients
may not present this clinical manifestation. Currently, the gold standard
diagnostic test for leprosy is based on skin lesion biopsy, which
is invasive and presents low sensibility for suspect cases. Therefore,
the development of a fast, sensible and noninvasive method that identifies
infected patients would be helpful for assertive diagnosis. The aim
of this work was to identify lipid markers in leprosy patients directly
from skin imprints, using a mass spectrometric analytical strategy.
For skin imprint samples, a 1 cm<sup>2</sup> silica plate was gently
pressed against the skin of patients or healthy volunteers. Imprinted
silica lipids were extracted and submitted to direct-infusion electrospray
ionization high-resolution mass spectrometry (ESI-HRMS). All samples
were differentiated using a lipidomics-based data workup employing
multivariate data analysis, which helped electing different lipid
markers, for example, mycobacterial mycolic acids, inflammatory and
apoptotic molecules were identified as leprosy patients’ markers.
Otherwise, phospholipids and gangliosides were pointed as healthy
volunteers’ skin lipid markers, according to normal skin composition.
Results indicate that silica plate skin imprinting associated with
ESI-HRMS is a promising fast and sensible leprosy diagnostic method.
With a prompt leprosy diagnosis, an early and effective treatment
could be feasible and thus the chain of leprosy transmission could
be abbreviated
Population birth data and pandemic readiness in Europe
To access publisher's full text version of this article, please click on the hyperlink in Additional Links field or click on the hyperlink at the top of the page marked DownloadThe SARS-CoV-2 pandemic exposed multiple shortcomings in national and international capacity to respond to an infectious disease outbreak. It is essential to learn from these deficiencies to prepare for future epidemics. One major gap is the limited availability of timely and comprehensive population-based routine data about COVID-19's impact on pregnant women and babies. As part of the Horizon 2020 PHIRI (Population Health Information Research Infrastructure) project on the use of population data for COVID-19 surveillance, the Euro-Peristat research network investigated the extent to which routine information systems could be used to assess the effects of the pandemic by constructing indicators of maternal and child health and of COVID-19 infection. The Euro-Peristat network brings together researchers and statisticians from 31 countries to monitor population indicators of perinatal health in Europe and periodically compiles data on a set of 10 core and 20 recommended indicators.Horizon 2020 Framework Programm
Epidemiological characteristics, practice of ventilation, and clinical outcome in patients at risk of acute respiratory distress syndrome in intensive care units from 16 countries (PRoVENT): an international, multicentre, prospective study
Background Scant information exists about the epidemiological characteristics and outcome of patients in the intensive care unit (ICU) at risk of acute respiratory distress syndrome (ARDS) and how ventilation is managed in these individuals. We aimed to establish the epidemiological characteristics of patients at risk of ARDS, describe ventilation management in this population, and assess outcomes compared with people at no risk of ARDS. Methods PRoVENT (PRactice of VENTilation in critically ill patients without ARDS at onset of ventilation) is an international, multicentre, prospective study undertaken at 119 ICUs in 16 countries worldwide. All patients aged 18 years or older who were receiving mechanical ventilation in participating ICUs during a 1-week period between January, 2014, and January, 2015, were enrolled into the study. The Lung Injury Prediction Score (LIPS) was used to stratify risk of ARDS, with a score of 4 or higher defining those at risk of ARDS. The primary outcome was the proportion of patients at risk of ARDS. Secondary outcomes included ventilatory management (including tidal volume [VT] expressed as mL/kg predicted bodyweight [PBW], and positive end-expiratory pressure [PEEP] expressed as cm H2O), development of pulmonary complications, and clinical outcomes. The PRoVENT study is registered at ClinicalTrials.gov, NCT01868321. The study has been completed. Findings Of 3023 patients screened for the study, 935 individuals fulfilled the inclusion criteria. Of these critically ill patients, 282 were at risk of ARDS (30%, 95% CI 27–33), representing 0·14 cases per ICU bed over a 1-week period. VT was similar for patients at risk and not at risk of ARDS (median 7·6 mL/kg PBW [IQR 6·7–9·1] vs 7·9 mL/kg PBW [6·8–9·1]; p=0·346). PEEP was higher in patients at risk of ARDS compared with those not at risk (median 6·0 cm H2O [IQR 5·0–8·0] vs 5·0 cm H2O [5·0–7·0]; p<0·0001). The prevalence of ARDS in patients at risk of ARDS was higher than in individuals not at risk of ARDS (19/260 [7%] vs 17/556 [3%]; p=0·004). Compared with individuals not at risk of ARDS, patients at risk of ARDS had higher in-hospital mortality (86/543 [16%] vs 74/232 [32%]; p<0·0001), ICU mortality (62/533 [12%] vs 66/227 [29%]; p<0·0001), and 90-day mortality (109/653 [17%] vs 88/282 [31%]; p<0·0001). VT did not differ between patients who did and did not develop ARDS (p=0·471 for those at risk of ARDS; p=0·323 for those not at risk). Interpretation Around a third of patients receiving mechanical ventilation in the ICU were at risk of ARDS. Pulmonary complications occur frequently in patients at risk of ARDS and their clinical outcome is worse compared with those not at risk of ARDS. There is potential for improvement in the management of patients without ARDS. Further refinements are needed for prediction of ARDS
