Restructuring of Pancreatic Islets and Insulin Secretion in a Postnatal Critical Window

Function and structure of adult pancreatic islets are determined by early postnatal development, which in rats corresponds to the first month of life. We analyzed changes in blood glucose and hormones during this stage and their association with morphological and functional changes of alpha and beta cell populations during this period. At day 20 (d20), insulin and glucose plasma levels were two- and six-fold higher, respectively, as compared to d6. Interestingly, this period is characterized by physiological hyperglycemia and hyperinsulinemia, where peripheral insulin resistance and a high plasmatic concentration of glucagon are also observed. These functional changes were paralleled by reorganization of islet structure, cell mass and aggregate size of alpha and beta cells. Cultured beta cells from d20 secreted the same amount of insulin in 15.6 mM than in 5.6 mM glucose (basal conditions), and were characterized by a high basal insulin secretion. However, beta cells from d28 were already glucose sensitive. Understanding and establishing morphophysiological relationships in the developing endocrine pancreas may explain how events in early life are important in determining adult islet physiology and metabolism

Islet composition changes in pancreatic head and tail at d6, 20 and adults.

<div><p>Photomicrographs illustrate the overall distribution of alpha (red) and beta (green) cells in representative islets of the head (A–C) and body/tail (D–F) at d6 (A and D), d20 (B and E) and adults (C and F).</p> <p>Scale bar  =  50 µm.</p></div

Insulin secretion at d20 and d28 explored by RHPA.

<div><p>(A) Bar graphs represent insulin secretion index at d20 and d28, in response to different glucose concentrations, mean ± SEM, n  =  4 experiments by duplicate, (*) p<0.05 with respect to 15.6 mM.</p> <p> <b>(B)</b> Percentage of insulin secreting beta-cells from the total population as identified by insulin immunocytochemistry after the RHPA; mean ± SEM, n  =  4 experiments by duplicate, **p<0.0001 with respect to d20.</p> <p> <b>(C)</b> Pancreatic beta cell subpopulations at d20 and d28 in response to basal, 5.6 mM glucose.</p> <p>Data pooled from 3 experiments by duplicate.</p></div

Structural changes of pancreatic beta and alpha cells during the first month of life.

<div><p> <b>(A)</b> The mass of beta cells in the head and tail of the gland.</p> <p> <b>(B)</b> Frequency distribution of beta cell aggregates at d6 and d90.</p> <p> <b>(C)</b> Comparison between d20 and d90.</p> <p>Data were pooled from 8 rats, n  =  9 216 beta cell aggregates measured.</p> <p> <b>(D)</b> Mass of alpha cells in the head and tail during the first month of life.</p> <p> <b>(E, F)</b> Frequency distribution of the size of alpha cell aggregates at d6, d20 and d90.</p> <p> <b>(E)</b> Comparisons between d6 and d90. <b>(F)</b> Comparisons between d20 and d90.</p> <p>Data were pooled from 4 rats, n  =  1 739 alpha cell aggregates measured, are expressed as mean ± SEM.</p> <p>Symbols denote statistically significant differences: (*) p < 0.05 with respect to previous age.</p></div

Additional file 1: of Development of a core outcome set for clinical trials in squamous cell carcinoma: study protocol for a systematic review of the literature and identification of a core outcome set using a Delphi survey

SPIRIT checklist. Completed checklist of the study protocol for the development of a core outcome set. (DOCX 48 kb