1 research outputs found
Oxygen Nanobubbles-Embedded Hydrogel as Wound Dressing to Accelerate Healing
Herein, we propose an oxygen nanobubbles-embedded hydrogel
(ONB-G)
with carbopol for oxygenation of wounds to accelerate the wound healing
process. We integrate carbopol, hydrogel, and dextran-based oxygen
nanobubbles (ONBs) to prepare ONB-G where ONBs can hold and release
oxygen to accelerate wound healing. Oxygen release tests showed that
the proposed ONB-G could encapsulate oxygen in the hydrogels for up
to 34 days; meanwhile, fluorescence studies indicated that the ONB-G
could maintain high oxygen levels for up to 4 weeks. The effect of
carbopol concentration on the oxygen release capacity and rheological
features of the ONB-G were also investigated along with the sterility
of ONB-G. HDFa cell-based studies were first conducted to evaluate
the viability, proliferation, and revival of cells in hypoxia. Scratch
assay and mRNA expression studies indicated the potential benefit
for wound closure. Histological evaluation of tissues with a pig model
with incision and punch wounds showed that treatment with ONB-G exhibited
improved healing compared with hydrogel without ONBs or treated without
the gel. Our studies show that dextran-shell ONBs embedded in a gel
(ONB-G) have the potential to accelerate wound healing, given its
oxygen-holding capacity and release properties