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Search for supersymmetry in hadronic final states with missing transverse energy using the variables αT and b-quark multiplicity in pp collisions at √s = 8 TeV
Submitted by Vitor Silverio Rodrigues ([email protected]) on 2014-05-27T11:30:34Z
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Previous issue date: 2013-09-01
An inclusive search for supersymmetric processes that produce final states with jets and missing transverse energy is performed in pp collisions at a centre-of-mass energy of 8 TeV. The data sample corresponds to an integrated luminosity of 11.7 fb-1 collected by the CMS experiment at the LHC. In this search, a dimensionless kinematic variable, αT, is used to discriminate between events with genuine and misreconstructed missing transverse energy. The search is based on an examination of the number of reconstructed jets per event, the scalar sum of transverse energies of these jets, and the number of these jets identified as originating from bottom quarks. No significant excess of events over the standard model expectation is found. Exclusion limits are set in the parameter space of simplified models, with a special emphasis on both compressed-spectrum scenarios and direct or gluino-induced production of third-generation squarks. For the case of gluino-mediated squark production, gluino masses up to 950-1125 GeV are excluded depending on the assumed model. For the direct pair-production of squarks, masses up to 450 GeV are excluded for a single light first- or second-generation squark, increasing to 600 GeV for bottom squarks. © 2013 CERN for the benefit of the CMS collaboration.
CERN, Geneva
Yerevan Physics Institute, Yerevan
Institut für Hochenergiephysik der OeAW, Wien
National Centre for Particle and High Energy Physics, Minsk
Universiteit Antwerpen, Antwerpen
Vrije Universiteit Brussel, Brussel
Université Libre de Bruxelles, Bruxelles
Ghent University, Ghent
Université Catholique de Louvain, Louvain-la-Neuve
Université de Mons, Mons
Centro Brasileiro de Pesquisas Fisicas, Rio de Janeiro
Universidade do Estado do Rio de Janeiro, Rio de Janeiro
Universidade Estadual Paulista, São Paulo
Universidade Federal do ABC, São Paulo
Institute for Nuclear Research and Nuclear Energy, Sofia
University of Sofia, Sofia
Institute of High Energy Physics, Beijing
State Key Laboratory of Nuclear Physics and Technology Peking University, Beijing
Universidad de Los Andes, Bogota
Technical University of Split, Split
University of Split, Split
Institute Rudjer Boskovic, Zagreb
University of Cyprus, Nicosia
Charles University, Prague
Academy of Scientific Research and Technology of the Arab Republic of Egypt Egyptian Network of High Energy Physics, Cairo
National Institute of Chemical Physics and Biophysics, Tallinn
Department of Physics University of Helsinki, Helsinki
Helsinki Institute of Physics, Helsinki
Lappeenranta University of Technology, Lappeenranta
DSM/IRFU CEA/Saclay, Gif-sur-Yvette
Laboratoire Leprince-Ringuet, Ecole Polytechnique IN2P3-CNRS, Palaiseau
Institut Pluridisciplinaire Hubert Curien, Universite de Strasbourg, Universite de Haute Alsace Mulh CNRS/IN2P3, Strasbourg
CNRS-IN2P3, Institut de Physique Nucléaire de Lyon Université de Lyon, Université Claude Bernard Lyon 1, Villeurbanne
Institute of High Energy Physics and Informatization Tbilisi State University, Tbilisi
I. Physikalisches Institut RWTH Aachen University, Aachen
III. Physikalisches Institut A RWTH Aachen University, Aachen
III. Physikalisches Institut B RWTH Aachen University, Aachen
Deutsches Elektronen-Synchrotron, Hamburg
University of Hamburg, Hamburg
Institut für Experimentelle Kernphysik, Karlsruhe
Institute of Nuclear and Particle Physics (INPP) NCSR Demokritos, Aghia Paraskevi
University of Athens, Athens
University of Ioánnina, Ioánnina
KFKI Research Institute for Particle and Nuclear Physics, Budapest
Institute of Nuclear Research ATOMKI, Debrecen
University of Debrecen, Debrecen
Panjab University, Chandigarh
University of Delhi, Delhi
Saha Institute of Nuclear Physics, Kolkata
Bhabha Atomic Research Centre, Mumbai
Tata Institute of Fundamental Research - EHEP, Mumbai
Tata Institute of Fundamental Research - HECR, Mumbai
Institute for Research in Fundamental Sciences (IPM), Tehran
INFN Sezione di Bari, Bari
Università di Bari, Bari
Politecnico di Bari, Bari
INFN Sezione di Bologna, Bologna
Università di Bologna, Bologna
INFN Sezione di Catania, Catania
Università di Catania, Catania
INFN Sezione di Firenze, Firenze
Università di Firenze, Firenze
INFN Laboratori Nazionali di Frascati, Frascati
INFN Sezione di Genova, Genova
Università di Genova, Genova
INFN Sezione di Milano-Bicocca, Milano
Università di Milano-Bicocca, Milano
INFN Sezione di Napoli, Napoli
Università di Napoli 'Federico II', Napoli
Università della Basilicata (Potenza), Napoli
Università G. Marconi (Roma), Napoli
INFN Sezione di Padova, Padova
Università di Padova, Padova
Università di Trento (Trento), Padova
INFN Sezione di Pavia, Pavia
Università di Pavia, Pavia
INFN Sezione di Perugia, Perugia
Università di Perugia, Perugia
INFN Sezione di Pisa, Pisa
Università di Pisa, Pisa
Scuola Normale Superiore di Pisa, Pisa
INFN Sezione di Roma, Roma
Università di Roma, Roma
INFN Sezione di Torino, Torino
Università di Torino, Torino
Università del Piemonte Orientale (Novara), Torino
INFN Sezione di Trieste, Trieste
Università di Trieste, Trieste
Kangwon National University, Chunchon
Kyungpook National University, Daegu
Institute for Universe and Elementary Particles Chonnam National University, Kwangju
Korea University, Seoul
University of Seoul, Seoul
Sungkyunkwan University, Suwon
Vilnius University, Vilnius
Centro de Investigacion y de Estudios Avanzados del IPN, Mexico City
Universidad Iberoamericana, Mexico City
Benemerita Universidad Autonoma de Puebla, Puebla
Universidad Autónoma de San Luis Potosí, San Luis Potosí
University of Auckland, Auckland
University of Canterbury, Christchurch
National Centre for Physics Quaid-I-Azam University, Islamabad
National Centre for Nuclear Research, Swierk
Institute of Experimental Physics, Faculty of Physics University of Warsaw, Warsaw
Laboratório de Instrumentação e Física Experimental de Partículas, Lisboa
Joint Institute for Nuclear Research, Dubna
Petersburg Nuclear Physics Institute, Gatchina (St. Petersburg)
Institute for Nuclear Research, Moscow
Institute for Theoretical and Experimental Physics, Moscow
P.N. Lebedev Physical Institute, Moscow
Skobeltsyn Institute of Nuclear Physics Lomonosov Moscow State University, Moscow
State Research Center of Russian Federation Institute for High Energy Physics, Protvino
Faculty of Physics and Vinca Institute of Nuclear Sciences University of Belgrade, Belgrade
Centro de Investigaciones Energéticas Medioambientales y Tecnológicas (CIEMAT), Madrid
Universidad Autónoma de Madrid, Madrid
Universidad de Oviedo, Oviedo
Instituto de Física de Cantabria (IFCA) CSIC-Universidad de Cantabria, Santander
European Organization for Nuclear Research CERN, Geneva
Paul Scherrer Institut, Villigen
Institute for Particle Physics ETH Zurich, Zurich
Universität Zürich, Zurich
National Central University, Chung-Li
National Taiwan University (NTU), Taipei
Chulalongkorn University, Bangkok
Cukurova University, Adana
Physics Department Middle East Technical University, Ankara
Bogazici University, Istanbul
Istanbul Technical University, Istanbul
National Scientific Center Kharkov Institute of Physics and Technology, Kharkov
University of Bristol, Bristol
Rutherford Appleton Laboratory, Didcot
Imperial College, London
Brunel University, Uxbridge
Baylor University, Waco
The University of Alabama, Tuscaloosa
Boston University, Boston
Brown University, Providence
University of California, Davis, Davis
University of California, Los Angeles
University of California, Riverside, Riverside
University of California, San Diego, La Jolla
University of California, Santa Barbara, Santa Barbara
California Institute of Technology, Pasadena
Carnegie Mellon University, Pittsburgh
University of Colorado at Boulder, Boulder
Cornell University, Ithaca
Fairfield University, Fairfield
Fermi National Accelerator Laboratory, Batavia
University of Florida, Gainesville
Florida International University, Miami
Florida State University, Tallahassee
Florida Institute of Technology, Melbourne
University of Illinois at Chicago (UIC), Chicago
The University of Iowa, Iowa City
Johns Hopkins University, Baltimore
The University of Kansas, Lawrence
Kansas State University, Manhattan
Lawrence Livermore National Laboratory, Livermore
University of Maryland, College Park
Massachusetts Institute of Technology, Cambridge
University of Minnesota, Minneapolis
University of Mississippi, Oxford
University of Nebraska-Lincoln, Lincoln
State University of New York at Buffalo, Buffalo
Northeastern University, Boston
Northwestern University, Evanston
University of Notre Dame, Notre Dame
The Ohio State University, Columbus
Princeton University, Princeton
University of Puerto Rico, Mayaguez
Purdue University, West Lafayette
Purdue University Calumet, Hammond
Rice University, Houston
University of Rochester, Rochester
The Rockefeller University, New York
Rutgers The State University of New Jersey, Piscataway
University of Tennessee, Knoxville
Texas A and M University, College Station
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Vanderbilt University, Nashville
University of Virginia, Charlottesville
Wayne State University, Detroit
University of Wisconsin, Madison
Universidade Estadual Paulista, São Paul